Techniques / Cells and Model Organisms

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Article

Six Steps to Successful Mouse Genotyping

An essential step in mouse breeding is genotyping them to determine the genotype of every mouse in the litter. It is also useful to differentiate between various groups of experimental mice if any confusion...
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Webinar

Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability

In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components. We will cover: How quality custom reagents in small...
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Article

Sonication – 7 Tips for Mastering the art

Sonication is mostly used during preparation of protein extracts to help break apart the cell. Although most lysis buffers have buckets of detergent that lyse cell membranes, sonication just gives an extra hand...
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Article

How to Extract and Amplify Microalgal Genomic DNA

There is a growing trend in using microalgae as the expression system for heterologous proteins. However, I find most protocols dealing with microalgae available online are not that great or informative! So I...
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Article

How to Disinfect Plant Explants for Tissue Culture

Prior to using plant explants, the surface of the explant needs to be disinfected to prevent introduction of unwanted microbes into your culture. Usually, you use chemicals (called sterilant liquids) at...
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Article

Murine Matchmaking: Starting a Breeding Colony of Mice

Mice are an extremely powerful research tool, but they do take extra time and effort. While you can purchase mice from vendors like Jackson Labs or outsource your genetic engineering to a number of companies,...
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Article

For the Love of Mouse! How to Start Working with Mice

Researchers have always been in search of model organisms that can be used to study and explore biological phenomena to make discoveries that can be extrapolated to more complex higher organisms like humans. Of...
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Article

The Messy World of Human Tissue Research

In neuroscience and other biomedical research fields, animal models allow us to answer critical but otherwise impossible questions. Despite the value of these models, however, sometimes nothing can replace the...
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Article

Open and Closed: Two Ways to Grow Your Own Algae

In my last article, I talked about the basic protocols and experiments conducted in the process of converting algae into biofuel. Our ability to culture algae has efficiently improved over the years. Continuous...
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Article

World of Microbes Part 3: Antibody Production with Microbes

When you think of microbes what comes to mind? Moldy bread, Penicillin and antibiotics? Vaccines? Fermented food, like yogurt and kombucha? And the latest Probiotics health craze? How about immune therapy?...
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