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Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
Coffee is powerful stuff, but did you ever think of using it for….
CIP has been around for a while, but there is a better alternative.
If you regularly grow up the same bacterial culture, whether it’s the strain that expresses your favorite protein, the culture you make your competent cells from, or just your regular control strain, it can be a bit of a pain growing it up from scratch each time. Before you even get to grow your actual…
The humble plasmid. We now know it so well, but as little as 60 years ago the field of extra-chromosomal heredity was decidedly murky. Not only was it the subject of great debate, conflict and friction within the scientific community, it was even used as a politico-religious tool during the Cold War! The origin of…
There’s something in the water, and it would love to go after your experiments. Straight out of the tap, water contains microorganisms, endotoxins, DNase and RNase, salts and other impurities that could gobble up your experiment in one bite. Of course we avoid this drama completely by using purified water from which these nasties have…
How often do you make errors in the lab that ruin a good experiment? Rather than flaws in experimental design, I mean errors like forgetting to add a reagent, pipetting the wrong amount, or following a protocol step wrongly. Especially early on in your career, errors like this can be a real drain on your…
TAE or TBE, which is best? Well, of course, it depends on what you want to do. Here are the pros and cons of both: But you might be better of using neither of these buffers. Despite the fact that they have been firmly established as the most popular buffers for DNA electrophoresis since their…
My PhD was a soul-less affair. It was also rock-less, jazz-less and pop-less. And all because my supervisor was of the opinion that music in the lab was a distraction that reduced concentration and our ability to do the job. “Rubbish!”, I thought, “Nothing helps you through a mindless task like splitting cells, pipetting or…
You youngsters don’t know how easy you’ve got it. Kits, outsourcing and improved practices are making research easier and easier. At least in theory (who are we kidding?). In the old days things were much tougher, and many wiley old scientists bear the scars, mental and physical, of carrying out techniques that were mind numbing,…
So you finally got your PhD (or your masters or batchelor’s) and you are making the big switch to a small biotech company. You will probably have been hired for the specific skill set that you have built during your training, but now you have to learn to apply those skills to solve real world,…
The following question was emailed to Bitesize Bio by Beheroze Sattha and I gladly took up the challenge, and I immediately knew the answer. Or so I thought. After delving extensively into Pubmed, Genes V (I know, I need a new version) and Molecular Cloning I have come up with an answer, but it is…
Criticism is not just valuable, it is essential for a person’s development as a scientist, or anything else for that matter. Well that’s not entirely true. Not all criticism is valuable, it has to be the right kind of criticism. It has to be constructive and better still, well delivered in order to inspire the…
Really great presentation skills. Some people in science seem to have them, and some don’t. I am one of the don’ts. Sure, I can get up in front of people and talk when needed, but it won’t be a polished performance by any means. I can get my message across but my delivery is not…
Keeping safe in the lab really only requires one thing: common sense. But if you look at what people are doing in the lab, you might think that common sense isn’t so common after all. What are the most stupid things you have seen people do in the lab to put the safety of themselves…
No matter how many times you look at it, it’s not going to change. You are planning your next cloning experiment, but there’s a problem. The only restriction enzyme that cuts in a suitable position on your plasmid vector also, as luck would have it, cuts in another position elsewhere in the vector so you…
Going to conferences normally involves a significant investment of time and money. So it’s important to get as much as you can out of them. One of the most valuable things you can get from a conference is contacts. These can build into a network of people that will be valuable to you throughout your…
RPM and RCF are two units that can be used to describe the speed of a centrifuge. Although they may look similar, they are oh-so-different and confusing them has resulted a disastrous end to many an experiment. So let’s set it out in black and white to make sure you don’t succumb to the same…
After ligation, the method you use for desalting your sample prior to electroporation is critical, especially if your ligation is inefficient, according to a study by Schlaak et al [1]. Under standard electroporation conditions, the electric field of 12-18 kV/cm generated in a 0.1mm-gap electroporation cuvette means that the conductivity of the sample must be…
If you search the literature using a comprehensive search engine like Google Scholar, you will get several types of articles listed. Most of them are peer reviewed journal articles and many are patents. But beware of an important distinction between the two: Although patents can contain useful information, they are not authoritative because they are not…
Undergrad courses teach you to learn in a specific way. You have to cram in as much information into your brain as possible, hold it in there, then regurgitate as much of it as possible on exam day. Of course, actually understanding what you are talking about, and working from basic principles, helps but the…
There is nothing more frustrating than getting back rubbish data from a DNA sequencing run, especially when you are waiting for an important result. For example, confirmation of that clone you have been trying to get for the past three months! A lot of the time, the quality of sequencing data is within your control….
No scientist is an island, not even a great scientist like you. A good network of professional contacts is as essential to your career as hard work at the bench. This is because your network can open many doors that no amount of good bench work could. Access to unpublished information, collaborations and job opportunities…
If you use a spec to measure cell density, you may be making a very common mistake and taking inaccurate measurements as a result. Specs are often used for measuring the density of suspension cultures, but the mistake that many people make is to record the OD given by the spec as an absolute value….
Everyone is worried about getting results, aren’t they? Results are what you need for success in science – they are essential for bringing the funding in. But focusing on results per se is not a good way to work because, as a scientist, you can’t “get” results. You can’t “make” them happen. Essentially in every…
You are not alone. Everyone makes a hash of their protein gel sometimes but this resource can help you work out what went wrong, and feel better for seeing gels even worse than yours.
Your DNA samples are precious so take care of them! Here are 5 ways that DNA can be damaged, so now you now what to avoid in the future.
Ever heard of polymerising agarose gels? I haven’t. If you think you have, read this.
DEPC. IF you work with RNA, you’ll know this stuff. It’s vital for ridding solutions of RNases that would otherwise destroy your work. But just how well do you know it?
This is the story of how one of the most famous and quirky naming conventions in biology came into being.
Calculating your fudge ratio can help you get your work done on time and get home before dark. Here’s how.
Biology researchers have a bad habit of doing accurate assays using semi-accurate tools. Here are some suggestions on how to sharpen up the accuracy of your assays.
You probably use and rely them more than any other tool in the lab, but just how accurate are your pipettes?
As far as your lab work is concerned, your lab book is the source of all knowledge. Making it easy to find things in there will help. Here’s how to do it.
Bioscientists are generally nature-lovers at heart, but the average bio lab is incredibly wasteful. Here are some ways to reduce your lab’s environmental impact.
An introduction to the Monash protein folding database, which contains protocols for refolding over 600 different proteins from inclusion bodies.
Could ligation independent cloning get any better? Well here is a technique that is sequence AND ligation independent.
Several peer-reviewed articles advocate using microwaves for sterilization, but would you trust microwave-sterilized growth medium?
If you have ever worked out the price of an electroporation cuvette you will realise that, at several dollars each, they are worth recycling. Accounts on how amenable electroporation cuvettes are to recycling vary, but I find that as long as you treat them well it is possible to use single cuvette many times. It’s…
Cover letters are possibly the most important documents you will write in your career because they can open or close the door to your dream job. But, surprisingly, people often under-estimate their importance and assume that they are just the lesser companion to the CV/resume. Of course, that’s not the case. Your cover letter should…
Only a handful of people ever become world experts in their field. The rest attain somewhere between a functional and world expert level of knowledge. So what makes the best better than the rest? Are they born with greater knowledge? Intelligence? Inner strength? Well, the latter is the more likely. Although some world experts are…
The eBook with top tips from our Researcher community.
