Say Goodbye to Restriction Enzymes and Ligases: An Introduction to Sequence and Ligase Independent Cloning (SLIC)

SLIC, or sequence and ligase independent cloning, was developed by Li in 2007 and published in Nature Methods. What makes it a Nature Methods worthy protocol? Unlike other forms of cloning, SLIC does not require restriction enzymes or a ligase! Seriously! Don’t believe […]

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Top Ten websites to help you with your PCR experiments

Whether you are embarking on a PhD in molecular biology, trying to amplify a new gene, analyzing gene expression by qPCR, or trying to solve a PCR problem, you will probably resort to a […]

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Making The Most Out Of Your Commute To The Lab

Power nap anyone? Depending on how long your commute is, and what type of transport you use, you could make your commute useful. If you are taking public transport, you can use that time […]

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The Nature of Denaturing (Protein Gels, that is!)

You've nurtured your cells for weeks, perfected your experimental conditions, and nailed down all the controls. You've harvested your cells and gently lysed them, now you're ready to look at the proteins. What's one […]

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The power of STED microscopy, Part 1: How does it work?

Do you suspect that your favourite protein is doing something really cool? But you cannot see it because your confocal microscope’s resolution is limited. Then Stimulated Emission Depletion (STED) microscopy is what you need! […]

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Fixation and flow cytometry

Fixation is routinely used in histology and cytology Labs the world over as a way of keeping cells in stasis at a particular point to ensure that, by the time they are examined, they […]

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Common Myths of Copyright

Copyright is something that a lot of scientists only give a passing thought to. However, this is something that affects us all. If you publish your work, then you need to understand copyright, the […]

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Deciphering your Materials Safety Data Sheet

For all the chemical reagents that we may use on a daily basis, there are many for which we still need to learn how they work and what they can do. Thankfully, for a […]

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The Dos and Don'ts of Weighing Dangerous Chemicals

A lot of chemical reagents are relatively nonhazardous. But there are just as many that are extremely hazardous, which means you’ll want to take precautions to reduce any risk of exposure, repeated exposure and […]

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Taking the Pain, Challenge and Rage out of PCR: The BitesizeBio Guide to PCR

Polymerase Chain Reaction, better known simply as PCR, has come a long way in the past 30 years. For those of you old enough to remember the not so ‘good old days’ when PCR […]

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