" The Missing Manual For Bioscientists "

Codon Optimization for Increased Protein Expression

By GenScript | June 23, 2017

The Genetic Code: A Universal Template for Protein Translation All known organisms share the ‘central dogma’ of molecular biology. DNA is transcribed into mRNA that is translated into protein. During the discovery of the genetic code, Francis Crick hypothesized that translation required a mediator to aid mRNA-guided translation according to a number of specifics. Amongst…

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Antibody Validation

By GenScript | June 22, 2017

Not all antibodies are valid for every experiment and condition, and they must be validated for the specific application and species. Currently, there is no standard means of “antibody validation,” and this can greatly impact experimental reproducibility and reliability. Journals and granting agencies have taken steps to address this gap. Many now require you to…

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How to Use CellProfiler for Cell Imaging

By Khushbu | June 22, 2017

Are you trying to figure out how to calculate intensities of fluorescently-labeled single cells? Do you have cells at high densities or present in clusters? Are you worried that your current cell imaging analysis software is unable to mark clear boundaries around each cell in a cell cluster? Don’t fear, because CellProfiler 2.1 is here to…

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Cre-loxP Recombination Essentials Part 2

By Sweena Chaudhari | June 21, 2017

The Cre-loxP recombination system is routinely used for the generation of mouse knockouts. In part 1 of this mini-series, I introduced the concept and applications of Cre-loxP. As with any other technology or research tool, it has limitations and pitfalls that need to be considered while planning experiments or interpreting results. This article will take…

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How to Write a Killer Research Interest Statement

By Serena Quarta, PhD | June 21, 2017

Before you know it, the day will come when your contract ends, or you just feel like applying for a new and exciting position. You may feel a bit lost in preparing all the documents you need for that new job post. Do not worry, there is advice out there. One thing you will need…

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A Crash Course in CRISPR-Cas9 Editing in Drosophila

By Chiu-An Lo | June 20, 2017

CRISPR-Cas9 has become a magic tool for molecular biologists, transforming genetic engineering from a once unbelievable dream into tangible reality. Today, you can easily edit primary cells or cell lines within a few weeks with well-established protocols or others’ hands-on advice. However, no matter how many success stories you find online or hear at seminars…

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Introduction to DREADDs – Control Over G Protein Coupled Receptor GPCR signaling

By Christina Lebonville | June 20, 2017

Gee, Protein, What Do You Do? Manipulation of a system under investigation is the backbone of experimentation. A new tool called Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) allows us to hijack cell signaling and study cell function within living organisms. Like its cousin technique, optogenetics, DREADD technology uses a viral vector to introduce…

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How to Design a CRISPR Experiment and Start Genome Editing

By GenScript | June 19, 2017

CRISPR and the CRISPR Associated system (Cas) are powerful gene editing technologies. Originally identified and characterized in bacteria, the endogenous CRISPR systems act as an RNA-based defense mechanism against invading phage DNA. CRISPR cas9 gene editing was adapted for genome editing in 2013 and has since been exploited for its ability to generate targeted double-stranded DNA…

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Networking – You Know It’s a Thing

By Ruth Barrett | June 19, 2017

A supportive network is important for your mental health and happiness. This is particularly true during stressful times, like grad school and career transitions. Networking – meeting people in the science community is important for professional development and meeting people outside of science is good for balance. At every transition after grad school, you start…

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How to avoid accidental plagiarism and academic fraud [BMA Podcast]

By Dr Nick Oswald | June 16, 2017

In the modern research environment we have all of the information we need to work with right at our fingertips – just a cut and paste away. And that makes it very easy for even the most well-meaning scientist to accidentally stumble into plagiarism. Most of us think of plagiarism as simply copying someone else’s…

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