BLAST OFF! The Basic Local Alignment Search Tool Explained
BLAST is the most popular bioinformatics online tool. But in order to properly use it, you have to understand that is not just a search query against a database but something far more complicated, and the information you get back far more…precious!
Having brushed up on our referencing, constructing calculations and basic functions, let’s move on to some intermediate functions that could potentially save you time and hassle in the lab. First up,...
You had been happily measuring your DNA samples with your NanoDrop for a while, until… you noticed that your results are not reproducible *gulp*.
Read on – here are 10 points that will help you troubleshoot the issue.
NanoDrop for a while, until… you noticed that your results are not reproducible *gulp*.
Read on – here are 10 points that will help you troubleshoot the issue.">
Pregnancy and Post-docing: A Case Study
Deciding to start a family with your partner is one of the most exhilarating and frightening times you’ll likely ever experience, outside of welcoming the little F1 to the world that is. Your job should not...
A disruptive sequencing technology
Every new generation, a new concept is born and can completely reshape the landscape of biomedical research. Nanopore sequencing technology, although still at its infancy, is...
There are a few different ways of approaching site-directed mutagenesis. Here, I’ll give you a quick introduction to inverse PCR and why it’s useful, as well as going through a full protocol for SDM using modified primers!
How to Take a Publication-Worthy Electron Micrograph
It doesn’t matter how excited you are about the research or how intriguing the biology is, if you cannot record it – and record it well – it won’t matter. Here I will tell you how to take the perfect electron micrograph.
A Molecular Biology Roadmap For First Time Travelers
The more experienced hands in your lab know that molecular biology is rarely just a journey from A to B. As a result, I’ve constructed this short workflow as an introduction to genomic molecular techniques.
5 Ways to Improve Your Fluorescent Protein Sorting
If, like many people, you use fluorescent proteins to view your transfection efficiency or your CRISPR gene editing, you can also isolate cells with different expression levels of fluorescent proteins. Here are 5 ways to improve your sorting experiment.
The Practical Guide to Running a Perfect Homemade SDS-PAGE Gel
As biochemists, we routinely run SDS-PAGE to analyze our proteins. It can be a repetitive and tedious task to get a “presentable” gel for publication (especially when there are Western blots to follow)… but not if you know the tricks!
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