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How to Get Started with Algae

…depending on the species. You will have to take this into consideration when starting your cultures. To optimize conditions, grow algae in conical flasks containing 100 mL of media. Media

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The Care and Keeping of Primary Murine B Cells

…all of your experiments. Make sure you make your media fresh. Media components, like glutamine, are critical for cellular growth and proliferation but can deteriorate quickly in the media. Alternatively,…

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Keeping Track of Your Literature

…tools, read our article on How to Speed Up Your Literature Searches by Crawling. Social media is also another very important tool for finding and keeping up with the latest…

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Feeder Cells – the What, Why and How

…[2] As inactivated feeder cells begin to die after 2-3 days, it is necessary to keep on replenishing the co-culture with more treated feeder cells and fresh media. Inactivating Feeder…

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How to Write a Press Release to Impress

…keep scientists from reaching out to the media and, by extension, the public. Sharing your science is beneficial to both the public and your own research. Being interviewed or writing…

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10 Easy Ways to Wreck your Autoclave

…Not using secondary containment. Is your media bottle sitting directly on the rack or the chamber floor? When bottles burst (and sometimes they do), glass shards and molten media can…

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How to Maintain Live Cells on a Microscope Stage

…also allow perfusion of different types of media. Full microscope enclosure: For dedicated live cell imaging systems, consider the full heated enclosure (if you have enough money!) that contains specimen,…

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The 10 Unspoken Rules of Working in a Lab

…mean EVER use someone else’s enzymes, primers, liquid media or any other solutions, particularly those that are sensitive to contamination, without permission! And even if you do get permission, then…

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Pimp Your Plasmid Growth Medium

…out there. I suppose rational scientists often have irrational superstitions. One example of an old method that could be improved is the growth media used for plasmid preparation. The majority…

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Tips for Successful Bone Marrow Isolation 

…differentiation media and on certain cell culture plates. Enrichment media contain cytokines (i.e. M-CSF) that stimulate the growth of specific cells. Using specific cell culture plates (tissue-culture treated vs. non-tissue-culture…

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How Do Buffers Work?

media that can support the growth of a lot of cells. The cells will secrete various chemicals as they grow, live, and die. This is just part of their metabolism…

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Crash Course in Microbial Identification

…species. These tests are probably more reliable in combination with catalase/oxidase testing and microscopic examination. These tests overlap with the concept of selective growth media. A wide range of selective media exists…

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