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Step-by-Step Planning Before You Write
…plan before we write: to write clearly and coherently. Without planning your scientific paper or grant, then your text won’t follow a logical progression to your conclusion – and you’ll…
Read More How to Write a Killer Research Interest Statement
…or around 400 words. Pay attention: do not rewrite your CV – clearly define your research interests. Highlight your scientific skills, your passion, and your ideas! How should you structure…
Read More Three-Dimensional Scanning Electron Microscopy for Biology
…have been produced on an ultramicrotome. Serial block face SEM captures images of the sample between removing slices using a diamond knife. Focused ion beam SEM removes slices using an…
Read More How to Choose the Correct Reverse Transcription Method
…oligo-dTs may not give the same result as priming using random hexamer primers. Therefore, you should use the same conditions for each RT reaction. In addition when using SYBR chemistry…
Read MoreAn Easy Way to Start Using R in Your Research: Making Pretty Plots With ggplot
…scatter plot would use identical code and I figured it was easier to keep using the data we had been using. Of course, all these plots can be ‘prettified’ just…
Read More Level Up Your Drug Screening With CRISPR
…(Cas9) is a well-established system that manipulates individual genes in a targeted manner using guide RNAs (gRNAs). Scaling CRISPR methods through the creation of gRNA libraries enables large genome-wide screens…
Read More How RNA-FISH Can Complement qPCR, and Vice Versa
…DNA (cDNA) using a reverse transcriptase. This is followed by qPCR using fluorescent probes to measure and quantify the amplification of the target sequence. The Benefits of Using RNA-FISH and…
Read More Spinning Around: Tips and Tricks for Using Centrifugal Filters
…more area for non-specific binding. Reusing Used Centrifugal Filters Because of the cost, it is compelling to use MWCO filters mulitple times. If you wish to do so, after each…
Read More Quantifying & Assessing RNA: TE or not TE?
…Tris-EDTA (TE) buffer to dissolve (or elute, if you are using column purification) RNA. When I was trained in assessing RNA using UV spectrophotometry, graduate students just shrugged at me…
Read More Top Tips for Reducing PhD Nightmares
…really don’t want to be in the position of having to retype your whole PhD thesis word-by-word ….again! 6. Brighten up your workspace Your workspace will be a huge a…
Read More How To Write an Awesome Abstract
…limit: Make sure that you check the journal’s guidelines for the word limit of the abstract before you get too carried away. I usually write up a draft including everything…
Read More How to Write a Press Release to Impress
…is actually part of their job to help you get your research out, and they have invaluable insight into how to write a press release, who to send it to,…
Read More Trapping Objects With Light: From Star Wars to Neurons
…manipulation of single cells using infrared laser beams. Nature 1987, 330, 769–771. Ashkin, A.; Dziedzic, J.M. Internal cell manipulation using infrared laser traps. Proc. Natl. Acad. Sci. USA 1989, 86,…
Read More How to Perform DNA Extraction from Dried Blood Spots Using Chelex Resin
…isolating DNA from such samples using BT Chelex 100 Resin3 is described below. Step One: Erythrocyte Lysis Cut out a dry blood spot and place it into a microcentrifuge tube…
Read MoreUsing Flow Cytometry for Fluorescence Resonance Energy Transfer
A marriage of sorts Fluorescence resonance energy transfer, or FRET, is often done using a microscope, which means it can be difficult to analyze large numbers of cells in one…
Read More HPTLC: Basics and Instrumentation
…on the plate using a Derivatizer. Finally, the compounds are quantified by scanning the plate using a scanner. Let’s delve into each of these instruments in detail. HPTLC Instrument Parts:…
Read More Cell Proliferation Round 1: Using Thymidine Analogs With Flow Cytometry
…There are standard ways of doing this using either strong acid or alkali, heat or enzymatic treatment. What can BrdU do for you? Using BrdU, you can more easily identify…
Read More Respect the Ultra
…without causing a noticeable effect on the tube. If you are using thin-walled sealed tubes, the tube must be completely full or it will collapse. Only open-top tubes in swing-bucket…
Read More Explained: Sensor Chips for Surface Plasmon Resonance and Other Applications
…two most widely used methods — covalent coupling- and affinity-based capture. Covalently Coupled Biosensor Chips Covalent immobilization of the ligand or target is carried out using the amine, thiol, aldehyde,…
Read More Five Simple Tips to Break Your Dissertation up into Manageable Parts
…your dissertation. Starts With a Bang Blog. University of Leicester Student Learning Development. Writing a dissertation. Wolfe, Joe. How to write a PhD thesis. Grammar girl’s quick and dirty tips….
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