Techniques / Flow Cytometry

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Article

A Biologist’s Guide to Choosing Your Fluorophore Palette

You may notice that nature is full of vibrant, even fluorescent, colors. The human eye detects wavelengths ranging from 390-700 nm and our perception of colors is actually a narrow part of the light spectrum....
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Article

How to Destroy your Flow Cytometry Data in 3 Easy Steps: Snap, Crackle, and Pop

While many scientists are methodical and precise, some of us like to live on the edge. Read a protocol all the way through? No thanks, I’ll take my chances and guess what concentration of HCl I should use....
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Article

Flow Cytometric Apoptosis Assays for Cell Death

Apoptosis, often called programmed cell death, is a carefully regulated process that is part of normal development and homeostasis. Apoptosis is morphologically and biochemically distinct from necrosis, which...
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Article

Top 5 Tricks for Using FlowJo

Are you planning to do cellular immunology research?  Then chances are you will be introduced to the flow cytometer –  “a modern immunologist’s best friend.” This modern magic box is a highly...
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Sponsored Education

Ensure Reproducibility: Control for Lot-to-Lot Variation of Antibodies

When starting a long-term experiment, you need to take a lot of things into consideration (availability of cells, reagents, planning time points), but do you ever think about your antibodies? If you buy an...
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Product

BD OptiBuild™ Custom Reagents are Unlocking Science with New Antibody and Dye Combinations

The need for multicolor flow cytometry is increasing all the time. We want to look at more markers, in smaller samples, perform quicker acquisition, have simpler post-acquisition analysis and, oh wait, can we...
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In Flow Cytometry
Article

Introducing CyTOF: Cytometry of the Masses

Flow cytometry remains unparalleled as a single-cell analysis technology.  The ability to analyze 14 or more fluorescent parameters on a million cells or more allows for detailed understanding of complex...
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Article

Mass(ively powerful) Cytometry

Mass Cytometry is a relatively new technology which has recently featured in many high-impact journals. You may have read about instruments including the CyTOF, CyTOF2, and more recently, the Helios. With...
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Article

Cell Cycle Analysis by Flow Cytometry: Flowing your Way through Life’s Cycle

Over the past few decades the mammalian cell cycle has been well documented. Although there are lots of checkpoints as cells move through the cycle, we can very simply divide the cell cycle into three stages...
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Article

Gateway to the Cellular Kingdom: Cell Fixation and Permeabilization Techniques

One of the much sought after question asked by many researchers worldwide is – “What is the gene expression profile of a single cell within a heterogenous pool of cells?” While mass cytometry is the...
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