Techniques / Protein Expression & Analysis

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Article

How to Cherry Pick Your Primary Antibody

How do you pick which antibody you should use in your assay? If you’re starting a new assay and need an antibody for the job, then selecting a new antibody from the plethora available could be high up on your to-do list.
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Webinar

Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability

In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components. We will cover: How quality custom reagents in small...
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Article

A Beginner’s Guide to Tag-Removing Proteases

Every biochemist is familiar with proteases. More often than not, proteases cause a lot of anxiety. To this end, a lot of research has been done in developing techniques to prevent the activity of proteases....
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Article

Common Mass Spectrometry Contaminants: I Messed It Up So You Don’t Have To!

Through many trials, and lots of error, I learned that there are many considerations for mass spectrometry that might not be obvious to you as a molecular biologist. Common contaminants, even in small quantities, can mask important peaks in your mass spec data and have a huge impact on the final results.
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Article

How to Use Proteases to Purposefully Digest Proteins

In this article I will not talk about ‘wild’ proteases, which destroy cellular proteins in your lysates like wolves destroy sheep. Instead, I’ll be talking about the shepherd dog proteases—purified,...
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Article

Soluble Sample Success: How to Optimize Protein Solubility

Put in the time now to optimize protein solubility so you don’t have to suffer later! With this in mind, here are some considerations and tips for selecting the optimal conditions for recombinant protein expression and purification.
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Article

Bis-Tris Gels: Sharpen Up Your Protein Bands

A sprinkling of Bis-tris is all it takes improve your protein gels considerably. If you can afford it!
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Sponsored Education

How SDS-PAGE works

What does a stacking gel do? This and other questions about SDS-PAGE answered!
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Article

Isolating Proteins? Use Histidine and Transition to Metals

To isolate your protein, you are going to need to tag it with something that will allow you to fish it out of the bacterial protein soup. This is where transition metals can help you out!
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Article

How to Make a Custom Affinity Medium for Protein Purification

Is your goal to purify a substantial amount of a specific protein? Do you have a quantity of a molecule that binds your protein of interest? If so, generating a custom affinity matrix may be just the trick you...
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