In this article I will not talk about ‘wild’ proteases, which destroy cellular proteins in your lysates like wolves destroy sheep. Instead, I’ll be talking about the shepherd dog proteases—purified, tame and useful to digest proteins your research. In Protein Research and Crystallization Several programs can predict your protein domains. However, we wet biologists know […]
After the initial excitement of growing and isolating plasmid DNA, routinely preparing plasmid mini/midi/maxi preps gets boring. You definitely want a way to squeeze the maximum amount of plasmid DNA out of your culture. Good news—you can increase your plasmid yield using antibiotics. Keep the Pressure On to Improve Plasmid Yield Remember that you need […]
If there is one profession that benefited from globalization, it is the medical writer. While the university research groups shrink and global biomedical companies fire their research stuff, medical writing companies are expanding, providing stable jobs with good salaries. The American Medical Writers Association (AMWA) reported in 2011 that the median salary of an experienced […]
Ideally, your tissue culture incubator should be polished stainless steel, gleaming and immaculate like a surgical theatre. And I am sure you keep it in order, like new. It’s just sometimes you start in a lab where the incubators already have brown spots – rust. There’s Rust in My Incubator! Usually rust occurs because of […]
When it comes to registering the signal output of your Southern/Northern/Western/probe hybridization, you are spoilt for choice these days. You can go all retro and use X-ray film. You can go digital and use a phosphorimager. Finally, you can go fluorescent and use a fluorescence detector. So, what are the pros and cons of each […]
Most eukaryotic proteins exist as several isoforms, differing in posttranslational modifications, which allows them to perform slightly different functions or the same function under slightly different conditions. A common posttranslational modification of proteins is glycosylation.
A scientist’s life is full of stress. An experiment is not working— stress, experiment working but producing results opposite to the previous one— stress, somebody using the last of the reagent you need now— more stress. But these are unexpected stresses, small and overcome easily. The ‘planned stresses’ such as meetings with your supervisor or […]
I’ve never run a sequencing gel in my life, but people around me did, and they spent a lot of time on getting it just right. Although the principle described by Sanger in 1975 sounds straightforward (1), sequencing gels are very long and very thin – less than a millimeter thick! They were easy to […]