You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.
By Heinz Reiske
Kits for DNA gel extraction are a great way to save time in the lab, but they are costly and produce much plastic waste. Discover three easy kit-free DNA gel extraction methods that can save you money and reduce waste in the lab.
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Let’s be honest—we scientists don’t always present our science in ways that are accessible to everyone. Nor is lab-based science always accessible. Explore what accessibility in science is and how we can all make science more accessible and inclusive.
Oil immersion microscopy can improve your resolution in microscopy. This article will explain why this is the case and how you can use oil immersion microscopy in the lab!
The Happy Scientist
#57 — Remember when you were a wide-eyed child and you dreamed of being the person who would invent some world-changing gizmo? You had lots of ideas back then. But now that you’re all grown up and worse yet, EDUCATED, have you run out of notions? Or have you found you have them but you…
#49 — In this episode, we talk to Elisabeth Bik, former microbiome researcher turned scientific integrity consultant. Elizabeth spends her time searching biomedical literature for inappropriately manipulated photographic images and plagiarized text, and often discusses these on Twitter at @MicrobiomDigest. Following her successful scientific career in the lab, a stolen sentence she authored led her…
#6 — Season 3. Sara De Biasi and Andrea Cossarizza from the University of Modena and Reggio Emilia (UNIMORE) chat with Peter O’Toole about how they were able to quickly respond to news of a novel coronavirus as early as January 2020, and how their work was crucial to understanding the effect of COVID-19 on…
#2 — Eva Nogales of UC Berkeley and Lawrence Berkeley National Laboratory uses cryoEM to study cellular processes related to cytoskeletal self-assembly and gene expression. In this episode of Cryo-Talk, Eva joins our host Eva Amsen to discuss the use of CryoEM to study complex cell biology systems and more. She chats about her current…