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Sandesh earned his Ph.D. in Biotechnology in 2020, from the Institute of Chemical Technology, Mumbai. He is currently a Postdoctoral Researcher at the University of Tennessee Health Science Center (UTHSC), Memphis, US. His research interests include immunology, pharmacognosy, food bioscience, and functional foods and he is passionate about scientific writing.
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You had been happily measuring your DNA samples with your NanoDrop for a while, until… you noticed that your results are not reproducible *gulp*.
Read on – here are 10 points that will help you troubleshoot the issue.
For identifying positive clones from a plasmid cloning procedure, the routine of performing a mini-prep and then checking the putative clones by restriction digestion is most commonly used. Of course, if you need to screen a large number of clones, another option is a colony PCR to identify positives, followed by restriction digests to confirm….
The whole TLC technique sounds easy to do, but it can be difficult and tricky during interpretation or give unexpected results, especially when working with biomolecules. For this reason, it is important to be familiar with troubleshooting thin layer chromatography. Some of the common problems faced during TLC and their solutions are listed below: Solvent…
It’s often said that great leaders have a knack for bringing out the best in those that follow. In turn, followers enjoy the work they do and will take the initiative to soar far above and beyond what is asked of them. A less effective scientific leader may unknowingly squander potential that might have flourished…
Pulling an over-nighter is a common theme for many of my college friends. I, however, like my sleep—particularly between midnight and 7 am. Therefore, I tend not to stay up late to study and avoided jobs that required me to work a night shift. Then, I went to grad school and had to put in…
You’ve tried all the usual stuff, and checked the primer sequences twice, but still can’t get that PCR fragment amplified. It’s time to enter the strange world of PCR additives. Over the years a variety of additives have been shown to enhance PCR reactions in certain situations. Here is a summary of some of the…
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