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5 DNA Ligation Tips

DNA ligations can be frustrating. Sometimes they just don't work, for no obvious reason. My top 5 DNA ligation tips will help to improve the efficiency of your ligation reactions and hopefully give a better...
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Article

Qualifications for Off-the-Bench Science Careers

Scientific research is a demanding and stressful career requiring lots of patience, dedication and hard work. Sadly the monetary benefits are not commensurate with the effort. Moreover, lack of opportunities,...
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Article

No More White Elephants! – Consider this Before Buying a Real-time PCR Cycler

Does your lab have a closet full of white elephants; once expensive instruments that are no longer fit for purpose, or have broken down? In many cases, all of that wasted money and resource could have been...
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Article

How to Switch PhD Advisors

You’ll know when it’s time to go. Maybe it was when she changed your project for the sixth time in six months, though your thesis proposal deadline was rapidly approaching and you had nothing to present. Or...
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Article

Why Do Enzymes Have Optimal Temperatures?

Every biologist is familiar with the profile of the rate of an enzymatic reaction versus temperature as shown in the figure. We know that enzymes from E. coli or warm-blooded animals tend to have an optimum...
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Article

How to Get Started with Algae

Do you know about algae and their potential in today’s world? Do you know how to work with algae? Algae are becoming increasingly important in the research world.
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Article

Expert Tips on Managing Your Data

After ten years of postdoctoral research there is one important piece of advice I would give to anyone embarking on a research career: Spend as much time managing your data as you do generating it Take...
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Article

Get that Genotyping PCR to Work EVERY TIME

Say you just joined a lab and have been assigned your very own project to work on. As part of your new responsibilities, you have to breed and maintain the mutant (or transgenic) mouse line which you will be...
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Article

Survival as a PhD Student: Keep the Post Docs on Your Side

From one lowly PhD student to another: we need post doctoral scientists. From their ability to seemingly do everything right to their moral support after a weekend of failed experiments and questioning the...
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Article

MIFlowCyt Guidelines: Helping You to Publish Your Flow Data

Wow, you’ve done it! Your experiment worked and your boss asked you to write it up for publication in your favorite journal. Where to start with presenting your flow data? Take a deep breath, help is in hand...
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