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Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication

What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even possible? In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make this possible. We will discuss how to […]

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In Microscopy & Imaging 29th of March, 2017 Presented by: Frédéric Leroux, Shigeki Watanabe
Article

Show Disparity in Gene Expression with a Heat Map

Have RNA-seq or microarray data? What possible tools can help you find your genes of interest? Is there any pattern in your expression data? I know you are totally at sea but heat maps are now commonly used to...
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Article

Are Quantum Dots Any Good for Flow Cytometry?

What Are Quantum Dots? Quantum dots were discovered in the early 1980s. However, it was not until the late 1990s that their use in biological applications was suggested.1 Quantum dots are semiconducting...
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Article

Five Reasons Why You Should Have Hobbies While in Graduate School

You’ve chosen a career in science and gone off to graduate school because you love doing it. For you, lab is home. You are so used to wearing your lab coat all the time that when you go to the kitchen to boil...
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Article

In Situ Zymography: Let’s Catch that Enzyme in Action!

In situ zymography (ISZ) is the best choice to study proteases. Proteases are a challenge to study as proteases are extremely potent enzymes. As such, they need to be controlled at multiple levels to prevent...
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Article

Should I Stay or Should I Go? Part II: How to Gracefully Exit a Graduate School Program

This is Part II in the “Should I Stay or Should I Go?” series. Part I focused on how to evaluate whether staying in graduate school is a good idea for you. In this article, I’ll discuss the logistical...
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Article

Titering Phage – Counting Something Invisible with Something Only Slightly More Visible

Titering Phage - The Plaque Assay Phage display is a molecular technique used to isolate binding or interaction partners to molecules of interest from an extensive library. Such libraries are often derived...
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Article

5 Things to Remember as a Research Intern

Make Friends as a Research Intern I used to be a supporter of the ‘lone wolf’ stance. Who needs friends or enemies? I’m completely capable of doing things on my own. Wrong and wrong again. This is...
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Article

How to perform cell synchronization in specific cell cycle phases

The cell cycle has been very well documented over the years because of its dysregulation in diseases such as cancer. Many different processes contribute to cell growth and replication, which is ultimately...
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Article

Proteomics and Good Mass Spectrometry Data

It is currently possible to analyze thousands of proteins in a single sample using mass spectrometry (MS) and a database of predicted protein sequences, referred to as ‘bottom-up’ proteomics. With this...
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Article

Four Ways to Get CRISPR Reagents Into Your Cells

Do you need to test the effects of mutating a gene in your system?  Then, CRISPR is the way to go. Your first step is to decide on good target sequences. Then, you have to get the two components of CRISPR: the...
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