Latest Events
Illuminating Nanomaterial–Cell Interactions by Confocal Microscopy
Your Lab’s Sustainability Journey Starts in the Freezer
Take the burden out of buffer exchange with Unagi & Big Tuna
Effortless ELISAs: Streamline Assays with Walkaway Automation
Innovations and Emerging Tools in Peptide Science
Empower™ CDS Onboarding: How to Train New Users Effectively
On Demand Events
Latest Articles
DNA / RNA Manipulation and Analysis
When Correlation Fails: Why Transcriptome–Methylome Integration Can Fail (and How to Fix It)
Transcriptome–Methylome Integration often fails due to structural issues rather than biological absence. Key challenges include over-aggregation of CpG sites, variance mismatches, asymmetric data preprocessing, and inappropriate statistical models. Proper region mapping, variance assessment, covariate alignment, and cohort size evaluation are essential to detect true regulatory relationships. Addressing these factors before complex modeling improves interpretation and avoids false conclusions about methylation-expression associations.
Latest Podcasts
Ever re-run the same experiment three times just to keep getting “bad” results? In reality, no result is “bad” and experiments never “fail”. Instead, they produce data; sometimes that data is clear, sometimes it is not. After running an experiment, your results are either conclusive or inconclusive, and an inconclusive result is not useless. If…
Midpoint CETSA is a common method for assessing target engagement by measuring protein stability at a single temperature, but it can obscure important mechanistic differences in complex proteins. This article explains how multi-temperature CETSA profiles reveal conformational state-specific binding, improving compound ranking and selectivity assessment. It highlights when to move beyond midpoint CETSA, especially for covalent mechanisms, mutant selectivity, and structurally diverse compounds, providing a practical decision framework for drug discovery.
Choosing the right filter plate starts with understanding your assay biology. What must happen at the membrane determines everything that follows. Choose filter plates according to membrane chemistry (binding vs non-binding) and the correct pore size for your target (cells, proteins, or small molecules). Finally, ensure plate architecture and automation compatibility support consistent flow, minimal crosstalk, and reproducible results at scale.
qPCR Troubleshooting involves identifying and resolving common issues such as contamination, pipetting variability, reagent integrity, and reaction setup errors. This guide provides a clear framework to diagnose symptoms, optimize conditions systematically, and ensure reliable, reproducible qPCR results. It emphasizes the importance of proper primer handling, cycling conditions, and normalization for trustworthy data interpretation.
Microscopy & Imaging
Optical microscopy has always been restricted by the diffraction of light. Because conventional widefield and confocal microscopes cannot resolve structures substantially smaller than about 200nm laterally, they miss critical signaling compartments and nanoscopic organization. Newer super-resolution technologies, such as stimulated emission depletion (STED), structured illumination microscopy (SIM), and photoactivated localization microscopy (PALM), solve this problem…
DNA / RNA Manipulation & Analysis
Transcriptome–Methylome Integration often fails due to structural issues rather than biological absence. Key challenges include over-aggregation of CpG sites, variance mismatches, asymmetric data preprocessing, and inappropriate statistical models. Proper region mapping, variance assessment, covariate alignment, and cohort size evaluation are essential to detect true regulatory relationships. Addressing these factors before complex modeling improves interpretation and avoids false conclusions about methylation-expression associations.
How-To Guides
Article Categories
- Analytical Chemistry & Chromatography Techniques
- Cells & Model Organisms
- Chemistry for Biologists
- Cloning & Expression
- DNA / RNA Manipulation & Analysis
- Flow Cytometry
- Genomics & Epigenetics
- Microscopy & Imaging
- PCR, qPCR & qRT-PCR
- Bioscience Mastery
- Basic Lab Skills & Know-how
- Career Development & Networking
- Dealing with Fellow Scientists
- Equipment Mastery & Hacks
- Getting Funded
- Lab Safety
- Lab Statistics & Math
- Organization & Productivity
