A Simple SDS-PAGE Gel Recipe and 10-Step Casting Protocol for Perfect Gels
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Read MoreHow Filtration Works: A Short Guide for Biologists
If you’ve worked in a lab, odds are you’ve had an encounter with filtration of one sort or another. Do you understand exactly how filters work, though? Or have you wondered why certain filters are used for certain lab applications?
Read MoreHow To Get Organized With Reference Managers for Science—ReadCube Papers
Discover more about how ReadCube Papers can help you keep on top of your references.
Read MoreA Protein Renaturation Toolkit: 21 Tips and Tricks for Refolding Proteins
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
Read MoreProtein Staining Methods: An Overview of 3 of the Best
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Read MoreHow to Get Organized With Reference Managers for Science—EndNote
Learn more about the key features of EndNote to work out if it’s the right reference manager for you.
Read MoreFlow Cytometry Gone Viral: Introducing Flow Virometry
Do you want to know about a cool way to detect and tell the difference between virus particles? Then read on to discover flow virometry!
Read MoreHow To Get Organized With Reference Managers for Science—Mendeley
Learn how Mendeley allows you to organize a library containing your favorite articles and generate citations and bibliographies while writing papers.
Read MoreTop 10 Best Science Podcasts For Researchers
Facing hours of cell culture, or some other repetitive and mundane lab task? Why not cheer things up with a podcast or two! Discover our top 10 favorite science podcasts for researchers, from Sawbones to The Life Scientific.
Read MoreA Beginner’s Guide to Biocuration—and How to Become a Biocurator
Discover more about biocuration, why it’s so important, and what it takes to become a biocurator. It turns out that biocuration as a career choice is easier than you might think!
Read MoreEthidium Bromide: Understanding the Dangers
This article reassesses the dangers of using ethidium bromide and compares some supposedly safer alternatives
Read MoreScanning Electron Microscopy: 6 SEM Sample Preparation Pointers for Successful Imaging
Discover 6 critical scanning electron microscopy sample preparation points you need to know to get the best out of your SEM.
Read MoreMeltdown: A Hot Introduction to the Thermal Shift Assay
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Read MoreProtease Inhibitors 101: How They Work and How to Use Them
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Read MoreHomology Modeling in the World of Proteins
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
Read MoreLiquid Nitrogen Safety In The Lab: 10 Tips To Avoid LN2 Dangers
Liquid nitrogen isn’t just cool, it’s potentially deadly. We share our 10 tips for staying safe while working with liquid nitrogen.
Read MoreUsing Gage R&R Studies to Measure Precision in the Lab
Don’t just take in on faith that your measurements are accurate. Discover how to verify your measurements using gage R&R studies.
Read MoreHow to Make Your Own Chemically Competent Cells
We’ll show you how to make a DIY stock of chemically competent E. coli, the workhorse in the molecular biology laboratory.
Read MoreHow to Write a Thoughtful Discussion for Your Scientific Paper
Having trouble writing the discussion section for your scientific paper? Help is at hand. Read our do’s and don’ts to help you have your paper polished up in no time.
Read MoreUsing PubCrawler: How to Speed Up Your Literature Searches by Crawling
PubCrawler automatically searches PubMed and GenBank for queries that you specify, and emails you the results, helping you stay on top of your literature searches.
Read MoreLaemmli Buffer: What Is It for Anyway?
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
Read MoreIsoelectric Focusing: A Simple Way to Enhance Your Protein Separation
Isoelectric focusing is a way of separating proteins and peptides based on their isoelectric point. Read all about this method and its applications right here.
Read More5 Easy Ways to Tell Salt and Protein Crystals Apart
How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.
Read MoreMore Bad Laboratory Chemicals, and What They Do to You
It’s all too easy to forget how many common lab chemicals are dangerous. Read on, as we bring you even more dangerous lab chemicals that you should not be messing with.
Read MoreUseful Tips to Keep on Top of New Literature
Keeping track of new literature can be a time-consuming process. Here are some helpful hints and tips to help you keep up to date.
Read MoreXeno Nucleic Acids: Essential Tools in Synthetic Biology and Your Research
We’re all familiar with DNA and RNA. But have you heard of xeno nucleic acids (XNAs)? Read on to find out how they can be applied in biological research, and how you can start using them in your experiments.
Read MoreHow DNA Extraction Kits Work in the Lab
Understanding how DNA extraction kits work is the key to troubleshooting your extraction issues.
Read MoreLight-up RNA Aptamers: Illuminating the World of RNA
Discover how you can visualize that notoriously difficult molecule, RNA using light-up RNA aptamers (LURAs).
Read MoreTen Bad Chemicals in the Lab and What They do to You!
With hazards all around, it’s easy to forget how deadly even common chemicals in the lab are. But don’t worry—we’ve compiled a list of common, dangerous chemicals to help you out.
Read MoreIntroduction to DNA Microarrays
This is the first installment in the DNA microarray series where I will introduce the technology and explain the basics.
Read MoreChoosing the Right Bacterial Strains for Your Lab
Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.
Read MorePractical Application of Phenol-Chloroform Extraction
Are you struggling with your phenol-chloroform extraction or just looking to maximize the nucleic acid you retrieve? Then read our expert advice to improve your technique.
Read MoreThe Basics: How Phenol Extraction of DNA Works
Phenol extraction is a common method for removing proteins from nucleic acids. Discover how phenol extraction of DNA works.
Read MoreAll Charged Up: The Basics of Ion-Exchange Chromatography
Ion-exchange chromatography is used to separate and purify proteins based on net charge at a particular pH. Here are the basics about this technique.
Read MoreTissue Processing For Histology: What Exactly Happens?
Tissue processing for histology is a key step between fixation and embedding. We take you through the steps of tissue processing in this simple guide.
Read MoreHow to Measure and Improve Lab Accuracy and Precision
Accuracy and precision are critical for achieving reliable and reproducible results. Read on to discover what these terms mean and how to improve your accuracy and precision.
Read MoreMeasuring Protein Concentration More Accurately
Confused about measuring protein concentration? We walk you through three main methods and how to choose between them.
Read MorePerforming Pipette Calibration Yourself
It is necessary to check pipette calibration every few months to ensure accuracy by dispensing the right volumes. Learn how to do-it-yourself!
Read MoreUsing a Gel Filtration Chromatogram to Estimate Molecular Weight
Do you need a way to estimate the molecular weight of a sample or protein? We walk you through one simple solution using a gel filtration chromatogram.
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