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…10 µL. 12. Reverse Pipetting Use this technique when pipetting viscous liquids or volatile solvents. Reverse pipetting also helps when pipetting ultra-micro samples of 0.5 µL or less. Push the piston down…
…in pipetting comes from dispensing the contents, ejecting the pipette tip, and keeping a tight grip on a poorly designed pipette. And pipetting is usually very repetitive, as any laboratory…
…system is your end-to-end automated pipetting solution for flexible pipetting, sample transfer, normalization, pooling, variable dilution, extraction and library preparation, etc. Complete Sample-to-Result Pipetting The MGISP-Smart 8 Automated Pipetting System…
…siRNA library screens, and drug treatment dilutions. However, I quickly learned that pipetting in these plates is more complicated than I thought. This article contains some practical hints to help…
…in you under-pipetting. The best practise is to aliquot your master mix to use reverse pipetting. This will pre-wet your pipette tip and help to pipette more accurately. Hopefully with…
…the lab must retrace their steps from time to time to sidestep the introduction of volumetric errors due to poor or inconsistent pipetting techniques. Want to avoid these experimental sinkholes? …
…helped me get out of that lab and into more interesting projects over the years. However, the importance of having a good technique for pipetting did not go without deliberation…
…on your own pipetting technique. It’s a very useful exercise to periodically measure your pipetting accuracy because this error is incorporated into every pipetting action you perform in the lab….
…pipetting schemes conveniently come in pipetting order, already reducing the chance of mis-pipetting. However, if this is not the case, I find it helpful to place my reagents on my…
…differences when pipetting. Note that you also need to make sure you are using a balance that has been properly calibrated too. How Pipetting Technique Affects Accuracy Before you check…
…microliters (really) to the 50 mL ones. On Saturdays, when there was almost nobody around apart from crazy Belarusian PhD students, my supervisor would furtively use mouth pipetting for her…
…the vessel’s cap/lid so I would recommend you pipetting any fluid that gets stuck back into the solution (slowly!) You can also try very gently pipetting with the dial turned…
…doing repetitive pipetting helps maintain my focus. But, I did notice that I stopped singing whenever extra attention was needed in my pipetting efforts. Doodling while listening, or singing while…
How often do you make errors in the lab that ruin a good experiment? Rather than flaws in experimental design, I mean errors like forgetting to add a reagent, pipetting…
…done, but this is not recommended as repeated pipetting may result in shearing of the DNA fragments affecting the size distribution. Again, proper pipetting skills are required to ensure that…
…to your pipetting technique. Ergonomic Tips Doing repetitive tasks, like pipetting, can cause damage to joints and result in repetitive stress injury (RSI). In light of this, companies have designed…
…Replication: Replicating your qPCR setup reduces the impact of poor pipetting. You’ll only use 1 ?l of your library in each reaction and any under- or over-pipetting can affect molarity…
…and Precision of Our Measurements? Understanding the concepts of accuracy (also known as trueness) and precision in a simple pipetting example is one matter, but it’s trickier to identify and…
…by pipetting or ideally using a plate vortex. 4. Keep Things Clean, Especially for RNA-seq Spend a little extra on RNase free and non-stick tubes, they won’t make a bad…
…prefer to avoid it. Simply because the extra pipetting you would use to mix the cells before taking a sample to count risks damaging the cells further and is likely…
…or glass plates can induce DC maturation and skewing to the adherent macrophage phenotype. Avoid glass, even when pipetting the DCs while changing the culture medium. Also, too much shearing,…
…you can do the whole thing in a cold room, even better! (A bit uncomfortable, but worth it!!) Treat the cells very gently – keep pipetting to a minimum and…
…pipetting. Plus this technique can also be used to extract DNA and RNA! A Brief Introduction Before we get down to business let me tell you about the important players…
…quit science because of this and became a castle curator. So how do you minimise the amount of one of the most repetitive tasks – pipetting – while maximising the…
…detergents can facilitate the solubilization and separation of your cellular components. Step 3: Collect Your Fraction of Interest Fractions are collected by pipetting through the high viscosity media gradients with Pasteur…
…be trained in radiation safety and have geared up to protect yourself, remember not to rush. Remain focused and take your time when handling and pipetting radioactive reagents. Rushing only…
Pipetting all day? Scrolling and scrolling through Excel columns trying to make sense of your data? Spending hours at the microscope because your boss wants Nature-worthy pictures? It’s not uncommon…
…fresh one without worrying about contaminating the master vial. 4. Use Pipettes Calibrated for Low Volumes If you require absolute accuracy in quantification, use pipettes calibrated for low volume pipetting…
…been (unintentionally) inoculated with slightly different numbers of cells initially owing to slight pipetting differences. We call this process variation. Second, there’s also variation that comes from the measurement itself,…
…Table 1 and explained below. Table.1 The Advantages and Disadvantages of One-step and Two-step RT-qPCR One-step RT-PCR Two-step RT-PCR Pros Fast Easier to control Reduced chance of pipetting errors…
…to detach cells from the dish and provides more volume for pipetting. To completely inactivate the detachment agent, collect the cells in a larger volume of growth media or BSS+FBS…
…when you pipette your beads, make sure to clean up any droplet hanging on the outside of your pipette tip. And, of course, make sure to use good pipetting practices,…
…protocol involves suspending cells in melted agarose and pipetting agarose and cell mixture into a gel mold before carrying out the DNA extraction. Extraction requires a series of washes to…
…their shelf life there are a few precautions you can take: Use sterile pipetting techniques whenever you work with your histology buffers. Store your buffer in the refrigerator. Make up…
…paper include: Subsampling error due to pipetting aliquots of your nucleic acid extract as input into the qPCR. Quality control of experiments, including internal controls to monitor PCR inhibition. Importance…
…phase (after 12-16 hours of growth). 2. Vortexing and pipetting your plasmid — take it easy! Nicked or linear DNA may occur due to mechanical shearing of DNA if preps…
…good pipetting skills are critical to get proper qPCR results. To account for pipetting errors, test DNA samples in triplicate. Steps to get Ready Planning is key Plan out your…
…the hood’s airflow 7. Keep Bottles and Plates Covered Simple enough. This helps your cultures remain contaminant-free! 8. Tilt Bottles and Tubes at a Slight Angle While Pipetting When pipetting…
…of technical artifacts, such as pipetting errors. It also provides us with a baseline (the ‘point zero’) against which to compare and look for differences between samples. …the exceptions There…
…still experiments that require meticulous and repeated pipetting. After all, those hundreds of tubes’ worth of competent cells aren’t going to divvy themselves up! Solution: To increase efficiency, consider using…
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