" The Missing Manual For Bioscientists "
Bubbles isn’t just the name of my favorite cartoon character from Power Puff girls, or just the best activity for a kid to play with, in general. In my adult world, they stand for a whole lot more, but can still cause extreme emotions. At the lab bench, seeing bubbles brings happiness or sadness depending…Read More
Biosensor chip selection is a critical step in planning and running a surface plasmon resonance (SPR) experiment. Chip selection depends on the ligand or target that needs to be immobilized on the sensor chip, the analyte that is flowed over the target to study the binding, and the purpose of the biosensor assay (i.e., determination…Read More
The polymerase chain reaction (PCR) is the backbone of many lab techniques. In short, it allows for the exponential amplification of a specific segment of DNA. Through the use of primers encoding restriction enzyme sites, these amplified fragments are used in downstream cloning procedures, usually leading to the insertion of one, maybe two, PCR fragments…Read More
Simple Tips for Model Organism-Based Work The mouse is the favored model organism for life science researchers so much so that mice account for about 95% of all lab animals used in research. The striking similarities between the human and mouse genomes, ease of genetic manipulation and the uniformity achieved through inbred mating makes them…Read More
Apoptosis, or programmed cell death, is a physiological process in which individual cells are set to die without harming their environment. It involves a cascade of complex and tightly regulated cellular events. Detecting apoptosis on tissue slides, involves either detecting the molecular participants of these events, or the morphological changes that occur on the cellular…Read More
In biology, a molecular barcode is a characteristic DNA sequence used to distinguish and gather together similar items. Such a simple but powerful concept is useful in various applications. As an example, the Barcoding of Life project aims to identify specimens through the sequencing of standard gene regions, and use these as barcodes. On the other…Read More
Every bio- scientist who wants to analyze DNA knows that the process begins with the extraction of DNA from cells of interest. These cells could be RBCs, parasites, or bacteria to name a few. Furthermore, there are various DNA extraction methods1 to choose from depending on sample type, downstream analysis, and so forth. Many scientists…Read More
My first lab experience was in a lab on a really strict budget: no kits, no technicians, no media kitchen, or glassware washing service. We really had only minimal resources. I was close to paranoid about not wasting one tip or glove. Later, when I started in other labs, I spent my first days going…Read More
You have your favorite protein in mind and are ready to set up some exciting experiments to show what it does and how it does it, when it is active, what other proteins it modifies, and how it affects your cells. There is one slight problem. You need to fish your favorite protein out from the…Read More
Do you wonder if your favorite protein is entwined in a delicate dance with another protein? Do you wish that you could shine a spotlight on your protein to determine its dance partner? Well good news! While there is no such spotlight, you CAN use co-immunoprecipitation (Co-IP) to find your protein’s dance partner. You can…Read More
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