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Proteomics and Good Mass Spectrometry Data

It is currently possible to analyze thousands of proteins in a single sample using mass spectrometry (MS) and a database of predicted protein sequences, referred to as ‘bottom-up’ proteomics. With this...
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Article

Four Ways to Get CRISPR Reagents Into Your Cells

Do you need to test the effects of mutating a gene in your system?  Then, CRISPR is the way to go. Your first step is to decide on good target sequences. Then, you have to get the two components of CRISPR: the...
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Article

Benefits of the GUS Gene Reporter System in Plants

Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS,  is short for glucuronidase, an enzyme in the bacterium...
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Automated Cell Counting with a Fluorescent Twist

Cell counting is the bane of existence of many researchers. Countless hours spent in front of the microscope with a haemocytometer on the stand and a manual tally (or “clicker”) in hand can be really...
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Are You any Less of a Scientist after You Transition to Non-Bench Science?: Opinion

In this ever-evolving world, scientists in “alternative”, non-academic positions are more commonplace than ever. Gone are the days where ideas of leaving bench science would label you as a “sell-out”....
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Phylogenetic Tree Construction Made Easy with Blast & Mega

Your DNA sequence can be put to good use fairly easily with Blast and Mega software. These programs can help in phylogenetic tree construction. You can ask questions like what is the evolutionary relationship...
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Article

Breezing Through the Aftermath of a Conflict

When working very closely with a team of people in the lab, you will have disagreements leading to conflict. That’s just human nature, hence the origin of a common phrase, “Can’t we all just get...
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Small Particles (Things) Matter!- Introducing Nanoparticle PCR

There are many different methods and protocols on making your PCR  run more efficiently. I recently came across an interesting PCR method called “nanoparticle” PCR. This method seems to attract a lot of...
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Article

Analyze Immunostained Slides with Semiquantitative Scoring

A  routine task in the lab is to investigate the presence of your favorite protein in a range of histological samples. No doubt, staining your tissue sections using good old immunohistochemistry (IHC) would be...
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Article

Murphy’s Law Lives in my Lab

Some days are good and things run like clockwork. Then there are those other days - you know the ones - where you mutter to yourself, “Can’t anything go right today?!?!?” Those are the days you are...
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