Chasing the Pot of Gold at the End of the Rainbow: Choosing the Right Fluorochromes for Your Flow Cytometry

Chasing the Pot of Gold at the End of the Rainbow: Choosing the Right Fluorochromes for Your Flow Cytometry

With the current proliferation of new dyes and instruments that can detect many colors simultaneously, it seems like an entire rainbow is at your disposal for your flow cytometry experiments. And we know that when designing a polychromatic flow cytometry panel, more is often better – right?  The more antigens you can detect, the more…

Cell Proliferation Round 1: Using Thymidine Analogs With Flow Cytometry

Cell Proliferation Round 1: Using Thymidine Analogs With Flow Cytometry

Around and around the cell cycle goes, where it stops, nobody knows. Unless you have the right tools to analyze DNA content, that is. The DNA markers propidium iodide, Hoechst and DAPI are commonly used in flow cytometry to analyse a cell’s DNA content.  Although they are simple to use, they do have disadvantages. Figure…