Search results for: FACS
Herzenberg and the Invention of the FACS Machine
…talk about fluorsescent flow cytometry and scientists almost always refer to flow cytometers as “FACS machines”, the acronym “FACS” is trade-marked and owned by Becton-Dickinson. (1)https://en.wikipedia.org/wiki/Flow_cytometry (2)https://www.millipore.com/publications.nsf/a73664f9f981af8c852569b9005b4eee/f2854208fb10a9e7852575e700577a9c/$FILE/PB3167EN00.pdf (3)Herzenberg, L….
Read MoreHerzenberg and the Invention of the FACS Machine
…talk about fluorsescent flow cytometry and scientists almost always refer to flow cytometers as “FACS machines”, the acronym “FACS” is trade-marked and owned by Becton-Dickinson. (1)https://en.wikipedia.org/wiki/Flow_cytometry (2)Guava ViaCount Reagent…
Read MoreAre Quantum Dots Any Good for Flow Cytometry?
…the lab you may find them in photovoltaics or electronic displays, in the lab they can be used for fluorescence-activated cell sorting (FACS) or fluorescence microscopy. Quantum Dots in FACS…
Read MoreFive Things That Irritate Flow Cytometrists
…years ago. Unfortunatley the information is often overlooked and inaccurately described. Cells Were Examined on a FACS Despite very many explanations, this one still persists. FACS is an acronym; it…
Read More3 Ways to Use Flow Cytometry for Your Activation Experiment
…L.L. Founding father of FACS: Professor Leonard A. Herzenberg. PNAS. 2013; 110(52): 20848-20849. doi: 1073/pnas.1321731111. Lanier, L.L. Just the FACS. J Immunol. 2014; 193(5): 2043-2044. doi: 4049/jimmunol.1401725 Pearce, E.L. and…
Read MoreEvolution Flow: The Historical Background of Flow Cytometry
…Becton Dickinson (BD) and the FACS-1 was launched. BD in fact still owns the trade name FACS. The FACS-1 could measure forward scatter and fluorescence above 530nm. The machine also…
Read MoreUsing Flow Cytometry for Fluorescence Resonance Energy Transfer
…sitting. One way to overcome this, is by combining FRET with fluorescent-activated cell sorting (FACS), giving you a high-throughput method to screen for protein interactions in a vast array of…
Read MoreSorting Large Cells and Materials by Flow Cytometry
…pressure is adjustable from 5-75 p.s.i. FACS Influx: 70, 86, 100, and 140 µm tips. Optional: 200 µm tip. Sheath pressure is adjustable from 1-90 p.s.i. FACS Jazz: 100 µm…
Read MoreHow to Validate a CRISPR Experiment
…both cases, delivery is confirmed by the presence of the fluorophore in your cells using either fluorescence-activated cell sorting (FACS) or microscopy. Fluorophore expression also allows you to calculate the…
Read MoreTips for Successful Bone Marrow Isolation
…here and here. Density gradient centrifugation is also an option for isolating specific cell types (i.e. neutrophils, mononuclear cells). Fluorescence-activated cell sorting (FACs) can be used to isolate specific cell…
Read MoreGateway to the Cellular Kingdom: Cell Fixation and Permeabilization Techniques
…Triton-X or methanol to attain accessibility through the nuclear membrane. Tips & Tricks If you use FACS tubes to perform staining, the quantity of the buffers needed is large. One…
Read More7 Top Tips to Make the Most of Your Flow Cytometry Training
…cells in. This is usually PBS or FACS buffer (PBS plus FBS and EDTA). What tubes to bring your cells in (e.g., ‘FACS’ tubes: 5ml round bottom tubes, Eppendorfs, etc.)….
Read MoreLight-up RNA Aptamers: Illuminating the World of RNA
…Unfortunately, it’s highly unstable at 37? and requires a tRNA scaffold to function in vivo. [1,7] Combining SELEX with FACS using a derivative of Spinach, Spinach2, as the selection starting…
Read MoreHow Good Is Your Best Friend? A.K.A. Have You Validated Your Antibody?
You use your antibody frequently, maybe even every day. You rely on it for western blotting, immunohistochemistry, FACS, ELISA, and immunoprecipitation. You’d be lost without it. But how well do…
Read MoreTop Five Methods for Primary Antibody Labeling
In any application that uses antibodies for signal detection (e.g., Western blotting, ELISA, immunohistochemistry, or FACS), there are two approaches to antibody labeling: direct and indirect labeling. Standard Western blotting…
Read More6 Common Lab Uses of Detergents
…you need to stain those inconvenient intracellular markers for FACS, detergents are there for you. But don’t reach for harsh detergents like SDS! A mild membrane solubilizer like Tween 20…
Read MoreBuying a Secondary Antibody: Why all the Choices?
…are conjugated to fluorescent dyes and are used in FACS experiments, in immunofluorescent detection of proteins in cells or tissues and more recently, in Western blot analysis using CCD detection….
Read MoreA Simple Way to Measure T cell Killing Activity In Vivo
…“high” verses CFSE “low” target cells are analyzed by flow cytometry. Figure 1 illustrates what a typical FACs analysis plot would look like. Step 7. Calculating the percentage of specific…
Read MoreThe CS&T Report: Its Troubles, and How to Fix Them
…BD Biosciences BDTM CS&T beads user guide, 04/2015 BD Cytometer Setup and Tracking Application guide for BD FACS Digital Flow Cytometer, June 2007 Cossarizza A et al. (2019) Guidelines for…
Read MorePost-sorting Checks and Measures
…culture till they are in suitable numbers and try to enrich for the target population with a magnetic sorter. Then, continue with FACS to get better purity and/or yield. Getting…
Read MoreSorting Single Cells – What Do You Need to Consider?
…18:446-454. Rinke C, Lee J, Nath N, Goudeau D, Thompson B, Poulton N, et al. (2014). Obtaining genomes from uncultivated environmental microorganisms using FACS-based single-cell genomics. Nat. Protoc. 9: 1038-1048….
Read MoreHow to Design a CRISPR Cas9 Experiment and Start Genome Editing
…Tracking of Indels by Decomposition (TIDE CRISPR Analysis), Next-Generation Sequencing, or Fluorescence-Activated Cell Sorting (FACS). The optimal screening method depends on the nature of the modifications you made and your…
Read MoreLevel Up Your Drug Screening With CRISPR
…can be performed by either antibiotic selection or fluorescence-activated cell sorting (FACS), depending on whether an antibiotic resistance gene or fluorophore is present in the expression vector. 3. Drug Selection…
Read MoreThe 3 Most Common Flow Cytometry Fallacies
…to capture raw flow cytometry data (i.e. FACS Diva) also calculate and make a compensation matrix without the user ever having to lift a finger. Still, many researchers feel the…
Read MoreCRISPR Gene Editing: Considerations and Getting Started
…transduced cells via fluorescence-activated cell sorting (FACS). Antibiotic resistance genes that enable the selection of the transduced cells using an appropriate antibiotic. Selection markers are not limited to vector-mediated CRISPR…
Read MoreHow To Fix Isolated And In Situ Primary Cells
…kind of primary cells you are after, you may then need to do some sort of sorting by size or by FACS before proceeding. 6) Fix isolated primary cells to…
Read MoreHow to Best Improve Your Lentivirus Titer
…is placed downstream of an IRES sequence. In this situation, you may have plenty of virus transduction but if the fluorescence signal is weak, you risk underestimating virus titer. FACS…
Read MoreLocating Your Cellular Apoptosis Squad: Annexin V Staining Assays
…stage of cell death. Figure 1. Dot plot diagram from FACs showing progression of cell death. Q1. Double negative (Annexin V and 7-AAD negative) healthy cells. Q2. Annexin V positive,…
Read MoreSmall Particles (Things) Matter!- Introducing Nanoparticle PCR
…Nanoparticle-Assisted Polymerase Chain Reaction Technology. In Bio-Nanotechnology, edited by Debasis Bagchi CNS,IChE CN, nashi Bagchi FACN, Hiroyoshi Moriyama FACN, and Fereidoon Shahidi FRSC FACS, FAOCS, FCIC, FCIFST, FIAFoST, FIFT, 97–106….
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