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…obtained, it is a good idea to use midi or maxi-prep, to produce enough for upcoming experiments. Are Your HEK293 Cells Happy? It is essential to maintain cells in good…
For identifying positive clones from a plasmid cloning procedure, the routine of performing a mini-prep and then checking the putative clones by restriction digestion is most commonly used. Of course,…
…quick plasmid prep if the gene is cloned in to a plasmid. Then, you can use restriction enzymes to cut out the sequence you want to shuffle. You will avoid…
…run. Heat can be a double-edged sword for PAGE. Some additional heat can help denature proteins that haven’t been fully denatured during sample prep. Still, too much heat can make…
…(Figure 2). Although cryofixation is fast (milliseconds) and provides the best preservation of your sample, cryo-EM sample prep and imaging are challenging and specialist equipment is required to prepare, store,…
…mice give lower yields and more spontaneous maturity. Mice that are injured, unhealthy or immune-compromised show effects on all immune cells, including DC numbers and differentiation. The prep – Bone…
…Cells can be very sticky, and it doesn’t take much to block the instrument—especially if you have poor sample prep. Also, people forget the importance of cleaning the cytometer after…
Isn’t it a pain digesting, purifying and dephosphorylating your cloning vector prep to eliminate prevent high background in your ligation/transformation? A new generation of positive selection cloning vectors promises to…
…makes experiment design and sample prep easier. How does this help you? The advantage of STORM over PALM is that you have control over antibody concentrations, and may be able…
…template concentration may be too low or the concentration of impurities in your prep may be too high. Try 5-10 parallel reactions with concentrations from 10 to 200 ng in…
…The workflow includes DNA size-selection via the BluePippin instrument as a key part of the sample prep process for the 10x Genomics Chromium platform used for library sequencing. This process…
…a reliable kit designed specifically for the preparation of cDNA libraries for strand-specific RNA sequencing. Enter the InvitrogenTM CollibriTM Stranded RNA Library Prep Kit for IlluminaTM with CollibriTM Human/Mouse/Rat rRNA…
…cells must be carefully optimized to prevent their loss during sample prep. Since in many cases the read-out in this assay is a smear, an antibody-based method may produce artifacts…
…what you want to do next with your cells, may not be possible. 6. Prep your cells correctly Make sure there are no clumps in your cell suspension as this…
…prep? Immediately add trizol or RNAlater. In some cases one works better than the other, so test it out with your samples first. Are you working with tissue samples that…
…CyTOF include: Slower acquisition compared to traditional flow cytometry – maximum of about 1,000 events per second Sample prep must be cleaner than traditional flow cytometry – all biological material…
…it Repeat pipettors, multi-channel pipettors, vacuum assemblies even automated DNA prep machines are all great for handling lots of samples, but take the time to learn how to use them….
…a liquid. You need to prep the device by passing the same buffer as required for downstream applications through the machine to ensure equilibration and absence of blockages. Viscous cell…
…also a real concern about sequencing adapter-dimers, a common byproduct of the sample prep process, because they can load preferentially and waste sequencing capacity on useless DNA. Most NGS platforms…
…First, you need to prep your cDNA and purify your cDNA: Step 1: Isolate your mRNA and perform reverse transcription using reverse transcriptase and biotinylated primers to generate the corresponding…
…at all). Prep a Few Steps Beforehand This might not always be feasible, but if you have the option, do some preparatory steps before even reaching the collaborating lab, which…
…do this for unstable buffers. To take maximum advantage of your prep and minimize waste, aliquoting requires knowing the concentration of your sample and how much you are likely to…
…with sample prep for mass spectrometry (MS) and can be difficult to stain for analysis. This means IEF must almost always be done first so that SDS-PAGE can make the…
gif by Kronin Okay not talking about that kind of matrix. But the kind of matrix that is crucial for MALDI (Matrix-Assisted Laser Desorption Ionization). I talked briefly about MALDI…
…Hub for tips and tricks for all your histology experiments. You might also be interested in the later steps of sample prep for histology, which are covered in our eBook…
Generating a DNA vector library of single and/or compound mutants for a target protein can be a daunting task. If you’re lucky and work in a well-funded lab, you might…
…many different commercial and non-commercial transfection reagents available. Bear in mind that if you need to prep lentivirus regularly, commercial transfection kits can be an expensive option. Cheap chemical reagents…
…careful library preparation (including DNA size selection) to obtain. Then again, you are not going to let that stop you from getting your hands on the high-quality data sets, are…
…a library or doing a particularly tricky ligation, because efficiency could be somewhat reduced. For example, individual cells that were close to dividing at the time of transformation might not…
…PET to image in whole organisms. Pretty cool. Competitive ABPP as Alternative to Click Chemistry ABPP Consider this scenario: You screened a compound library and found a molecule that potently…
…bias with this method, but because the size-range is narrower than nebulization or sonication (and there is no sample loss with Covaris shearing), much more DNA is available for library…
…be done using a candidate protein approach to test a few interacting pairs, or in a high-throughput format by using a screening library to identify novel binding partners. Interactions between…
…XXXIV – Of a Flea. An illustration of a flea’ from Robert Hooke’s ‘Micrographia’ (1665). Image source- National Library of Wales, Wikimedia Commons. (Public domain) The Father of Microbiology…
…product is a combinatorial library composed of various combinations of mutated plasmids. Very straightforward and convenient! Have you tried TPCR yet? Sources: Erijman A, Dantes A, Bernheim R, Shifman JM,…
…got together one Saturday, booked a room in the library, and decided to see what happened if we worked as a group. By far this was the best decision of…
…to be successful in the lab. It used to be (long before my time) that you had to stroll down to the library once a week to browse through the…
…therefore, consists of a pre-designed library of synthetic nucleic acid probes that are immobilized and spatially arrayed on a solid matrix. The Evolution of DNA Microarrays Microarrays evolved from a…
…NGS library quantification, increased sensitivity for detecting target in limited clinical samples and improved multiplexing capability. Here’s how dPCR works The initial dPCR reaction is assembled using the same reaction…
…techniques described here, RNA measurements obtained by the bioanalyzer are relatively uninfluenced by contamination. Techniques such as northern blotting, cDNA library construction and cDNA microarray analysis require long RNA fragments…
…10.1021/acssynbio.5b00296 Thermo Fisher Scientific. Overview of Protein Expression. Protein Biology Resource Library. Shimizu Y, Kanamori T, Ueda T. Protein synthesis by pure translation systems. Methods. (2005) 36(3):299-304. DOI: 10.1016/j.ymeth.2005.04.006 Shoba….
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