Latest Events
Winning Westerns: Tips and Tricks for the Perfect Western Blot
Going with the Flow: Your Ultimate Guide to Ultrafiltration
Biosynthesis of Natural and New-to-Nature Glycosides
Does Neural Activity Shape the Developing Brain Vasculature?
Automating and Optimizing Cell Counting (With No Plastic Slides)
Scientific Rigor Meet-up: How Reliable Reagents Build Reproducible Data
Seeing the Complete Cell: From Confocal Microscopy to Volume EM and Cryo-EM
Precision PCR Meet-up: Gene Expression, Validation, and Development
Latest Articles
Patient-derived Organoids for Oncology Drug Development: What You Need to Know
Many oncology drug candidates fail in the clinic, partly because screening models don’t capture the biology and heterogeneity of real tumors. Patient-derived organoids offer a middle ground: they’re more disease-relevant than cell lines, and faster and more scalable than xenografts. Find out how to get the most out of them in your drug screen.
Brought to you by a “Very Cool Guy” at a conference, it seems that every scientific technique follows the same path to obscurity. This light-hearted guide walks you through the 8 stages of a method’s life cycle, then looks back at a number of historical techniques to see which ones actually faded away for good and which ones are still about today.
Need a big plasmid prep? These tips help you maximize plasmid yield without overgrowing your culture. Learn why starting from a fresh colony matters, how aeration and harvest timing affect quality, why gentle mixing protects your prep, and the ethanol-wash mistake that sent one technician’s plasmid down the drain.
AI in science can draft your discussion section, summarise forty papers and even propose novel hypotheses. So are you becoming less essential to your own work? The real risk to your job isn’t replacement, but letting polished output go unexamined. Here’s the one question that keeps your judgment, expertise and ownership firmly yours.
Suicide PCR was built for one purpose: stopping PCR carryover contamination from faking a positive in ancient DNA work. It identified Yersinia pestis in medieval plague burials and still teaches a useful lesson about experimental design. But is it a technique you would reach for today? Here’s our take on whether it’s still a useful methodology.
Microscopy & Imaging
Optical microscopy has always been restricted by the diffraction of light. Because conventional widefield and confocal microscopes cannot resolve structures substantially smaller than about 200nm laterally, they miss critical signaling compartments and nanoscopic organization. Newer super-resolution technologies, such as stimulated emission depletion (STED), structured illumination microscopy (SIM), and photoactivated localization microscopy (PALM), solve this problem…
DNA / RNA Manipulation & Analysis
Need a big plasmid prep? These tips help you maximize plasmid yield without overgrowing your culture. Learn why starting from a fresh colony matters, how aeration and harvest timing affect quality, why gentle mixing protects your prep, and the ethanol-wash mistake that sent one technician’s plasmid down the drain.
How-To Guides
Article Categories
- Analytical Chemistry & Chromatography Techniques
- Cells & Model Organisms
- Chemistry for Biologists
- Cloning & Expression
- DNA / RNA Manipulation & Analysis
- Flow Cytometry
- Genomics & Epigenetics
- Microscopy & Imaging
- PCR, qPCR & qRT-PCR
- Bioscience Mastery
- Basic Lab Skills & Know-how
- Career Development & Networking
- Dealing with Fellow Scientists
- Equipment Mastery & Hacks
- Getting Funded
- Lab Safety
- Lab Statistics & Math
- Organization & Productivity
