Join Us
Sign up for our feature-packed newsletter today to ensure you get the latest expert help and advice to level up your lab work.
Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
…in BaseSpace at some point. The real benefit of the MiSeq QC is that it gives a real sequence based quality check of your whole experimental process before you ask…
…biophysical techniques. Surface Plasmon Resonance (SPR) tracks binding kinetics in real time. Isothermal Titration Calorimetry (ITC) measures the heat released during binding. Radioligand binding assays (RBA) use displacement of labeled…
…a consequence, PCR primers incorporating LNAs can enhance PCR amplification sensitivity under a wide range of conditions—LNAs have been shown to enhance real-time PCR and DNA sequencing. [4] 2. Expanding the Genetic Alphabet Perhaps the most straightforward…
…delay time data for proton NMR spectra. Seven spectra were collected at ~1-millisecond intervals. (Image credit: Jennifer Cable.) Note: These are not real data. No real data ever look this…
…information could be gleaned from sequencing, even if it was being performed for another purpose. Real life example Because the information associated with the ACMG variant list was actionable, and…
…the well-established, romantic image of the dedicated scientist who loves his/her work above anything else. So what is the real story? Do scientists really like, or even love, their jobs…
…can be challenging, but it’s an emerging field of functional genetics and genomics—with real prospects to identify new disease-associated pathways and therapeutic targets. To date, there have been tens of thousands…
…what they are asking. Anticipating these possibilities will help you prepare beforehand. That way you can diagnose the question in real time so you can better address what the questioner…
…LgBiT delivery enables intracellular expression of LgBiT, which supports live-cell assays that capture real-time protein degradation kinetics. NanoBRET measures protein interactions rather than abundance. This assay detects bioluminescence resonance energy…
…eliminates the use of stationary PCR machines for amplification. Imagine a pocket-sized nano sequencer that you can bring to any places. This scenario would not be part of the scientific…
…law. This, however, is the ideal situation – the best case scenario. In real life, resolution must be defined in terms of contrast, and there are additional parameters that have…
…successful, you should observe every aspect of the fish. Although it might be true that for real love physical appearance does not matter, body shape is very informative when it…
…a very real effect. Clean, sharp gels and high signal-to-noise ratios in antibody-dependent techniques inspire confidence in the reviewers, which influences where the paper will be published. So-called ‘pretty’ images…
…went back to talk to the careers service people. They gave me mock interviews for jobs prior to a real interview and this really helped. Because it wasn’t real, it…
Most PhD students will agree that the process of doing your PhD is challenging, but this overlooks a very real struggle that starts before you even step foot into the…
…are many reasons. But the real enemy here is ignorance. And the real problem? The sheer volume of information we each store in our DNA: Humans have 46 chromosomes, and…
…assay Positive 4 1 Negative 1 4 \(Sensitivity = \frac{\mathrm{TP} }{\mathrm{TP+FN}} = \frac{\mathrm{4} }{\mathrm{4+1} } = 4/5 = 80\%\) Not too bad, right? Shall we look at a real-world example?…
…for another go at it and the X-ray beam is narrow enough. It’s common to see the diffraction pattern deteriorate enough in real-time during the diffraction experiment because of free…
…gained a valuable piece of information about what life might be like working for them. The only real rule here is don’t start the conversation by asking them if they…
…that is available to anyone with a gmail account. It provides standard editing functionality and really excels in real-time collaborative authoring. Here are our favorite GoogleDocs features, which were all…
…nucleic acid-based tests rely on real-time polymerase chain reaction (RT-PCR) to amplify reverse-transcribed viral RNA. The resulting PCR amplicons are then detected using standard RT-PCR detection methods. These tests are…
…real. Therefore you need to ensure you have a suitable sample size. Paired or unpaired data One factor that will be important in determining which type of test to use…
…(2009). The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 55(4):611-22. doi: 10.1373/clinchem.2008.112797. Originally published on January 20, 2009. Revised and updated in April 2026….
…consensus among studies was also partly the reason why the Cochrane reviews were initiated. What can you do to ensure tight error bars, reproducibility, and measurements that reflect “real” (or…
…real-time RT-PCR. Nature Protocols, 1 (3): 1559- 1582 M. Wacker & M. Godard (2005) Analysis of One-Step and Two-Step Real-Time RT-PCR Using SuperScript III. J Biomol Tech, 16 (3): 266–271…
…be subtracted from both your bands of interest and the bands you are normalizing to. Perfection here is challenging; just do your best and let statistics tell you the real…
…Bustin S. A. et al. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments Clinical Chemistry (2009), 81(55), 611– 622 Church G and Gilbert W. Genomic sequencing….
…Are “Real” Controls First, let’s all get on the same page. Flow cytometry is dependent on the detection of fluorophores conjugated to antibodies directed against a specific antigen. Antibodies (or…
…planned. This is a good time to get to know people or discover the town. Discussion Panels and Discussion Leaders: Allows students/post-docs/young investigators to ask real honest questions without judgment…
…In a cryo-EM experiment, electrons are scattered when they hit the sample. Electrons are negatively charged. This charge can be exploited to focus the scattered electrons electromagnetically in real-time to…
…addition, all that personal attention and investment in your success can add up to a lot of pressure, real or only perceived. Suddenly a grant deadline looms, and that assay…
…inclusion levels can be calculated as the percentage of the isoform with the alternative exon divided by the total abundance of the two isoforms. Real-time qPCR Alternatively, real-time qPCR can…
Hydrolysis probes, commonly also referred to as TaqmanTM, are a very popular chemistry for real-time quantitative PCR (RT-qPCR). In this article, I will compare hydrolysis probes with PlexorTM. But first,…
Want to measure how much mRNA you have in a particular sample? Easy! Make some cDNA, add some fluorescent DNA-intercalating dye, pop it into a real-time quantitative PCR (RT-qPCR) machine,…
…extra steps, like end-repair, A-tailing and adaptor ligation steps. After synthesis of the second strand, cDNAs are selected by size and amplified by PCR and the resulting library is ready…
The eBook with top tips from our Researcher community.
