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Setting up a Fermentation or Perpetuum Mobile Cell Culture

Some names are confusing. For example, ant-lion is not an ant - or a lion. Likewise, fermentation in the scientific sense does not involve using a ferment or brewing beer. In science, fermentation is the...
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Quantifying & Assessing RNA: TE or not TE?

Red Pill or Blue? Carrying out science often involves many difficult decisions! I see it all the time in RNA protocols - the “gracious” option of using purified water or Tris-EDTA (TE) buffer to dissolve...
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Article

How to Give an Incredible Scientific Talk

Committee meeting approaching? 10 minute department seminar? Lab meeting? Fear not! My adviser has insulted my presentations so regularly, that I’ve finally learned some things. Hopefully, I can head your...
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Article

Divide and Conquer: How to Setup Your First Droplet Digital PCR Experiment

Droplet digital PCR?  It's easy. Because we’re here to guide you through it. We recently introduced you to the principles of digital PCR technology and how it differs from qPCR. In a nutshell, digital PCR...
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Article

Sample Preparation for Scanning Electron Microscopy

Proper sample preparation plays an important role in obtaining the required information when using scanning electron microscopy (SEM). You need to consider the sample’s size, shape, state, and conductive...
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How to Choose the Correct Reverse Transcription Method

Quantitative Reverse transcription PCR (RT-qPCR) is frequently used in the lab to detect and quantify RNA expression in a sample. The first step of the assay is to convert the labile RNA to its complementary...
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Article

A Primer on Checking the Methylation State of the Genome

We all know that genes encode proteins that make up a living cell. However, the level and coordination of gene expression is really the key to the success of a living cell. One way eukaryotic cells (that’s...
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Article

Coping with Bereavement as a PhD Student

Imagine being a PhD student whose life mostly revolves around the lab until you are hit by the worst news of your life. You have just lost one or both of your parents and bereavement sets in. You still cannot...
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Article

How to Focus When Your Colleagues Are Wreaking Havoc Around You

Now, we all love our colleagues – true, some are more loveable than others, but still. However, sometimes they can be very noisy. Especially when you need to focus.  For example, when you are in the middle...
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Article

Molecular Docking: Let the Docking Do the Talking

Want to know whether a lead molecule or a ligand of your choice interacts with a particular protein or a receptor? Do you want this information at your fingertips? All it takes is just a few clicks and key...
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