Recent Articles

A Beginner’s Guide to Next Generation Sequencing (NGS) Technology

The first whole DNA genome to be sequenced was the virus phage ΦX174, in 1977, by Fred Sanger. In that same year, Sanger developed the future backbone of the “genome era”: DNA sequencing technology. […]

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Antibodies 101: Every Body Needs an Anti-Body!

The 9 Online Calculators You Need to Know About

Protocols: Where Do You Get Yours?

Analyzing Apoptosis – A Review of Analytical Techniques

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Tips and Tools for Making your Next Paper Amazing

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Time: 4pm London, 11am Boston, 8am San Diego
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If English is My Native Language, Why Would I Need Editing

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Date: 3rd of October, 2014
Time: 4pm London, 11am Boston, 8am San Diego
Presenter:
KURT SPURLOCK, at American Journal Experts/Research Square


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Alternative careers for scientists

Using Both the Right and Left Parts of Your Brain to Get the Perfect Job

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Date: 7th of October, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
WENDY OCHOA, PHD, Marketing Communication Manager, MO BIO laboratories Founder, Owner and Freelance Web/Graphic designer AyAyAy Design Studio


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Tools To Make Your Next Paper Amazing

Choosing the Best Journal for Your Paper

BEN MUDRAK, PHD
Strategic Accounts Manager at Research Square

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Lay out your manuscripts, theses and reports like a pro with LaTeX for Biologists

JOHN HAMMERSLEY, PHD
Founder and CEO at Writelatex Limited

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Latest Products

To INFINITY and Beyond! A Detailed Look at Lumenera’s INFINITY3-3UR Microscopy Camera

CCD vs sCMOS Cameras for Microscopy. The optiMOS Camera from QImaging is Fast, Sensitive and Budget Friendly!

To INFINITY and Beyond! The INFINITY series of fluorescence microscopy cameras from Lumenera®

Protein Analysis, Detection & Assay

Antibodies 101: Every Body Needs an Anti-Body!

Antibodies are one of the most important tools in molecular biology. Regardless of what species, cell type, or organ system you are studying, it is almost inevitable that you will use an antibody-based assay […]

Know Your Western Blot Jargon! A Quick Review.

It has been said that "Incomprehensible jargon is the hallmark of a profession" (Kingman Brewster, Jr) and while I cannot speak for other professions, as a biologist I am inclined to believe it. So […]

Get your stripping stripes! Find out how to strip and re-blot your Western

Westerns can be tricky and time-consuming, so make the most of your precious membranes and their proteins. Learn how to properly strip off your antibodies and re-probe with another primary antibody. Why you should […]

Getting started with Immunohistochemistry

What is immunohistochemistry… Immunohistochemistry (IHC) is a favorite tool amongst clinicians to help diagnose a range of diseases by identifying abnormal cells, such as those in cancer. In a nutshell, IHC uses antibodies to detect proteins (antigens) […]

Go Fishing for Your Favorite Protein with Immunoprecipitation!

You have your favorite protein in mind and are ready to set up some exciting experiments to show what, how, when it works, modifies, and changes in your cells. Only problem? You need to […]

Microscopy & Imaging

Looking good! A Guide to Adjusting and Maintaining Microscope Eyepieces

The magnification and viewing of samples using a microscope relies on both the objectives and the eyepieces working harmoniously together. If you buy a ready-to-use microscope, then the objectives and the eyepieces which are […]

Stereo (Dissecting) Microscopes 101

  Think a stereo microscope might be helpful? But you don’t know how to use one? Lucky for you I wrote this primer on stereo microscopes to help you figure out when and how […]

Taken for Granted: Position and Setup of Your Microscope

Although I say ‘taken for granted’, over the years of working in and managing microscope facilities, it quickly became clear that the position of the microscope, user position and set-up were aspects which were […]

The Who’s Who of Super Resolution Microscopy, Part 1

The 'diffraction limit' of a microscope is the minimum distance between two fluorophores where they can be still be discriminated as two separate objects. This diffraction limit has long constrained attempts by biologists to […]

ZEISS LSM 880 with Airyscan – Revolutionize Your Confocal Imaging

Webinar-2014-09-16-ZEISS LSM 880 with Airyscan Revolutionize Your Confocal Imaging In this webinar you will learn About Airyscan and how it can be combined with Confocal Laser Scanning Microscopy The benefits, features and applications of […]

Nucleic Acid Purification and Analysis

Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: "What could be the problem when one sample gives a good yield while […]

How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins […]

How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA […]

Next Generation Sequencing

A Beginner’s Guide to Next Generation Sequencing (NGS) Technology

The first whole DNA genome to be sequenced was the virus phage ΦX174, in 1977, by Fred Sanger. In that same year, Sanger developed the future backbone of the “genome era”: DNA sequencing technology. […]

Beware The Bane of Batch Effects

A promising study on using gene expression to develop personalized treatments for ovarian cancer. A report of surprisingly high levels of differential gene expression among different ethnic groups. The announcement of previously unsuspected levels […]

Sequencing the microbiome: re-discovering our invisible allies

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case […]

Obtain NGS-Grade™ Quality Extract, Purify, & Fragment Nucleic Acids from FFPE Tissue with the Covaris Process

In this webinar you will learn: about the truXTRAC™ sample preparation system from Covaris how this system produces high-yield NGS-grade nucleic acids Summary: The controlled generation of DNA fragments is a critical sample preparation step […]

Lessons on variant hunting in next-gen data sets

Understanding the general process of how to make sense of next-gen sequencing results as they pertain to human disease. Specifically, when we sequence the whole exomes (about 20,000 genes) of patients with rare disease […]

PCR & Real-time PCR

The History of PCR

As with some of the greatest discoveries in science, from penicillin to microwave ovens and play-doh, PCR was discovered serendipitously. Thanks to the work of many scientists, including Watson and Crick, Kornberg, Khorana, Klenow, […]

Mischief in the Mastermix: Bothersome Bubbles

It happens to the best of us. You’re minding your own business and suddenly out of nowhere your mastermix is a bubble bath and your primers are enjoying a froth party. Let’s talk about […]

MIQE Guidelines for Digital PCR

MIQE what's that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for minimum information […]

The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can […]

Flow Cytometry

One For You, One For Me… How a Cell Sorter Works

Cell sorters do not operate by magic, even it looks that way. It’s about the application of physics, electronics, fast computers and formation of droplets. Whether you bring your cells to a flow core […]

Crap in, crap out: Flushing Out The Problems in Your Flow Cytometry Data

“What Have You Done To My Cells??!!!” This cry of pain from researchers, frequently aimed at core facility operators, is heard after receiving incomprehensible data for an invaluable tube of cells. Equally baffling to […]

All for one and one for all – Fluorescence Minus One Controls

Ever done a multicolour flow cytometry experiment, run all your controls, done your compensation and then started to analyse your data and realised that you can’t work out where to put your gates? To […]

Time – The Forgotten Parameter in Flow Cytometry

In this webinar you will learn: why time is a forgotten but important parameter in flow cytometry how you can study a variety of temporal changes (from seconds to days in length) using flow […]

DNA Analysis by Flow Cytometry

In this webinar you will learn different methods for analysing the cell cycle by flow cytometry answers to common questions about using flow cytometry to study the cell cycle how to properly analyse cell […]

Cell / Tissue Culture

Getting in Deep: How to Deep Clean a Tissue Culture Hood

One of the most exciting aspects of being a biologist is getting opportunities to examine how and why living organisms behave the way they do. We have technology that enables us to obtain images […]

Seven Things That Really Annoy Me About Tissue Culture

The tissue culture facility can be one of the most important places in the lab.  Many researchers spend hours in the hoods isolating primary cell lines and tissue, generating samples for western blot analysis, […]

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until […]

What is Sterile? Find Your Way around a Sterile Tissue Culture Hood

You’ve been told that maintaining a sterile environment in a tissue culture hood is vital to preventing contamination of cell cultures. But what exactly is meant by sterile? The definition of sterile is ‘completely […]

Cell Counting with a Hemocytometer: Easy as 1, 2, 3

Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Cell counting is rather straightforward and requires a counting […]

Cloning & Expression

A Sneak Peak at ‘The Bitesize Bio Guide to Protein Expression’ – a Bitesize Bio eBook

If I piqued your interest in the first post about my new e-Book ‘The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook’ check out this excerpt from the book explaining what […]

The Lac Operon Explained

An operon is a functioning unit of genomic DNA that contains a group of genes controlled by a single promoter. Put simply, these genes share information needed to create the tools for a particular […]

Have I Got Good Protein Expression News for You

While the art of reading hand poured sequencing gels is forgotten as the next generation sequencers spit out genome after genome, protein expression still often requires sweat, tears and a lot of luck. I […]

Overlap Extension PCR Cloning

If efficient cloning is what you are after, you must give Overlap Extension PCR Cloning a go! This restriction enzyme and T4 DNA ligase-free technique is faster, more reliable and easier to troubleshoot than […]

The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook

TheBitesizeBioGuidetoProteinExpression In this webinar Vicki discusses her new eBook which plunges you into her years of wisdom, tips and solutions for taming your protein expression experiments. Get the eBook Click here or on the image below […]

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