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Can You Stand the Cold? Cryosectioning for Beginners

Before you can perform histology on your tissue samples – you need to prep them. This means you must fix them, embed them and section them into thin slices for analysis. A great way to slice your tissues is...
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Article

Outsourcing Research: Should Your Experiment Spend Some Time Away from You?

As a researcher, it’s satisfying to manage your own projects and do the bench work yourself. After all, if you don’t have experience with a technique, you’re usually expected to figure it out (with or...
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Article

World of Microbes Part 4: Food and Biofuels.

In this World of Microbes series, so far we’ve covered the importance of microbes in medicine including their use in vaccines, antibiotics, probiotics and protein production of antibodies for immunotherapy....
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Article

A New Frontier in Protein Quantitation: AlphaLISA

If you ever worked in a biology or biochemistry laboratory, you probably already heard about ELISA. You may have even used it. But do you know what’s behind it? And how you can improve it? Let me guide you...
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Article

Be More Objective in Your Approach to Science

Part of the fun of science is the opportunity to conjure up hypotheses and ways in which to test them. Of course, being scientists, our ideas are highly thought out and logical. We aim to test the hypothesis...
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Article

Mastering the Art of Growing THP-1 cells

Tissue culture can sometimes seem like a black art. Too careful—your cells go down. Not careful enough—your cells go down. A butterfly flutters its wings in the middle of the Atlantic Ocean—your cells go...
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Article

Five Tips to Increase Your Lab Efficiency

Starting a PhD program is both an exciting time as well and a challenging one... One of the main things that keeps most PhD students up nights, is rethinking their steps in the lab during the day.  Did I do...
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Article

Hydrodynamic Focusing in Flow Cytometry

If you have sorted samples or phenotyped cells by surface expression of proteins, you've probably wondered how each cell is sorted or phenotyped in a flow cytometer? This question seems trivial, but in reality...
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Article

Viral Vector Production: Myths & Misconceptions

Viral vector production is a worthwhile skill that can be made even easier with a few tips and tricks. In general, transfection of multiple plasmids into a producer cell line results in infectious,...
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Article

How to Scrutinize Your Glycosylated Proteins Without Using Glycosidases

You might have come across protein glycosylation before. Somewhere in the recesses of your memory you might even recall reading something about the protein you’re studying being glycosylated, but what does...
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