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Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
…Generation of double-stranded cDNA Library amplification Library quantification Sequencing. You can measure differential gene expression by comparing read count data from different experimental groups. For example, a drug-treated sample compared…
…Library of Medicine. Finding and Using Health Statistics. National Library of Medicine (US). Available from: https://www.nlm.nih.gov/oet/ed/stats/index.html Rosner, B. 2011. Fundamentals of biostatistics (7th ed.). Brooks/Cole, Cengage Learning. References Chesher D….
…running, they move to “Testing:” Measure expression from the promoter library in E. coli Compare the expression across plasmid A and B Optimize induction conditions This approach gives you and your…
…researchers to screen massive libraries of peptides, sometimes with as much as 1011 total unique peptides! You can then use this library for such things as identifying novel binding partners…
…the Illumina method is the amplification of template molecules on a solid surface. The DNA sample is prepared into a “sequencing library” by the fragmentation into pieces each around 200…
…to reveal the transcripts you care about? cDNA synthesis – What priming method will you use to make cDNA from your RNA? Library prep – Will you use stranded or…
…were sequenced. It took around 50 hours to complete the sample and library preparation, sequencing and analysis. These samples were sequenced on a rapid track Illumina Hiseq 2500, 2×100 read length…
You know those ridiculously priced and throw-away DNA mini, midi and maxi-prep columns? Well the good news is that you can actually re-use them if you are reasonably careful at…
…URL: www.covaris.com/ffpe-extraction. (Accessed April 4, 2025) McCarthy P, Beker L, Vasantgadkar S, Pundt E, Daviso E, Thomann U, Bhattacharyya D. (2024 June 2-6). Comprehensive Sample Prep Workflow for Deparaffinization, Extraction,…
…your own RNA included in the prep.) Rather than being helpful, the glycogen content in tissue samples can interfere with silica column based purification. Basically, in a tissue preparation, you…
…preparation. Check out the Bitesize guide to sample prep for SEM here and read about fixing samples for TEM here. What about the differences between SEM and TEM? I’m guessing…
…of the process. The insert prep methods were: 1. T4 DNA polymerase treatment. Just like the vector, the insert could be subjected to T4 DNA polymerase treatment (without dNTPs) to…
…a good idea to use midi or maxi-prep, to produce enough for upcoming experiments. Are Your HEK293 Cells Happy? It is essential to maintain cells in good condition. Cells should…
For identifying positive clones from a plasmid cloning procedure, the routine of performing a mini-prep and then checking the putative clones by restriction digestion is most commonly used. Of course,…
…to be shuffled, you can do a quick plasmid prep if the gene is cloned in to a plasmid. Then, you can use restriction enzymes to cut out the sequence…
…run. Heat can be a double-edged sword for PAGE. Some additional heat can help denature proteins that haven’t been fully denatured during sample prep. Still, too much heat can make…
…making nuclease treatment more affordable and practical for both small labs and large-scale operations. Learn More From clogged filters in a protein prep to regulatory barriers in a biopharma and…
…provide Mass Spec newbies with an excellent introduction to mass spectrometry. We’ve also covered how to select the best matrix and prep your sample, for MALDI (Matrix-Assisted Laser Desorption/Ionization) experiments,…
…(Figure 2). Although cryofixation is fast (milliseconds) and provides the best preservation of your sample, cryo-EM sample prep and imaging are challenging and specialist equipment is required to prepare, store,…
…mice give lower yields and more spontaneous maturity. Mice that are injured, unhealthy or immune-compromised show effects on all immune cells, including DC numbers and differentiation. The prep – Bone…
…Cells can be very sticky, and it doesn’t take much to block the instrument—especially if you have poor sample prep. Also, people forget the importance of cleaning the cytometer after…
…drift (temperature, CO₂, humidity), a medium prep or storage error, an osmolality or pH shift, a degraded supplement, a difference in coating preparation, or population drift and senescence. In conclusion……
Isn’t it a pain to digest, purify, and dephosphorylate your cloning vector prep to prevent high background in your ligation/transformation? A new generation of positive selection cloning vectors promises to…
…technique to use more conventional dyes such as AlexaFluor488 and 647, which makes experiment design and sample prep easier. How does this help you? The advantage of STORM over PALM…
…concentration of impurities in your prep may be too high. Try 5-10 parallel reactions with concentrations from 10 to 200 ng in a 50 microlitre reaction. Check the cycler –…
…The workflow includes DNA size-selection via the BluePippin instrument as a key part of the sample prep process for the 10x Genomics Chromium platform used for library sequencing. This process…
…use a reliable kit designed specifically for the preparation of cDNA libraries for strand-specific RNA sequencing. Enter the InvitrogenTM CollibriTM Stranded RNA Library Prep Kit for IlluminaTM with CollibriTM Human/Mouse/Rat…
…cells must be carefully optimized to prevent their loss during sample prep. Since in many cases the read-out in this assay is a smear, an antibody-based method may produce artifacts…
…what you want to do next with your cells, may not be possible. 6. Prep your cells correctly Make sure there are no clumps in your cell suspension as this…
…the end use of your sample. Are you going to do PCR? Avoid using heparin for blood collection. Are you using your samples for RNA prep? Immediately add trizol or…
…the CyTOF include: Slower acquisition compared to traditional flow cytometry – maximum of about 1,000 events per second Sample prep must be cleaner than traditional flow cytometry – all biological…
…it Repeat pipettors, multi-channel pipettors, vacuum assemblies even automated DNA prep machines are all great for handling lots of samples, but take the time to learn how to use them….
…a liquid. You need to prep the device by passing the same buffer as required for downstream applications through the machine to ensure equilibration and absence of blockages. Viscous cell…
…also a real concern about sequencing adapter-dimers, a common byproduct of the sample prep process, because they can load preferentially and waste sequencing capacity on useless DNA. Most NGS platforms…
…be aware of: Destructive chemical processing: Sample prep involves fixation, polymerization, and digestion, making it incompatible with live-cell imaging. This means you get a snapshot of a fixed state, not…
…at all). Prep a Few Steps Beforehand This might not always be feasible, but if you have the option, do some preparatory steps before even reaching the collaborating lab, which…
…maximum advantage of your prep and minimize waste, aliquoting requires knowing the concentration of your sample and how much you are likely to need in future experiments, so that the…
…careful library preparation (including DNA size selection) to obtain. Then again, you are not going to let that stop you from getting your hands on the high-quality data sets, are…
…a library or doing a particularly tricky ligation, because efficiency could be somewhat reduced. For example, individual cells that were close to dividing at the time of transformation might not…
…PET to image in whole organisms. Pretty cool. Competitive ABPP as Alternative to Click Chemistry ABPP Consider this scenario: You screened a compound library and found a molecule that potently…
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