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Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
…contamination will worsen during storage Re-prep Prominent nicked band, weak supercoiled Re-prep Re-prep Fast band below supercoiled (denatured ssDNA) Redo prep Redo prep Band in the high-molecular-weight region (possible dimer…
…prep kit for Illumina systems make it possible for library generation to keep pace with instrument speeds. The Collibri PS DNA Library Prep Kit requires only 0.4 hour hands-on time…
…and Nanodrop measurements. Nanopore library quality mostly depends on the individual researcher’s technical skills and pipetting technique. In this article, we discuss some pointers to perform a successful NGS library…
…Library Prep Kit for Illumina Systems and Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina Systems, it is possible to reduce these biases. References Macintyre G, Yistra B, and…
Making a Next Generation Sequencing (NGS) library can seem a bit daunting to the new user, as failures can be expensive. But don’t be put off, as NGS library preparation…
…approaches. © 2015 Agrotis and Ketteler [5], licensed under CC BY 4.0. MOI, multiplicity of infection; sgRNA, single guide RNA. Pooled Library Screens A pooled library can include thousands of…
…to obtaining high-quality WGS results and is available exclusively from the Invitrogen™ Collibri™ PS DNA Library Prep Kit and the Invitrogen Collibri PCR-Free PS DNA Library Prep Kit for Illumina…
…to your Mendeley library. This saves time and means that your library is effortlessly maintained and that you won’t lose that one vital paper that may hold the key to…
…the nitty-gritty of sample preparation, let’s look at the entire cryo-EM sample prep workflow (Figure 1). Figure 1. Cryo-EM sample prep workflow. (Image credit: Thomas Warwick.) Figure 1 gives you…
…Short pieces of DNA left over from the library prep process can interfere with the emulsion PCR. Small products bind to the capture bead preferentially, competing with the binding of the…
…molecule. In a semi-synthetic library, these framework regions are amplified and cloned into the phage display library from B-cells, in differing combinations to achieve different goals. In a synthetic library,…
…then randomly introduce mutations into the gene sequence to create a “library” containing thousands of versions of the gene. Each version (or “variant”) of the gene in the library would…
…plasmid preps. Prep type Plasmid DNA recovered (μg) Mini prep 5–50 Midi prep 50–200 Maxi prep 200–1000 What Causes Low Plasmid Yields? We received a question from Bitesize Bio reader…
…from your EM experiments. Advice You Can Trust To bring you the best EM sample prep advice, we interviewed EM expert Dr. Andreia Pinto who has not gone longer than…
…library of publications, collaborate easily with colleagues, and seamlessly insert citations in your chosen word processor. Maybe even more importantly, gone are the days of manually writing and formatting your…
…Identify which library holds the journal issue you need. Your institution’s library staff can usually do this search for you. Submit an interlibrary loan request through your library’s online system….
…Collibri PS DNA Library Prep Kit for Illumina systems provide visual feedback that allows real-time tracking of the successful mixing of reagents at each step in the library preparation and…
…library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome project, the MiSeq system does offer the option of…
…Managers Reference managers such as Zotero, Mendeley, JabRef, and Lean Library Workspace (formerly SciWheel) will all handle a library, generate a bibliography in a very large range of journal and…
…such as: library generation for your references; cloud syncing of your library; access across devices; and the ability to make notes, highlight text, and annotate PDFs. In addition, the sleek…
…cDNA strand, single-stranded RNAs and RNA-DNA hybrids are removed by RNAses. The second cDNA strand is then synthesized by PCR using a poly(A)-anchored primer (PAAP). 1,4 Collibri RNA Library Prep…
…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that includes…
Much more than just an archive, The Cell: An Image Library-CCDB (Cell Centered Database; ‘The Cell’) serves many additional purposes. Whilst many researchers use The Cell to organize their own…
…I will discuss five mistakes that will no doubt hype your paranoia about the possible ways to screw up your RNA prep. 1. Not keeping the working environment RNase-free The…
…distribution. Threshold varies by library–prep workflow Northern blot / cDNA library 8 ≥9 Requires long intact fragments (>1 kb); very sensitive to degradation These are practical starting points, not universal…
…with size selection for small RNAs, ligation of selected fragments to adapters, and then generation of cDNA.4 New RNA Library Preparation Method A new RNA library prep method creates cDNA—but…
…cDNA synthesis is performed. The resulting double-strand cDNA is used as the input to a standard Illumina library prep which includes end-repair, adapter ligation and PCR amplification to give you…
…concerned with reproducible library preps, gel purification after adaptor ligation is the step that requires the most attention to detail (see James Hadfield’s excellent instructions of what you need to…
…essential to ensure the integrity of the starting sample by assessing DNA quality, shearing profiles, and library size. One way to do this is to analyze HMW DNA sequencing libraries…
In Part I of AAV Production, I described how to produce crude (non-purified) AAV. In this article, I am going to tell you how to purify that crude prep. Virus…
…preps. Which is not good. Not good at all. And if the prep goes spectacularly wrong then you can end up with a prep full of the single-stranded stuff, which…
Introducing the Zymo-Seq RiboFree® Total RNA Library Kit Building up a library of RNA sequences is an excellent way to identify conserved and unique gene expression across species. However, when…
…(next generation) sequencing of cDNA 5) in silico sequence alignment1, 2. This article is about the two first stages of making an RNAseq library (steps 1- 3). Starting Material Preparation…
…prior to sample prep. Whew! That was a lot. Maybe most importantly, you should have everything set up for sample prep day. And plan your experiments carefully with your facility manager…
…to start thinking about how to perform good PALM sample prep. In this article, we will cover how to choose which fluorescent proteins to use for PALM, and then go…
…SEM sample prep points to maximize your chances of successful SEM imaging. Need to take a step back? Read our ‘Applications of Electron Microscopy‘ article to learn more about how…
…Qiagen kit at only $5.16 per Tempus Tube. But the RNA kit is $15 per prep when you buy the larger 24-prep kit. Plus, it appears a specialized piece of equipment, an…
…and I got an update from multiple users at the UK Genome Science conference. Library prep methods are pretty standard: DNA, frag, end repair, A-tail, adapter ligation. Sequencing accuracy is…
…size selection is an essential step in library preparation for consistently generating reliable results. HLA Typing Using NGS Requires DNA Size Selection for Library Preparation In the protocol released in…
…and help learn more about the expression profile of cells. Let’s take a look at the steps: First, you need to prep your cDNA and purify your cDNA: Step 1:…
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