Techniques / Protein Expression & Analysis

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Ten Ways to Give Your Surface Plasmon Resonance Experiments a Hand

Surface Plasmon Resonance (SPR) is the gold standard for measuring biomolecular binding without the need for labeling (i.e., label free detection of kinetics). SPR is especially valuable because it doesn’t...
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Article

Have a BLAST With Your Protein Sequences

When I was being trained in microbiology as an undergrad, one of the first skills I acquired was the ability to quickly compare and visualize amino acid sequences using BLAST and ClustalW. 15 years later, those...
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Article

Stripping Blots – It’s All Fun and Games Until Someone Loses Their Protein

Like all technical fields, molecular biology contains a very robust “theoretical” realm and an equally robust “practical” realm. Unfortunately, these two existences don’t seem to overlap as often as...
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Article

SDS-PAGE Tips and Tricks to Save You Time

Today, SDS-PAGE is one of the basic methods in biolabs. As a beginner in my lab, I had little help, so my first SDS-PAGE gel took two whole days! Since then, I’ve learned a lot about how to make the process...
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Article

How to Cherry Pick Your Primary Antibody

How do you pick which antibody you should use in your assay? If you’re starting a new assay and need an antibody for the job, then selecting a new antibody from the plethora available could be high up on your to-do list.
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Webinar

Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability

In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components. We will cover: How quality custom reagents in small...
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Article

A Beginner’s Guide to Tag-Removing Proteases

Every biochemist is familiar with proteases. More often than not, proteases cause a lot of anxiety. To this end, a lot of research has been done in developing techniques to prevent the activity of proteases....
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Article

Common Mass Spectrometry Contaminants: I Messed It Up So You Don’t Have To!

Through many trials, and lots of error, I learned that there are many considerations for mass spectrometry that might not be obvious to you as a molecular biologist. Common contaminants, even in small quantities, can mask important peaks in your mass spec data and have a huge impact on the final results.
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Article

How to Use Proteases to Purposefully Digest Proteins

In this article I will not talk about ‘wild’ proteases, which destroy cellular proteins in your lysates like wolves destroy sheep. Instead, I’ll be talking about the shepherd dog proteases—purified,...
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Article

Soluble Sample Success: How to Optimize Protein Solubility

Put in the time now to optimize protein solubility so you don’t have to suffer later! With this in mind, here are some considerations and tips for selecting the optimal conditions for recombinant protein expression and purification.
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