Latest Events
Your Lab’s Sustainability Journey Starts in the Freezer
Take the burden out of buffer exchange with Unagi & Big Tuna
Effortless ELISAs: Streamline Assays with Walkaway Automation
Innovations and Emerging Tools in Peptide Science
Empower™ CDS Onboarding: How to Train New Users Effectively
Streamlined High Pressure Freezing for Cryo-ET: Waffle Method to Serial Lift-Out
On Demand Events
Latest Articles
Career Development and Networking
How to Make a Sector Shift Confidently Using a “Career Experiment”
Navigating bioscience careers involves defining clear questions, identifying knowledge gaps, and testing assumptions before major changes. Understanding sector-specific challenges and leveraging mentorship can reduce uncertainty and support informed decisions. Recognizing the difference between productive stretch and overwhelming panic zones helps manage transitions effectively. This approach fosters adaptability and resilience, ensuring career shifts are deliberate and aligned with personal and professional goals.
Latest Podcasts
Geobacillus stearothermophilus is a thermophilic bacterium whose highly resistant spores are used to test autoclave sterilization efficiency. Its spores survive extreme heat due to molecular defenses, making them ideal biological indicators. Proper autoclave validation requires biological indicators rather than relying solely on gauge readings or chemical indicators to ensure complete sterilization in medical and laboratory settings.
Transcriptome–Methylome Integration often fails due to structural issues rather than biological absence. Key challenges include over-aggregation of CpG sites, variance mismatches, asymmetric data preprocessing, and inappropriate statistical models. Proper region mapping, variance assessment, covariate alignment, and cohort size evaluation are essential to detect true regulatory relationships. Addressing these factors before complex modeling improves interpretation and avoids false conclusions about methylation-expression associations.
Ever re-run the same experiment three times just to keep getting “bad” results? In reality, no result is “bad” and experiments never “fail”. Instead, they produce data; sometimes that data is clear, sometimes it is not. After running an experiment, your results are either conclusive or inconclusive, and an inconclusive result is not useless. If…
Midpoint CETSA is a common method for assessing target engagement by measuring protein stability at a single temperature, but it can obscure important mechanistic differences in complex proteins. This article explains how multi-temperature CETSA profiles reveal conformational state-specific binding, improving compound ranking and selectivity assessment. It highlights when to move beyond midpoint CETSA, especially for covalent mechanisms, mutant selectivity, and structurally diverse compounds, providing a practical decision framework for drug discovery.
Microscopy & Imaging
Optical microscopy has always been restricted by the diffraction of light. Because conventional widefield and confocal microscopes cannot resolve structures substantially smaller than about 200nm laterally, they miss critical signaling compartments and nanoscopic organization. Newer super-resolution technologies, such as stimulated emission depletion (STED), structured illumination microscopy (SIM), and photoactivated localization microscopy (PALM), solve this problem…
DNA / RNA Manipulation & Analysis
Transcriptome–Methylome Integration often fails due to structural issues rather than biological absence. Key challenges include over-aggregation of CpG sites, variance mismatches, asymmetric data preprocessing, and inappropriate statistical models. Proper region mapping, variance assessment, covariate alignment, and cohort size evaluation are essential to detect true regulatory relationships. Addressing these factors before complex modeling improves interpretation and avoids false conclusions about methylation-expression associations.
How-To Guides
Article Categories
- Analytical Chemistry & Chromatography Techniques
- Cells & Model Organisms
- Chemistry for Biologists
- Cloning & Expression
- DNA / RNA Manipulation & Analysis
- Flow Cytometry
- Genomics & Epigenetics
- Microscopy & Imaging
- PCR, qPCR & qRT-PCR
- Bioscience Mastery
- Basic Lab Skills & Know-how
- Career Development & Networking
- Dealing with Fellow Scientists
- Equipment Mastery & Hacks
- Getting Funded
- Lab Safety
- Lab Statistics & Math
- Organization & Productivity
