Recent Articles

SuperSize It! Scaling Up Your Experiments

Life in the lab is easy-peasy when you are only prepping a handful of tubes. But what if you need to scale up to 10’s or even 100’s of samples? Scaling up your experiments […]

view article »

Know Your Western Blot Jargon! A Quick Review.

Take Control of Your World – Five Controls for Flow Cytometry

Help: My Gal Promoter Doesn’t Work

Dpn I The Strange

Recent Webinars

Basic methods for immunohistochemistry

You want to do histology? Here are the options!

Latest Products

CCD vs sCMOS Cameras for Microscopy. The optiMOS Camera from QImaging is Fast, Sensitive and Budget Friendly!

To INFINITY and Beyond! The INFINITY series of fluorescence microscopy cameras from Lumenera®

Zymo Research’s Mix & Go! Cells: They’re Shocking… But Only to You!

Protein Analysis, Detection & Assay

Know Your Western Blot Jargon! A Quick Review.

It has been said that "Incomprehensible jargon is the hallmark of a profession" (Kingman Brewster, Jr) and while I cannot speak for other professions, as a biologist I am inclined to believe it. So […]

Get your stripping stripes! Find out how to strip and re-blot your Western

Westerns can be tricky and time-consuming, so make the most of your precious membranes and their proteins. Learn how to properly strip off your antibodies and re-probe with another primary antibody. Why you should […]

Getting started with immunohistochemistry

What is immunohistochemistry… Immunohistochemistry (IHC) is a favorite tool amongst clinicians to help diagnose a range of diseases by identifying abnormal cells, such as those in cancer. In a nutshell, IHC uses antibodies to detect proteins (antigens) […]

Go Fishing for Your Favorite Protein with Immunoprecipitation!

You have your favorite protein in mind and are ready to set up some exciting experiments to show what, how, when it works, modifies, and changes in your cells. Only problem? You need to […]

Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

  After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way […]

Microscopy & Imaging

I Can See (See Dee)! CCD and CMOS Cameras for Micoscopy

Two different sensors are generally used in cameras for microscopy: Charge Coupled Devices (CCD) or Complementary Metal Oxide Semiconductors (CMOS or sCMOS). Although there are a number of similarities between the two sensors, differences […]

CCD vs sCMOS Cameras for Microscopy. The optiMOS Camera from QImaging is Fast, Sensitive and Budget Friendly!

CCD cameras – the previous gold standard Since the inception of digital microscopy, scientific grade CCD cameras have been the gold standard for imaging due to their sensitivity, linear response to light and low […]

To INFINITY and Beyond! The INFINITY series of fluorescence microscopy cameras from Lumenera®

You did it! You got your perfect microscopy specimen – you fixed it, you mounted it, you labeled it with your favorite fluorophore – now there is only one step left: To image it. […]

What Matters When Choosing Your Histology Mounting Media

After successfully collecting and processing your sample, there is only one step between you and your finished slides – mounting. Correct mounting is important for good imaging results and proper sample preservation. Read below […]

Image Digitization in Microscopy: How Does That Sound?!

With the danger of becoming predictable, I will start this discussion by talking about Hi-Fi again! You must surely have heard how some sound-quality nuts (myself included) insist that vinyl sounds better than CD? […]

Nucleic Acid Purification and Analysis

Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: "What could be the problem when one sample gives a good yield while […]

How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins […]

How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA […]

Next Generation Sequencing

Next Gen Sequencing or Microarray: How to Make the Choice

You’re about to start that big project you’ve been dreaming of for years. You’ve identified a potential miracle compound and want to figure out how it affects gene expression. But how are you going […]

Beware The Bane of Batch Effects in NGS

 A promising study on using gene expression to develop personalized treatments for ovarian cancer A report of surprisingly high levels of differential gene expression among different ethnic groups. The announcement of previously unsuspected levels […]

Don’t Get Lost in RNA-seq Translation: RNA Sequencing the NGS Way

DNA sequencing (PCR, Sanger or next-generation sequencing (NGS)) is a now familiar part of any molecular biology lab. But ‘RNA-seq’, the so-called “Cinderella of genetics”, is now becoming the belle of the ball, providing […]

I Put a Smell on You: Next Generation Sequencing Sniffs Out Olfactory Signals

Chances are you spent some of your teenage years fretting about your social status. You may have even taken steps to change your status. New haircut. New clothes. These are very human behaviors: our […]

Tower of Babel: Next Generation Sequencing Provides New Insights on Chromosome Construction

Biologists have long appreciated the complexity of genome organization, but until recently lacked the tools to discern the intricacies of this puzzle. Now, thanks to some handy cross-linking, careful amplification, and (of course!) next […]

PCR & Real-time PCR

The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can […]

Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to […]

The different phases of PCR and why they are important

PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was first […]

Cheat on your thermocycler with Loop-mediated Isothermal amplification (LAMP)

Is it time to breakup with your thermocycler? You and your thermocycler may be joined at the hip, but is it a love-hate relationship? Does it just eat up your time and money? Perhaps […]

Flow Cytometry

Take Control of Your World – Five Controls for Flow Cytometry

No, this is not a call for Geeks to take over the world – just a tiny part of it – the part that ensures success in all experiments or at least a good […]

Intracellular Cytokine Staining: Letting It All Build Up Inside

Cytokines, those small proteins that modulate immune cell responses, once translated are normally secreted rapidly out of the cell. So, previously we could only check the levels of cytokines secreted in the supernatant, but we wouldn't know which […]

Cell Proliferation Round 2 And Beyond: The Dye Dilution Method

Like the legendary fight between boxers Bowen and Burke in 1893, the cell cycle in some cells goes on and on..round 1, round 2, round 3, round 4…before the final bell is rung. Nucleotide […]

Biosafety in Flow Cytometry – To Be or Not to Be…

Biosafety is one of those things many scientists don't take seriously. I would guess, that like politics, there are 40% who believe biosafety is 'over-emphasized' and 40% who swear by biosafety. 20% are undecided. […]

Sorting Single Cells – What Do You Need to Consider?

Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general […]

Cell / Tissue Culture

Getting in Deep: How to Deep Clean a Tissue Culture Hood

One of the most exciting aspects of being a biologist is getting opportunities to examine how and why living organisms behave the way they do. We have technology that enables us to obtain images […]

Seven Things That Really Annoy Me About Tissue Culture

The tissue culture facility can be one of the most important places in the lab.  Many researchers spend hours in the hoods isolating primary cell lines and tissue, generating samples for western blot analysis, […]

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until […]

What is Sterile? Find Your Way around a Sterile Tissue Culture Hood

You’ve been told that maintaining a sterile environment in a tissue culture hood is vital to preventing contamination of cell cultures. But what exactly is meant by sterile? The definition of sterile is ‘completely […]

Cell Counting with a Hemocytometer: Easy as 1, 2, 3

Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Cell counting is rather straightforward and requires a counting […]

Cloning & Expression

Dpn I The Strange

If you ever used a site-directed mutagenesis kit or ligation-independent cloning, then you also used restriction enzyme Dpn I. But what does it do and more interestingly, why? Restriction enzymes and methylases: the yin […]

Type IIS Endonucleases – When Nature Lends a Hand With DNA Cloning

Good news lab workers! Always hated the tedious work of designing a cloning strategy? Or maybe always dreamed of pooling all the reactions in one tube, just to save time? Thanks to Mother Nature, […]

Choosing a Competent E.coli Strain

Of all the of competent E. coli cell strains available, which one should you choose? The choice of strain to use in a given experiment is determined in large part by the nature of […]

DIY Electrocompetent E. coli

If you buy competent E.coli regularly, you'll know that they are pretty expensive. So the cost of screwing up a cloning or transformation experiment is pretty high in terms of money, as well as […]

E.coli Electroporation vs Chemical Transformation

This is the first in a three part series on the transformation of E.coli. By the end of this you should be an expert on E.coli transformation and on which strains to choose for […]

Share your tips, advice & wisdom on Bitesize Bio

We gather the best tips, advice and wisdom from you guys at the bench and publish them to help each other improve in the lab.

What could you add to this collection? Click here to explore how you could contribute to Bitesize Bio.