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Hierarchical or Boolean Gating: Which One to Choose?

A flow cytometer collects the events you are interested in, and also ‘sees’ every event that goes through. This includes debris and even bits in your buffers. As cytometrists, we gate our cells to exclude...
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Article

Advocacy for Scientists – Why and How

Advocating for the research that scientists perform is important.  Your advocacy helps politicians and the general public understand why funding is needed. In fact, many funding agencies require a disease to...
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Article

How DNA Extraction Is Different in Plants

I know what you are thinking, everything is made of cells, so how different can DNA extractions be in plants? The answer is… sort-of different. The overall concept is the same. Cell membranes are lysed, DNA...
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Article

How to Generate High-Quality SNPs Data Sets from NGS

SNPs or single nucleotide polymorphisms are on many scientist’s wish list in experimental studies of genomic DNA sequences. Methods to detect SNPs have evolved. Now with the availability of high throughput...
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Sponsored Education

Size-Selection Is Essential for Cell-Free DNA Studies

Advances in using cell-free DNA (cfDNA) to glean clinically meaningful information for a patient have been stunning. For the most part, these research studies (or downstream diagnostic tests) isolate fetal DNA...
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Article

Basic Bacterial Culturing Practices

Mastering basic bacterial culturing practices is a must if you are planning a career in microbiology! Growing bacteria might be one of the easiest things to do as a scientist. Also, as you’ve probably...
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Article

Water your choices? Understanding Types of Water in the Lab

If you are working in a scientific laboratory, it is very important to be aware of the various types of water available, because the purity may not be acceptable for your specific experimental...
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Article

Investigating an Expression Quantitative Trait Locus (eQTL)

Thousands upon thousands of genetic variants are now associated with every disease and trait you can possibly think of. Such traits range from cancers to blood pressure, intelligence, height, weight… and many...
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Article

Prioritize Your Way to Success: a 4-Step Guide

So you just got out of your adviser’s office, feeling inspired, you get to your desk and look at the 27 new things added to your never-ending to-do list. You may feel overwhelmed, like you will never get all...
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Article

Running your Lab on a Shoestring Budget

We live in challenging times for academic biomedical research. The success rates for research grants have declined precipitously over the years. Under these circumstances, it is never too soon to take proactive...
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