Recent Articles

The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

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The Devil is in the Details: How to Setup a PCR Laboratory

Do-it-Yourself PCR

The different phases of PCR and why they are important

Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

Upcoming Webinars

Tools to make your next paper amazing

Tips and Tools for Making your Next Paper Amazing

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Date: 2nd of October, 2014
Time: 4pm London, 11am Boston, 8am San Diego
Presenter:
JODE PLANK, Head of Operations at Rubriq


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Tools To Make Your Net Paper Amazing

Choosing the Best Journal for Your Paper

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Date: 1st of October, 2014
Time: 4pm London, 11am Boston, 8am San Diego
Presenter:
BEN MUDRAK, Strategic Accounts Manager


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Tools To Make Your Net Paper Amazing

LaTeX for Biologists

Sponsored by:
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Date: 30th of September, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
JOHN HAMMERSLEY, Founder and CEO at Writelatex Limited


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Tools To Make Your Net Paper Amazing

Beginners Introduction to R Statistical Software

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Date: 29th of September, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
DR. JEREMY CHACON, University of Minnesota


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The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook

Sponsored by:
Bitesize Bio
Date: 24th of September, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
VICKI DORONINA, Post-doctoral Researcher, University of Manchester & Bitesize Bio author


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Recent Webinars

Basic methods for immunohistochemistry (Part 2)

MIKE MILLAR
Facility Manager for Immunos and Imaging at SuRF (Shared University Research Facilities)

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Basic methods for immunohistochemistry

MIKE MILLAR
Facility Manager for Immunos and Imaging at SuRF (Shared University Research Facilities)


 
 

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Simple Tips to Improve your Cloning Efficiency

If you use plasmid midi and maxi preps, your life is about to get much easier

Labfolder: Your labbook goes digital

Protein Analysis, Detection & Assay

Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

  After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way […]

Get Your Proteins! Hot Proteins Here! Radioactively Labeled Proteins!

  Radioactive protein labeling is not as common as it used to be. With the advent of modern protein labeling techniques, such as fluorescence, radioactive labeling has largely fallen out of favor. However, radioactive […]

How to Take a Cell Apart, i.e. How to Perform Subcellular Fractionation

  I do not have to tell you that eukaryotic cells are not just sacks of homogeneous protoplasm. Instead the inside of all eukaryotic cells are divided into organelles and compartments, each with unique […]

Equilibrating your way to a perfect Western blot

If you are struggling to optimise your Western blot protocol, one step to consider is the equilibration of your gel and membrane before transfer. Wondering what this step achieves and whether it’s necessary? You’re […]

You did a Co-IP…now what?

You spent the last few weeks tweaking your Co-immunoprecipitation conditions, testing different antibody/bead combinations, and sampling a panaply of solutions and FINALLY! You have your Co-immunoprecipitation (Co-IP) elution… Now what? Well, you have a […]

Microscopy & Imaging

Before you touch that microscope! The practical know-how that every microscopist must have – a Bitesize Bio eBook

In this webinar Martin Wilson will be discussing his new eBook on the basic practical know-how every microscopist must have. This guide covers the basic setup skills and knowledge you need to get started […]

ZEISS LSM 880 with Airyscan – Revolutionize Your Confocal Imaging

In this webinar you will learn About Airyscan and how it can be combined with Confocal Laser Scanning Microscopy The benefits, features and applications of Confocal LSM 880 with Airyscan – a new confocal […]

Choosing the Right Camera for Your Microscope

In this webinar you will learn the important properties of cameras how to pick the right camera to give you the best results in your specific situation Summary: Selecting the right camera for your […]

Basic methods for immunohistochemistry (Part 2)

Webinar-2014-01-31a In this webinar, I will cover basic immunohistochemistry methods and techniques, including fluorescent imaging and detection for single and multiple proteins. I will also look at ‘Sandwich Methods’ such as PAP, APPAP and […]

Analysing Microscopy Images? What You Should Know About Dynamic Range: Part 2

In the first part of this article (you can read it here), we looked at clipping and saturation in terms of microscope images, followed by a definition of Dynamic Range and an introduction to […]

Nucleic Acid Purification and Analysis

Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: “What could be the problem when one sample gives a good yield while […]

How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins […]

How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA […]

Next Generation Sequencing

Obtain NGS-Grade™ Quality Extract, Purify, & Fragment Nucleic Acids from FFPE Tissue with the Covaris Process

In this webinar you will learn: about the truXTRAC™ sample preparation system from Covaris how this system produces high-yield NGS-grade nucleic acids Summary: The controlled generation of DNA fragments is a critical sample preparation step […]

Lessons on variant hunting in next-gen data sets

Understanding the general process of how to make sense of next-gen sequencing results as they pertain to human disease. Specifically, when we sequence the whole exomes (about 20,000 genes) of patients with rare disease […]

Don’t Get Lost in RNA-seq Translation: RNA Sequencing the NGS Way

DNA sequencing (PCR, Sanger or next-generation sequencing (NGS)) is a now familiar part of any molecular biology lab. But ‘RNA-seq’, the so-called “Cinderella of genetics”, is now becoming the belle of the ball, providing […]

I Put a Smell on You: Next Generation Sequencing Sniffs Out Olfactory Signals

Chances are you spent some of your teenage years fretting about your social status. You may have even taken steps to change your status. New haircut. New clothes. These are very human behaviors: our […]

Tower of Babel: Next Generation Sequencing Provides New Insights on Chromosome Construction

Biologists have long appreciated the complexity of genome organization, but until recently lacked the tools to discern the intricacies of this puzzle. Now, thanks to some handy cross-linking, careful amplification, and (of course!) next […]

PCR & Real-time PCR

The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can […]

Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to […]

The different phases of PCR and why they are important

PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was first […]

How are crimes solved by PCR?

The scene of the crime The body of a woman is found behind an abandoned warehouse on the outskirts of town. Forensic experts carry out a technical examination of the scene and suggest strangulation […]

Flow Cytometry

Time – The Forgotten Parameter in Flow Cytometry

In this webinar you will learn: why time is a forgotten but important parameter in flow cytometry how you can study a variety of temporal changes (from seconds to days in length) using flow […]

DNA Analysis by Flow Cytometry

In this webinar you will learn different methods for analysing the cell cycle by flow cytometry answers to common questions about using flow cytometry to study the cell cycle how to properly analyse cell […]

There’s No Business Like Flow Business – Basics of Flow Cytometry

In this webinar you will learn the basics of how a flow cytometer works, including fluidics, optics and electronics. Summary: Flow Cytometry is a powerful technology that is quickly evolving.  It is important to […]

How to keep your Flow Cytometry Core Facility Happy

The helpful people who work in your flow core are an amazing resource of information for your experiments and are the people with the power to get experiments done. However, you need to be […]

Chasing the Pot of Gold at the End of the Rainbow: Choosing the Right Fluorochromes for Your Flow Cytometry

With the current proliferation of new dyes and instruments that can detect many colors simultaneously, it seems like an entire rainbow is at your disposal for your flow cytometry experiments. And we know that […]

Cell / Tissue Culture

Getting in Deep: How to Deep Clean a Tissue Culture Hood

One of the most exciting aspects of being a biologist is getting opportunities to examine how and why living organisms behave the way they do. We have technology that enables us to obtain images […]

Seven Things That Really Annoy Me About Tissue Culture

The tissue culture facility can be one of the most important places in the lab.  Many researchers spend hours in the hoods isolating primary cell lines and tissue, generating samples for western blot analysis, […]

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until […]

What is Sterile? Find Your Way around a Sterile Tissue Culture Hood

You’ve been told that maintaining a sterile environment in a tissue culture hood is vital to preventing contamination of cell cultures. But what exactly is meant by sterile? The definition of sterile is ‘completely […]

Cell Counting with a Hemocytometer: Easy as 1, 2, 3

Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Cell counting is rather straightforward and requires a counting […]

Cloning & Expression

The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook

In this webinar Vicki will be discussing her new eBook which plunges you into her years of wisdom, tips and solutions for taming your protein expression experiments.

How to identify supercoils, nicks and circles in plasmid preps

One of my favorite things to do with a student the first time they work with plasmid DNA is to have them run an agarose gel containing 2 samples:  uncut plasmid DNA, and plasmid […]

Simple Tips to Improve your Cloning Efficiency

Since the early 1970s, molecular cloning has used restriction enzymes to excise fragments from source DNA and to linearize plasmid vectors. After purifying the insert and vector, and ensuring compatible ends, both are joined […]

If you use plasmid midi and maxi preps, your life is about to get much easier

The plasmid midi and maxi preps are essential for generating high purity plasmid stocks for your precious experiments. Unfortunately, they are also tedious, time consuming, temperamental, and sometimes, utterly soul-destroying. Well, until now that […]

BioBricks: Lego for Scientists!

Lego bricks are used, mostly by children, to construct vehicles, buildings, and even working robots. Surely the idea of bringing Lego to the lab is childish, but think just for a second… since the […]

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