Search results for: social media
How to Get Started with Algae
…depending on the species. You will have to take this into consideration when starting your cultures. To optimize conditions, grow algae in conical flasks containing 100 mL of media. Media…
Read MoreHow to Overcome Minor Issues in Anchorage-Independent Assays
…problems can occur: the agar solidifies before mixing with the media the media won’t mix with the agar To avoid premature solidification of agar, pre-heat a beaker containing distilled water…
Read MoreThe Care and Keeping of Primary Murine B Cells
…all of your experiments. Make sure you make your media fresh. Media components, like glutamine, are critical for cellular growth and proliferation but can deteriorate quickly in the media. Alternatively,…
Read MoreFive Ways to Help You Achieve Happiness in Graduate School
…something that you love you boost your happiness levels and your productivity 4. Don’t neglect your personal and social life. Studies show that everyone feels happier when they socialize, including…
Read MoreKeeping Track of Your Literature
…tools, read our article on How to Speed Up Your Literature Searches by Crawling. Social media is also another very important tool for finding and keeping up with the latest…
Read MoreHow do you Solve a Problem like Pseudoscience?
…to all sorts of “information,” and knowledge is power. No subject or discipline is shielded from social media, and now more than ever the public is interested in the science…
Read MoreGo Fishing for RNA-Protein Interactions with a Yeast Three-Hybrid Assay
…a ADE2-mutant yeast strain, which allows you to check for RNA-dependent interactions. When levels of adenine in media are low, these yeast attempt to synthesise adenine but, due to a…
Read MoreAll sorted? Getting the best out of your cell sorting facility
…controls and samples but don’t forget your tubes or plates with media in them. It’s important that we sort into a liquid to prevent cell damage so bring some media…
Read MoreFeeder Cells – the What, Why and How
…[2] As inactivated feeder cells begin to die after 2-3 days, it is necessary to keep on replenishing the co-culture with more treated feeder cells and fresh media. Inactivating Feeder…
Read MoreThe Basics of NMR Part 1: Assessing Protein Folding by NMR
…rather than impart signal. Isotopically labeling a protein involves growing the host bacteria in yeast media or minimal media. These media allow you to control the nitrogen, carbon, or hydrogen…
Read MoreGet Your Proteins! Hot Proteins Here! Radioactively Labeled Proteins!
…swapping the normal culture media with culture media spiked with 35S-methionine. The labeled methionine is rapidly transported into the cells and is immediately incorporated into any newly-synthesizing proteins. At the…
Read MoreSubcellular Fractionation Protocols Explained
…physical properties using centrifuging in a high viscosity media. What kind of media you choose to use (e.g., sucrose, glycerol, or Percoll) depends on a number of factors, especially your…
Read MoreHow to Write a Press Release to Impress
…keep scientists from reaching out to the media and, by extension, the public. Sharing your science is beneficial to both the public and your own research. Being interviewed or writing…
Read More10 Useful Tips For Improving Your Sorting Experiments
…specific media, swap it out for a phenol red-free media for the sorting experiment and then collect them in the original media. 9. Plan ahead! Work out all the experimental…
Read MoreHow to Eliminate 99% of the Water from Your Culture, or Solid State Fermentation
When you think about culturing bacteria or fungi in large quantities, you likely envision flasks shaking or maybe bioreactors filled to the brim with liquid media. But did you know…
Read MoreFrom Cells to Scope: Chamber Slide Immunochemistry
…reagents than with standard ICC. A lot less. In a single-chambered slide, you can use up to several milliliters of media, but you can get away with as little as…
Read More10 Easy Ways to Wreck your Autoclave
…Not using secondary containment. Is your media bottle sitting directly on the rack or the chamber floor? When bottles burst (and sometimes they do), glass shards and molten media can…
Read MoreCulturing the Unculturable: Working with Difficult Bacteria
…simulation of the bacteria’s natural environment to encourage growth. Most standard culture media used in the laboratory are nutrient-rich and promote the growth of faster-growing bacteria, outcompeting slow-growing bacteria. Several…
Read MoreHow to Maintain Live Cells on a Microscope Stage
…also allow perfusion of different types of media. Full microscope enclosure: For dedicated live cell imaging systems, consider the full heated enclosure (if you have enough money!) that contains specimen,…
Read MoreWhat the HEK? A Beginner’s Guide to HEK293 CellsÂ
…serum-free media 293-H Fast growing; grow in suspension; grow in serum-free media; good adherance in plaque assays 293T High transfectability; contains the SV40 T-antigen 293FT Derivative of the 293-F cell…
Read MoreA Beginner’s Guide to Culturing Mouse Embryonic Stem Cells
…their media changing every day (you may see it turn from red to orange/yellow in only 24 hours). Share the burden with your colleagues. Everyone is owed at least one…
Read More“Viable But Non-Culturable (VBNC)”: Zombies of the Bacterial World
…and monitor the cultivability of the microorganism by counting the number of colony forming units (CFU) formed on culture media. If the microorganism is sensitive to the treatment, CFU numbers…
Read MoreHow to Use a Eukaryotic System for Protein Expression & Purification
…This is because insect cell media contains phosphate, rather than carbonate (which is present in mammalian cell media). When culturing insect cell lines, it is important to consider how frequently…
Read MorePractical Secrets of the Bacterial World for the Uninitiated
…different food niche markets (there are bacteria that eat nuclear waste!) and there is in turn a vast selection of growth media, each optimized to promote the growth of a…
Read More8 Basic Points to Remember Before Expressing Proteins in Bacterial Systems
…up different E.coli strains and plasmids is Ecoliwiki.net. Miscellaneous Factors: Media, Temperature & Induction The right media is important for optimal cell growth and division. You can test different media…
Read MoreTop 5 Tips for Maintaining Your Sanity During Thesis Writing
…the window when you’re thesis writing is your social life. Even if you have no responsibilities other than to get your thesis written and submitted on time, your social life…
Read MoreUnder Pressure: How to Stay Sane in a Publish-Or-Perish World
…from social media, perhaps on a regular basis. But that doesn’t mean withdrawing completely from social connection. Reach out to the friends you really want to speak to. Sometimes silence…
Read MoreThe 10 Unspoken Rules of Working in a Lab
…mean EVER use someone else’s enzymes, primers, liquid media or any other solutions, particularly those that are sensitive to contamination, without permission! And even if you do get permission, then…
Read MoreHot Tips for Creating a Scientific Special Interest Group at Your Institute
…event. Sheppard emphasizes that public events and network forums need to be advertised as widely as possible. Social media and mainstream media can be good tools for reaching out. When…
Read MorePimp Your Plasmid Growth Medium
…out there. I suppose rational scientists often have irrational superstitions. One example of an old method that could be improved is the growth media used for plasmid preparation. The majority…
Read MoreTips for Successful Bone Marrow IsolationÂ
…differentiation media and on certain cell culture plates. Enrichment media contain cytokines (i.e. M-CSF) that stimulate the growth of specific cells. Using specific cell culture plates (tissue-culture treated vs. non-tissue-culture…
Read MoreHow Do Buffers Work?
…media that can support the growth of a lot of cells. The cells will secrete various chemicals as they grow, live, and die. This is just part of their metabolism…
Read MoreCrash Course in Microbial Identification
…species. These tests are probably more reliable in combination with catalase/oxidase testing and microscopic examination. These tests overlap with the concept of selective growth media. A wide range of selective media exists…
Read MorePreparing Metaphase Spreads: The Breakdown on Broken-Down Cells
…harvesting! This almost works the same way as harvesting cell pellets, except you’ll retain the media with the colcemid, rather than pouring it away. Pour the colcemid-spiked media into a…
Read MoreKeeping Your Science Out of the (Junk) Headlines
…it is no doubt frustrating to see good science gone bad (or just bad science) perpetuated by the mainstream media catering to those with an interest in science at its…
Read MoreUsing Gage R&R Studies to Measure Precision in the Lab
…scientists are interested in supplementing media with an additive to determine whether it will significantly improve growth rates for your favorite cell line. To determine if the additive works, you…
Read MoreTissue Embedding and Sectioning: Something to Think About Whilst in the Bath.
In the same way that you should ‘Think Before You Fix’, the choice of embedding media should be dictated by your required end-point. The basic principle is that by processing…
Read MoreHomemade PCR Test for Mycoplasma Contamination
…when working with different cell lines, and aliquoting media) it is quite easy to test for mycoplasma contamination. While commercial test kits are quite easy and fast, they are often…
Read MoreIs Your Bacterial Culture Still Growing? A Primer on ODÂ600 Measurements
…media pH as a result of cell metabolism, or an accumulation of toxic cellular waste. Figure 1. Phases of a bacterial growth curve. Using OD600 Measurements to Determine Your Bacterial Culture…
Read MoreAvoid These Pitfalls: Seven (Not So Deadly) Histology Sins
…not #1.5 coverslips are the most common. This is because the thickness of the mounting media must also be taken into account when calculating optimal ‘coverslip thickness’. Therefore a #1…
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