Search results for: aseptic technique
Red light/Green Light In Aseptic Technique: When Is The Flame OK?
…are indeed! Good aseptic technique is often accompanied by some healthy paranoia (DON’T TOUCH MY BUGS WITH YOUR BUGGY HANDS!) but there are times when you need to quench your…
Read More Lifecycle of a Scientific Technique
…discover that the postdoc positions you are interested in list the technique as optional in the job ad. You finally get the boss’s permission to use the technique. The job…
Read More Studying the Epigenome by Next Generation Sequencing
…regarding methylation in a short time. We are going to discuss some of the techniques that use the NGS platform to study methylation (Table 1)! Table 1 – Different techniques…
Read More Multiplex Ligation-dependent Probe Amplification (MLPA)
Multiplex ligation-dependent probe amplification (MLPA) is a molecular technique developed by MRC-Holland back in 2002. In a nutshell, MLPA is a sensitive technique that allows quantification of nucleic acid sequences,…
Read More Tips for Successful Bone Marrow Isolation
…a difficult technique. Flash forward to a few weeks later and I was confidently undertaking bone marrow isolation. Learning a new technique is always daunting, but with practice and a…
Read More Southern (blot) exposure remains a useful technique
…(and even blotting). But sometimes an old technique can still uncover some new genetic tricks! REFERENCES Brown, T.A. (2001). Southern blotting and related DNA detection techniques. Encyclopedia of Life Sciences…
Read More Detecting Post-Translational Modifications
…partially transferred to a suitable membrane. PTMs are then immunodetected using fluorescence tagging. Other staining techniques include silver or fluorescent protein. One drawback to western blotting is that the technique…
Read More The Next Big Thing: Alternative Polyadenylation
…identify polyadenylation sites precisely. 1 However, there are some great NGS-based techniques developed specially for polyadenylation site identification, using diverse approaches. The goal of these techniques is to enrich the…
Read More Color Transmission Electron Microscopy
…imaging technique that produces a 2D image using electrons instead of photons. TEM works by shooting electrons through the sample, and its resolution is so fine it can image individual…
Read More What is Sterile? Find Your Way around a Sterile Tissue Culture Hood
…being free from biological contaminants. Aseptic technique will ensure that only the things you want to grow in your culture will. If you are mindful of sources of contamination and…
Read MoreThe Sandwich Technique: How to Dish Out Critique
…gently correcting your mistakes. There’s a “sandwich technique” used in education; a protocol of sorts for providing constructive feedback. It recommends starting any assessment with praise, having critical remarks in…
Read More Getting Started with Raman Spectroscopy: What You Need to Know
Are you an assiduous biologist who prefers label-free imaging methods for biological samples analysis? Raman spectroscopy offers you a wonderland of imaging technique with unlimited benefits. To start with, Raman…
Read More iPOND, Part 1: Fishing for Proteins with DNA as Bait
…proteins are present at DNA during or after replication, iPOND is an elegant technique to help you find out. In this article, I will explain how iPOND works and how…
Read More Basic Bacterial Culturing Practices
…combat this problem, sterility and aseptic technique are some of your most critical allies. Sterilize Your Bacterial Culture Tools Before Use! You can easily sterilize utensils, liquids and agar media…
Read More Trapping Objects With Light: From Star Wars to Neurons
…important in the field of biology, with a new generation of techniques collectively spawned and derived from the continuous improvement of Askhin’s single-beam optical traps, also known as Optical Tweezers….
Read More Western Blot, ELISA, SPR, Biosensor Assay or PCR: Which Technique Should I Use?
…a very attractive technique for a number of reasons. In contrast to Co-Ip, SPR does not require you to label your protein of interest. This allows you to bypass the…
Read More 17 Ways to Stop Pipetting Errors From Ruining Your Experiments
…they may be, but the accuracy of your micropipettes depends on you. You need to maintain your pipettes, practice good technique, and have an understanding of how they work. Only…
Read More A Beginner’s Guide to Exosome Isolation
…new therapeutics. If this article intrigued you, and you want to study these phenomenal tiny vesicles, remember that each isolation technique has its advantages and disadvantages. Each technique exploits one…
Read More The A-Z of PCR Variants
The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar…
Read More Oligo Purification Methods: How, Why and for What?
…your goal is, and how robust is your technique. If you are still not sure which one to choose, seek help from scientific papers that have used the technique you…
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