Recent Articles

BLASTing Off – How BLAST Works

More than a pun on the explosive growth of sequencing data, BLAST makes annotation and comparisons of similar sequences much easier. Created by a group at the U.S. National Center for Biotechnology Information in […]

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How to Check if Your Pipette is Accurate

Type IIS Endonucleases – When Nature Lends a Hand With DNA Cloning

Do-it-Yourself PCR

Getting started with immunohistochemistry

Recent Webinars

Basic methods for immunohistochemistry

You want to do histology? Here are the options!

Latest Products

Zymo Research’s Mix & Go! Cells: They’re Shocking… But Only to You!

Roll with it! The Award Winning optiMOS Camera from QImaging: Achieving Global Shutter Readout without Rolling Shutter Artifacts

Simple Tips to Improve your Cloning Efficiency

Protein Analysis, Detection & Assay

Getting started with immunohistochemistry

What is immunohistochemistry… Immunohistochemistry (IHC) is a favorite tool amongst clinicians to help diagnose a range of diseases by identifying abnormal cells, such as those in cancer. In a nutshell, IHC uses antibodies to detect proteins (antigens) […]

Go Fishing for Your Favorite Protein with Immunoprecipitation!

You have your favorite protein in mind and are ready to set up some exciting experiments to show what, how, when it works, modifies, and changes in your cells. Only problem? You need to […]

Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

  After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way […]

Block, Stock and Barrel – A Guide to Choosing Your Blocking Buffer

Blocking is the essential third wheel in any antibody/antigen relationship. Correct blocking buffer can perfect your antibody’s ability to bind its antigen, while bad blocking can make specific antibody binding near impossible. Don’t let […]

Get Your Proteins! Hot Proteins Here! Radioactively Labeled Proteins!

  Radioactive protein labeling is not as common as it used to be. With the advent of modern protein labeling techniques, such as fluorescence, radioactive labeling has largely fallen out of favor. However, radioactive […]

Microscopy & Imaging

Image Digitization in Microscopy: How Does That Sound?!

With the danger of becoming predictable, I will start this discussion by talking about Hi-Fi again! You must surely have heard how some sound-quality nuts (myself included) insist that vinyl sounds better than CD? […]

Roll with it! The Award Winning optiMOS Camera from QImaging: Achieving Global Shutter Readout without Rolling Shutter Artifacts

Global shutter vs rolling shutter Most of the Complementary Metal Oxide Semiconductors (CMOS) cameras on the market today offer a trade-off between their speed and the image quality. However, QImaging can now offer the […]

Come on in- the Water’s Lovely! A Look at Water Immersion Objectives

I’m guessing most people who have used a microscope will have come across oil immersion objectives- y’know- those ones which are covered in gunk and drip oil down into the sub-stage condenser?! If this […]

Analysing Microscopy Images? What You Should Know About Dynamic Range: Part 2

In the first part of this article (you can read it here), we looked at clipping and saturation in terms of microscope images, followed by a definition of Dynamic Range and an introduction to […]

Analysing Microscopy Images? What You Should Know About Dynamic Range: Part 1

Ever tried to turn the volume all the way up on a small radio or small stereo system? (Hopefully you have not tried it with earphones in!) Notice how, after some point, the sound […]

Nucleic Acid Purification and Analysis

Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: "What could be the problem when one sample gives a good yield while […]

How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins […]

How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA […]

Next Generation Sequencing

Beware The Bane of Batch Effects in NGS

 A promising study on using gene expression to develop personalized treatments for ovarian cancer A report of surprisingly high levels of differential gene expression among different ethnic groups. The announcement of previously unsuspected levels […]

Don’t Get Lost in RNA-seq Translation: RNA Sequencing the NGS Way

DNA sequencing (PCR, Sanger or next-generation sequencing (NGS)) is a now familiar part of any molecular biology lab. But ‘RNA-seq’, the so-called “Cinderella of genetics”, is now becoming the belle of the ball, providing […]

I Put a Smell on You: Next Generation Sequencing Sniffs Out Olfactory Signals

Chances are you spent some of your teenage years fretting about your social status. You may have even taken steps to change your status. New haircut. New clothes. These are very human behaviors: our […]

Tower of Babel: Next Generation Sequencing Provides New Insights on Chromosome Construction

Biologists have long appreciated the complexity of genome organization, but until recently lacked the tools to discern the intricacies of this puzzle. Now, thanks to some handy cross-linking, careful amplification, and (of course!) next […]

Ancient RNA: Does Next Generation Sequencing Offer a New Window into the Past?

The wonders of ancient DNA (‘aDNA’) have become so commonplace that they almost cease to amaze. At this point, we’ve sequenced the genomes of Neanderthals, woolly mammoths, and Pleistocene Cave Bears, and each week […]

PCR & Real-time PCR

Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR ( a project developed in order to ensure that the basic technology to […]

The different phases of PCR and why they are important

PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was first […]

Cheat on your thermocycler with Loop-mediated Isothermal amplification (LAMP)

Is it time to breakup with your thermocycler? You and your thermocycler may be joined at the hip, but is it a love-hate relationship? Does it just eat up your time and money? Perhaps […]

How are crimes solved by PCR?

The scene of the crime The body of a woman is found behind an abandoned warehouse on the outskirts of town. Forensic experts carry out a technical examination of the scene and suggest strangulation […]

Choosing the correct enzyme

A wide variety of enzymes are available for PCR and RT-PCR and the optimal choice depends on a range of factors specific to your experiment. Some of these factors will now be explored to […]

Flow Cytometry

Biosafety in Flow Cytometry – To Be or Not to Be…

Biosafety is one of those things many scientists don't take seriously. I would guess, that like politics, there are 40% who believe biosafety is 'over-emphasized' and 40% who swear by biosafety. 20% are undecided. […]

Sorting Single Cells – What Do You Need to Consider?

Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general […]

10 Useful Tips For Improving Your Sorting Experiments

After a very naïve start in flow cytometry thinking that published protocols will work without fault – needless to say, that did not work out in my favor – I realize now that following […]

An Introduction to Gating in Flow Cytometry

What is one of the first things you do when you sit down at the flow cytometer and start looking at your cells? You start drawing polygons and setting gates. To the neophyte the […]

How to keep your Flow Cytometry Core Facility Happy

The helpful people who work in your flow core are an amazing resource of information for your experiments and are the people with the power to get experiments done. However, you need to be […]

Cell / Tissue Culture

Getting in Deep: How to Deep Clean a Tissue Culture Hood

One of the most exciting aspects of being a biologist is getting opportunities to examine how and why living organisms behave the way they do. We have technology that enables us to obtain images […]

Seven Things That Really Annoy Me About Tissue Culture

The tissue culture facility can be one of the most important places in the lab.  Many researchers spend hours in the hoods isolating primary cell lines and tissue, generating samples for western blot analysis, […]

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until […]

What is Sterile? Find Your Way around a Sterile Tissue Culture Hood

You’ve been told that maintaining a sterile environment in a tissue culture hood is vital to preventing contamination of cell cultures. But what exactly is meant by sterile? The definition of sterile is ‘completely […]

Cell Counting with a Hemocytometer: Easy as 1, 2, 3

Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Cell counting is rather straightforward and requires a counting […]

Cloning & Expression

Type IIS Endonucleases – When Nature Lends a Hand With DNA Cloning

Good news lab workers! Always hated the tedious work of designing a cloning strategy? Or maybe always dreamed of pooling all the reactions in one tube, just to save time? Thanks to Mother Nature, […]

Choosing a Competent E.coli Strain

Of all the of competent E. coli cell strains available, which one should you choose? The choice of strain to use in a given experiment is determined in large part by the nature of […]

DIY Electrocompetent E. coli

If you buy competent E.coli regularly, you'll know that they are pretty expensive. So the cost of screwing up a cloning or transformation experiment is pretty high in terms of money, as well as […]

E.coli Electroporation vs Chemical Transformation

This is the first in a three part series on the transformation of E.coli. By the end of this you should be an expert on E.coli transformation and on which strains to choose for […]

Zymo Research’s Mix & Go! Cells: They’re Shocking… But Only to You!

I have a secret to tell you. Are you ready? You don’t have to heat shock your bacterial cells anymore to transform them.  Don’t bother putting them in a water bath for exactly 2 minutes, […]

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