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ELISA: How I Wonder What You Are

…are many permutations of this assay (including an indirect ELISA described here), but I narrowed it down to two options: a direct ELISA or a sandwich ELISA. 1. Direct ELISA

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Tips For Better ELISA Results

…formats to choose from, depending on your research needs. These include direct ELISA, indirect ELISA, competitive ELISA and sandwich ELISA. We have previously covered the basic steps common to all…

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Crash Course: Developing a Fool-Proof ELISA

…tutorial, you will find: When and why you should use the different types of ELISAs- direct, indirect, sandwich, competitive/inhibition ELISA Major considerations for developing your ELISA Tried and tested tips…

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Troubleshooting a Faulty ELISA

…might be able to dilute out the interfering substance. Keep these tips in mind the next time your ELISA isn’t going to plan. For more reading on everything concerning ELISAs…

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Are Proteins Adsorbing to Your Labware?

…**While ELISAs are dependent on target proteins non-specifically binding to a plate, background from other proteins adsorbing to the plate can be an issue in direct capture ELISAs. ↩ Further…

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What’s THAT Doing in My Culture?

…more sophisticated equipment such as electron microscopy, immunostaining, ELISA, or PCR. Mycoplasma are estimated by some as the most common contaminant. Simplified bacteria, Mycoplasma are the smallest self-replicating organism known….

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Buying a Secondary Antibody: Why all the Choices?

…phosphatase. Incubation of the secondary antibodies with appropriate substrates can produce color or light for detection of the intended protein. Colorimetric assays are commonly used in immunohistochemistry or ELISAs while…

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The Seven Deadly Sins of HPLC

…you are quantifying, ELISA might be a better option if antibodies and/or commercial kits are available. Furthermore, due to retention shifts and variability between set-ups, it’s difficult to compare data…

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A Beginner’s Guide to Exosome Isolation

…combination of specific antibodies to capture different types of exosomes from your sample. We do this by immobilizing these antibodies on ELISA plates, magnetic and chromatography beads, or use them…

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Crash Course in Microbial Identification

…diagnosis and the timely initiation of proper antimicrobial therapy. Microarray-based platforms typically use similar barcode regions as other PCR-based methods (above). 8. Immunological Identification ELISA-based methods can be set up…

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