Recent Articles

One For You, One For Me… How a Cell Sorter Works

Cell sorters do not operate by magic, even it looks that way. It’s about the application of physics, electronics, fast computers and formation of droplets. Whether you bring your cells to a flow core […]

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The History of PCR

How To Calculate The Number Of Molecules In Any Piece of DNA

What Can NMR Do For You – Part Three

Beware The Bane of Batch Effects

Upcoming Webinars

Writing with Bitesize Bio

Dive into Science writing, presenting & editing at Bitesize Bio

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Date: 22nd of September, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
NICK OSWALD, Founder of Bitesize Bio


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Writing with Bitesize Bio

Before you touch that microscope! The practical know-how that every microscopist must have – a Bitesize Bio eBook

Sponsored by:
Date: 23rd of September, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
MARTIN WILSON, Founder @ Hatch, Burn, Carve & Bitesize Bio author


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The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook

Sponsored by:
Date: 24th of September, 2014
Time: 5pm London, 12pm Boston, 9am San Diego
Presenter:
VICKI DORONINA, Post-doctoral Researcher, University of Manchester & Bitesize Bio author


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Recent Webinars

Enhancing your microscopy toolbox

Choosing the Right Camera for Your Microscope

DR. DOUGLAS RICHARDSON
Director of Imaging at the Harvard Center for Biological Imaging

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Enhancing your microscopy toolbox

ZEISS LSM 880 with Airyscan – Revolutionize Your Confocal Imaging

CHRIS POWER
3D Imaging Specialist, ZEISS

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Latest Products

To INFINITY and Beyond! A Detailed Look at Lumenera’s INFINITY3-3UR Microscopy Camera

To INFINITY and Beyond! The INFINITY series of fluorescence microscopy cameras from Lumenera®

Simple Tips to Improve your Cloning Efficiency

Protein Analysis, Detection & Assay

Getting started with Immunohistochemistry

What is immunohistochemistry… Immunohistochemistry (IHC) is the process of using antibodies to detect proteins (antigens) in cells within a tissue section (for example the liver, pancreas or the heart). …and why would I want […]

Get your stripping stripes! Find out how to strip and re-blot your Western

Westerns can be tricky and time-consuming, so make the most of your precious membranes and their proteins. Learn how to properly strip off your antibodies and re-probe with another primary antibody. Why you should […]

Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

  After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way […]

Get Your Proteins! Hot Proteins Here! Radioactively Labeled Proteins!

  Radioactive protein labeling is not as common as it used to be. With the advent of modern protein labeling techniques, such as fluorescence, radioactive labeling has largely fallen out of favor. However, radioactive […]

How to Take a Cell Apart, i.e. How to Perform Subcellular Fractionation

  I do not have to tell you that eukaryotic cells are not just sacks of homogeneous protoplasm. Instead the inside of all eukaryotic cells are divided into organelles and compartments, each with unique […]

Microscopy & Imaging

The Who’s Who of Super Resolution Microscopy, Part 1

The 'diffraction limit' of a microscope is the minimum distance between two fluorophores where they can be still be discriminated as two separate objects. This diffraction limit has long constrained attempts by biologists to […]

ZEISS LSM 880 with Airyscan – Revolutionize Your Confocal Imaging

Webinar-2014-09-16-ZEISS LSM 880 with Airyscan Revolutionize Your Confocal Imaging In this webinar you will learn About Airyscan and how it can be combined with Confocal Laser Scanning Microscopy The benefits, features and applications of […]

To INFINITY and Beyond! A Detailed Look at Lumenera’s INFINITY3-3UR Microscopy Camera

Are you trying to capture a fast imaging event in low light conditions? Well then you need a high-speed, highly sensitive camera like Lumenera’s INFINITY3-3UR microscopy camera. Previously in “To INFINITY and Beyond!” I […]

Before you touch that microscope! The practical know-how that every microscopist must have – a Bitesize Bio eBook

In this webinar Martin Wilson will be discussing his new eBook on the basic practical know-how every microscopist must have. This guide covers the basic setup skills and knowledge you need to get started […]

Choosing the Right Camera for Your Microscope

Choosing the right camera for your microscope In this webinar you will Get to grips with the essential camera characteristics like pixel count,  spectral response, noise, and read-out time Understand when color or monochrome, CCD or […]

Nucleic Acid Purification and Analysis

Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: "What could be the problem when one sample gives a good yield while […]

How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins […]

How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA […]

Next Generation Sequencing

Beware The Bane of Batch Effects

A promising study on using gene expression to develop personalized treatments for ovarian cancer. A report of surprisingly high levels of differential gene expression among different ethnic groups. The announcement of previously unsuspected levels […]

Sequencing the microbiome: re-discovering our invisible allies

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case […]

Obtain NGS-Grade™ Quality Extract, Purify, & Fragment Nucleic Acids from FFPE Tissue with the Covaris Process

In this webinar you will learn: about the truXTRAC™ sample preparation system from Covaris how this system produces high-yield NGS-grade nucleic acids Summary: The controlled generation of DNA fragments is a critical sample preparation step […]

Lessons on variant hunting in next-gen data sets

Understanding the general process of how to make sense of next-gen sequencing results as they pertain to human disease. Specifically, when we sequence the whole exomes (about 20,000 genes) of patients with rare disease […]

Next Gen Sequencing or Microarray: How to Make the Choice

You’re about to start that big project you’ve been dreaming of for years. You’ve identified a potential miracle compound and want to figure out how it affects gene expression. But how are you going […]

PCR & Real-time PCR

The History of PCR

As with some of the greatest discoveries in science, from penicillin to microwave ovens and play-doh, PCR was discovered serendipitously. Thanks to the work of many scientists, including Watson and Crick, Kornberg, Khorana, Klenow, […]

MIQE Guidelines for Digital PCR

MIQE what's that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for minimum information […]

The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can […]

Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to […]

Flow Cytometry

One For You, One For Me… How a Cell Sorter Works

Cell sorters do not operate by magic, even it looks that way. It’s about the application of physics, electronics, fast computers and formation of droplets. Whether you bring your cells to a flow core […]

Crap in, crap out: Flushing Out The Problems in Your Flow Cytometry Data

“What Have You Done To My Cells??!!!” This cry of pain from researchers, frequently aimed at core facility operators, is heard after receiving incomprehensible data for an invaluable tube of cells. Equally baffling to […]

All for one and one for all – Fluorescence Minus One Controls

Ever done a multicolour flow cytometry experiment, run all your controls, done your compensation and then started to analyse your data and realised that you can’t work out where to put your gates? To […]

Time – The Forgotten Parameter in Flow Cytometry

In this webinar you will learn: why time is a forgotten but important parameter in flow cytometry how you can study a variety of temporal changes (from seconds to days in length) using flow […]

DNA Analysis by Flow Cytometry

In this webinar you will learn different methods for analysing the cell cycle by flow cytometry answers to common questions about using flow cytometry to study the cell cycle how to properly analyse cell […]

Cell / Tissue Culture

Getting in Deep: How to Deep Clean a Tissue Culture Hood

One of the most exciting aspects of being a biologist is getting opportunities to examine how and why living organisms behave the way they do. We have technology that enables us to obtain images […]

Seven Things That Really Annoy Me About Tissue Culture

The tissue culture facility can be one of the most important places in the lab.  Many researchers spend hours in the hoods isolating primary cell lines and tissue, generating samples for western blot analysis, […]

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until […]

What is Sterile? Find Your Way around a Sterile Tissue Culture Hood

You’ve been told that maintaining a sterile environment in a tissue culture hood is vital to preventing contamination of cell cultures. But what exactly is meant by sterile? The definition of sterile is ‘completely […]

Cell Counting with a Hemocytometer: Easy as 1, 2, 3

Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Cell counting is rather straightforward and requires a counting […]

Cloning & Expression

Overlap Extension PCR Cloning

If efficient cloning is what you are after, you must give Overlap Extension PCR Cloning a go! This restriction enzyme and T4 DNA ligase-free technique is faster, more reliable and easier to troubleshoot than […]

The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook

In this webinar Vicki will be discussing her new eBook which plunges you into her years of wisdom, tips and solutions for taming your protein expression experiments.

Dpn I The Strange

If you ever used a site-directed mutagenesis kit or ligation-independent cloning, then you also used restriction enzyme Dpn I. But what does it do and more interestingly, why? Restriction enzymes and methylases: the yin […]

How to identify supercoils, nicks and circles in plasmid preps

One of my favorite things to do with a student the first time they work with plasmid DNA is to have them run an agarose gel containing 2 samples:  uncut plasmid DNA, and plasmid […]

Simple Tips to Improve your Cloning Efficiency

Since the early 1970s, molecular cloning has used restriction enzymes to excise fragments from source DNA and to linearize plasmid vectors. After purifying the insert and vector, and ensuring compatible ends, both are joined […]

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