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Ensure Reproducibility: Control for Lot-to-Lot Variation of Antibodies

When starting a long-term experiment, you need to take a lot of things into consideration (availability of cells, reagents, planning time points), but do you ever think about your antibodies? If you buy an...
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SPUD’s Your Bud When it Comes to Sensitive qPCR

There's piloting a brand new technique for the first time. Then, there’s jumping through hoops trying to get an established lab technique to work. The former, in contrast to the latter, is expected to be...
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Immunohistochemistry: Getting The Stain You Want

Immunohistochemistry (IHC) is a vital tool, not just at your wet bench but also in clinical labs the world over. IHC is used extensively in hospitals and veterinary practices in grading and studying cancers,...
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How to Make the Most of Your Visit to a Collaborating Lab

One of the many perks that we often experience as graduate students is the chance to work with a collaborating lab on a research project. A successful collaboration results in: Gaining a collaborator’s...
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The World of Microbes (II): The Gut Microbiome in Health and Disease

If you google “What’s hot in medicine,” you will see the word “gut microbiome” popping up in every corner of the webpage. Microbes keep your body functioning in balance - from digesting complex...
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Introducing CyTOF: Cytometry of the Masses

Flow cytometry remains unparalleled as a single-cell analysis technology.  The ability to analyze 14 or more fluorescent parameters on a million cells or more allows for detailed understanding of complex...
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Safety Comes First: Common Laboratory Signs

Our laboratory is full of signs isn’t it? Be it the chemical bottles or the signs near the basin, they are everywhere.  But that’s a good thing and is important for our safety. These signs tell you about...
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Commercialize Reagents for Fun and Profit

How long has it been since you checked your lab freezer? Remember that plasmid you designed? How about that cell line you developed that now sits idly in the vapors of a liquid Nitrogen tank? And the novel...
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How SDS-PAGE works

What does a stacking gel do? This and other questions about SDS-PAGE answered!
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What Comes Next After Completing Your Undergraduate Degree?

As your undergraduate years wind down to completion, you will ultimately ask yourself, what next? Fortunately, as a life sciences student, there are many paths you could take and the hardest part may be...
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