To Pour or to Spread, This is the Question

Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio   It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates […]

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Crap in, crap out: Flushing Out The Problems in Your Flow Cytometry Data

“What Have You Done To My Cells??!!!” This cry of pain from researchers, frequently aimed at core facility operators, is heard after receiving incomprehensible data for an invaluable tube of cells. Equally baffling to […]

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All for one and one for all – Fluorescence Minus One Controls

Ever done a multicolour flow cytometry experiment, run all your controls, done your compensation and then started to analyse your data and realised that you can’t work out where to put your gates? To […]

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One For You, One For Me… How a Cell Sorter Works

Cell sorters do not operate by magic, even it looks that way. It’s about the application of physics, electronics, fast computers and formation of droplets. Whether you bring your cells to a flow core […]

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The Lac Operon Explained

An operon is a functioning unit of genomic DNA that contains a group of genes controlled by a single promoter. Put simply, these genes share information needed to create the tools for a particular […]

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Heating up agar? Just add a cup of water and avoid the glitter and crumbs

It’s ironic how much folklore and superstition comes with being in science. “That’s a lucky pipette”, “playing Bach for your cells will help them grow”, “always make your own solutions”; we all have our […]

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Stereo (Dissecting) Microscopes 101

  Think a stereo microscope might be helpful? But you don’t know how to use one? Lucky for you I wrote this primer on stereo microscopes to help you figure out when and how […]

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Have I Got Good Protein Expression News for You

While the art of reading hand poured sequencing gels is forgotten as the next generation sequencers spit out genome after genome, protein expression still often requires sweat, tears and a lot of luck. I […]

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Taken for Granted: Position and Setup of Your Microscope

Although I say ‘taken for granted’, over the years of working in and managing microscope facilities, it quickly became clear that the position of the microscope, user position and set-up were aspects which were […]

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The History of PCR

As with some of the greatest discoveries in science, from penicillin to microwave ovens and play-doh, PCR was discovered serendipitously. Thanks to the work of many scientists, including Watson and Crick, Kornberg, Khorana, Klenow, […]

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