Blunt and sticky might sound dull and dirty but knowing how these different cloning methods work is important when choosing which method to use. Here we give you a guide to how blunt-end cloning works. Blunt-end cloning is the cloning of DNA fragments containing no unpaired bases at the 5’ and 3’ prime ends (i.e.…

Antibiotics are used in a wide range of techniques in molecular biology including molecular cloning and are important for treating pesky mycoplasma contamination in cell cultures. They can also be used to maximize your plasmid yeilds by reducing protein synthesis, in certain circumstances. The aim with this post is to provide an easy reference to…

The physical arrangement of a laboratory can have a strong effect on a bioscientist’s efficiency; those extra steps and seconds of time can really add up! Here is a compilation of tips to help you organize your lab space to minimize inefficiencies and maximize your productivity in the lab. Organize Your Lab Space with Stations…