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How to Measure FRET

…called donor dequenching, this technique measures increased donor emission when the acceptor is photobleached. Fluorescence Lifetime Imaging Microscopy FRET (FLIM FRET) – This technique detects fluorescence lifetime changes of donor….

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SAGE Part 2: LongSAGE, RL-SAGE and SuperSAGE

SAGE, or serial analysis of gene expression, is a technique that enables you to digitally analyze the entire gene expression profile of a cell(s). Before this technique, scientists were limited…

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Color Transmission Electron Microscopy

…San Diego, has recently developed an exciting new technique, whereby TEM samples themselves can express color. The importance of this new technique is that it is the sample itself speaking….

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The Sandwich Technique: How to Dish Out Critique

…gently correcting your mistakes. There’s a “sandwich technique” used in education; a protocol of sorts for providing constructive feedback. It recommends starting any assessment with praise, having critical remarks in…

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A Short History of Cryo-Electron Microscopy

…image the objects listed above amongst many others. The same reasoning applies to the atomic world. For over a century, X-ray crystallography has been the dominant technique used to capture…

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Basic Bacterial Culturing Practices

…To combat this problem, sterility and aseptic technique are some of your most critical allies. Sterilize Your Bacterial Culture Tools Before Use! You can easily sterilize utensils, liquids and agar…

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A Beginner’s Guide to Exosome Isolation

…new therapeutics. If this article intrigued you, and you want to study these phenomenal tiny vesicles, remember that each isolation technique has its advantages and disadvantages. Each technique exploits one…

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The A-Z of PCR Variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar…

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The History of PCR

…a kitchen table, the ingredients of PCR were waiting for someone to scream out “CAKE!” and open up the scientific community to a technique with a myriad of applications. The…

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Six Steps to Successful Mouse Genotyping

…of mice. Even though mouse genotyping is a simple enough technique, once standardized, here are a few specific tips to a hassle free genotyping experience: 1. Know What you are…

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