Multiplex CRISPR Gene Editing: What’s It For?
The RNA-guided nature of CRISPR nucleases (e.g., Cas9, Cpf1) makes them highly amenable to multiplex applications, including multi-gene knockout and large-scale genomic modifications. This article — part 1 of a two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to CRISPR gene…
By Patrick Miller-Rhodes
How to Read a Scientific Paper Efficiently
Keeping up with the scientific literature in your field of interest is incredibly important. Not only does it keep you informed as to what your…
Nodulation: What Is It and Why Is It Important?
Plants are incredibly organisms. Not only do they provide atmospheric oxygen, but, in the case of legumes, they can transform atmospheric nitrogen gas to ammonia,…
What the HEK? A Beginner’s Guide to HEK293 Cells
If you’re at all familiar with cell culture, you’ve probably heard of HEK293 cells. HEK293 are one of the most commonly used cell lines in…
How to Share Your Lab Protocols and Why It Benefits You
Reproducibility is a cornerstone of scientific research and your results need to be reproducible not only by yourself but also by others, both in and…
Tips on Restriction Digests: Designing a Restriction Screen
Restriction digests are the cornerstone of many techniques. Here are some great tips on setting up and troubleshooting them.
