Important Considerations for a Career in Biotech

When I was in graduate school, it seemed that almost no one aspired to work in industry or be part of a company. But times are changing. Now, when I go to conferences [read more]

Comments

on Ligation Independent Cloning Primer Design
Dear All, I have been trying to ligate a Sac1 Sal1 adaptor i designed into a vector but have not been successful. The [read more]
on Pimp Your Plasmid Growth Medium
Hey Sean!! It's Catherine from the Margolick lab!! Thought I recognized the dreads... Just sent a "friend request". [read more]
on The Art of PCR Primer Design
Does anyone have any experience with nucleotide/nucleoside analogues. I am working with HIV and trying to design some [read more]
on Pimp Your Plasmid Growth Medium
hi there, anyone try this media for recombinant protein expression? if so, how did it fare compared with lb? S
on Pimp your Microcentrifuge
Good idea on marking the rotor for 3 tubes Jode. One of those tiny (perhaps tragic) pleasures is when you drop the 3 [read more]

Questions

Answers

on co-extract DNA and RNA
I would suggest contacting a Qiagen representative directly. They will know all the specs of the kits. They will [read more]
on DNA extraction… white precipitate: have I lost my desired DNA??
A white/translucent pellet after isopropanol addition is normal. Is the pellet before the isopropanol addition at the [read more]
on Why does the DNA precipitate pellet get washed off the wall easily even after centrifugation?
Could it have something to do with the plastic tubes you are using? Maybe the plastic is glossy and meant to keep [read more]

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