Search results for: elisa
Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA)
…suggests, PCR-ELISA combines an immunological method (ELISA) to detect and quantify specific PCR products directly after immobilization of DNA on a microtiter plate. PCR-ELISA is also less commonly known as…
Read MoreELISA: How I Wonder What You Are
…are many permutations of this assay (including an indirect ELISA described here), but I narrowed it down to two options: a direct ELISA or a sandwich ELISA. 1. Direct ELISA…
Read MoreLet me introduce you to ELISA…No, not the girl…The assay.
…background noise than if you used an indirect capture ELISA (see below). Capture Way 2: Indirect Capture ELISA In indirect capture ELISA, instead of capturing your entire complex mixture, only…
Read MoreRoadside Assistance: Fixing Your Broken-Down ELISA
…good ELISA results! ELISA in a Nutshell Whether you’re doing a direct ELISA, an indirect ELISA or a sandwich ELISA you will need to carry out a similar set of…
Read MoreTips For Better ELISA Results
…formats to choose from, depending on your research needs. These include direct ELISA, indirect ELISA, competitive ELISA and sandwich ELISA. We have previously covered the basic steps common to all…
Read MoreA New Frontier in Protein Quantitation: AlphaLISA
…how you can improve it? Let me guide you through the basics of ELISA, and introduce you to my favorite ELISA technique—AlphaLISA. First Things First… So, what is an ELISA?…
Read MoreCrash Course: Developing a Fool-Proof ELISA
…tutorial, you will find: When and why you should use the different types of ELISAs- direct, indirect, sandwich, competitive/inhibition ELISA Major considerations for developing your ELISA Tried and tested tips…
Read MoreTroubleshooting a Faulty ELISA
…might be able to dilute out the interfering substance. Keep these tips in mind the next time your ELISA isn’t going to plan. For more reading on everything concerning ELISAs…
Read MoreWestern Blot, ELISA, SPR, Biosensor Assay or PCR: Which Technique Should I Use?
…you want to investigate receptor-ligand binding, a biosensor assay might be the way to go. When Should You Use EMSA or ELISA? Using electrophoretic mobility shift assays (EMSA) or ELISA,…
Read MoreMultiplex Cytometric Bead Array: The ABCs of CBAs
…different fluorochrome (purple dot) will be used to detect the proteins. How Is CBA Technology Different from an ELISA? The ELISA is a very robust method for protein quantitation. It…
Read MoreFlow Cytometry Gone Viral: Introducing Flow Virometry
…Commonly used assays to detect virus particles include plaque assays and enzyme-linked immunosorbent assays (ELISA). Western blots are often used when we need to analyze the expression of the viral…
Read MoreAn Exploration of the Sigmoidal Curve – Math for the Rest of Us
ELISA (Enzyme-Linked Immunosorbent Assay) is the heartbeat of many labs in the research world, owing to its simplicity and its ability to answer a very basic question: how much of…
Read MoreIntroduction to Liquid Handling for NGS Workflows
Date: Thursday May 19th 2022 Available On Demand Speakers Elisa Viering Product Marketing Manager Elisa joined Eppendorf in 2021 as a Product Marketing Manager for automated Liquid Handling and PCR…
Read MoreA Primer on Checking the Methylation State of the Genome
…immunosorbant assay (ELISA) is a detection system based on target-specific antibodies. There are a lot of ELISA kits out in the market to look at global methylation pattern. This is…
Read MoreAre Proteins Adsorbing to Your Labware?
…**While ELISAs are dependent on target proteins non-specifically binding to a plate, background from other proteins adsorbing to the plate can be an issue in direct capture ELISAs. ↩ Further…
Read MoreHow Strong is Your Binding? A Quick Introduction to Isothermal Titration Calorimetry
…enzyme-linked immunosorbent assay (ELISA), etc.]. This means there’s no need to worry about tags or immobilization surfaces obscuring biologically relevant interactions. Secondly, affinities from nanomolar through to millimolar can (in…
Read MoreWhat’s THAT Doing in My Culture?
…more sophisticated equipment such as electron microscopy, immunostaining, ELISA, or PCR. Mycoplasma are estimated by some as the most common contaminant. Simplified bacteria, Mycoplasma are the smallest self-replicating organism known….
Read MoreBrefeldin A v Monensin: How to Hunt for Proteins
…In many circumstances, the quantity of the secreted protein, and thus the level of cellular activation can be assessed by ELISA. However, if you are limited by the amount of…
Read MoreTips for Choosing and Using a New Primary Antibody
…antibody is not sensitive enough to detect protein expressed at a physiological level. If you are doing ELISA, look for ELISA-specific paired antibodies matched by species and antibody structure to…
Read MoreBuying a Secondary Antibody: Why all the Choices?
…phosphatase. Incubation of the secondary antibodies with appropriate substrates can produce color or light for detection of the intended protein. Colorimetric assays are commonly used in immunohistochemistry or ELISAs while…
Read MoreSeven Things That Really Annoy Me About Tissue Culture
…for western blot analysis, ELISA… the list goes on. It is also one of the places in which scientists tend to butt heads. An improperly functioning tissue culture facility can…
Read MoreProtein Lysate TLC: Pro-Tips to Keep Your Protein Extracts in (Experimentally) Perfect Shape.
…have full protection. From protein immunoblots to ELISA to functional assays, the most important thing in protein biochemistry is high-quality proteins. If you handle your tissue and cell extracts with…
Read MoreFinding a Needle in a Needlestack Using Phage Display
…to your protein-of-interest, map binding epitopes between two proteins, or generate useful monoclonal antibodies for use in Western blots, ELISA, or other immunoassays. Not bad…not bad at all. How Phage…
Read MoreAliens from Outerspace: Navigating a Lab in the West
…use the resources you have in the form of more experienced students, and a knowledgeable supervisor. Perfect example: the first time I did an ELISA, my boss asked me to…
Read MoreHow to Approach your First Experiments as a New PhD Student
…blot, ELISA or mass spectrometry and gene expression by microarray, qPCR or northern blot. The more evidence you can collate to prove your point, the better. Essentially it’s like another…
Read MoreThe Seven Deadly Sins of HPLC
…you are quantifying, ELISA might be a better option if antibodies and/or commercial kits are available. Furthermore, due to retention shifts and variability between set-ups, it’s difficult to compare data…
Read MoreFive Ways Science Outreach Can Benefit Your Benchwork!
…Passionately in love. Now, you sort of want to set it on fire – like when your plate washer goes haywire on your expensive ELISA. Do what some couples do…
Read MoreSuccessful Start: Tips for Establishing Organoids
…by rtPCR or western blot as early as any other tissue sample. ELISA can be carried out on the surrounding media and organoids can be mounted, sliced and stained for…
Read MoreFluorescent Western Blotting: Lowdown and Advantages
…by electrophoresis followed by western blotting may offer more precision than other antibody-based detection assays (e.g., ELISA). This is because even if the primary or secondary blotting antibodies cross react…
Read MoreHow Pure is Your Cell Culture Broth? Comparing Mycoplasma Detection Kits
…techniques including Hoechst staining, ELISA, or determining host biomarkers can alert you to the presence of a mycoplasma infection, most current kits rely on PCR. For example: The LookOut® Mycoplasma…
Read MoreAntibodies 101: Every Body Needs an Anti-Body!
…and target proteins as well as for drug delivery. Protein identification is the most common use of antibodies in molecular biology research labs. For example, Western blots and ELISA assays…
Read MoreA Beginner’s Guide to Exosome Isolation
…combination of specific antibodies to capture different types of exosomes from your sample. We do this by immobilizing these antibodies on ELISA plates, magnetic and chromatography beads, or use them…
Read MoreGetting Sensitive: Diagnostic Sensitivity and Specificity Simplified
…to compare a new qPCR assay to one currently in use or a qPCR to an ELISA. And if math isn’t your thing, there are a range of free on-line…
Read MoreA Biologist’s Guide to Choosing Your Fluorophore Palette
…Photographer: Simon Caulton. Rhodamine and Cyanine are a useful family of dyes used for fluorescence microscopy, flow cytometry, and ELISA. A few notable examples of the derivative rhodamine dyes are…
Read MoreTop 10 websites to help you with your PCR experiments
…hybridization probes for use in PCR-ELISA and other probe-based applications. 2. National Centre for Biotechnology Information (NCBI) Did you know that the NCBI website can be used for so much more…
Read MoreLearn-omics! What is that “Omics” I keep Stumbling Upon?
…that are focused on studying the proteins of an organism or the proteome. Technologies used: Enzyme-linked immunosorbent assay (ELISA), mass spectrometric immunoassay (MSIA) Metabolomics The technologies that are focused on…
Read MoreHelpful Tips Before Your First Rho Pull-Down Assay
…to stimuli, cells increase their Rho-GTP levels, which you can monitor via quantifying the level of Rho-GTP using an ELISA or Western blot. What Is a Rho Pull-Down Assay? A…
Read MoreThinking Outside the Box: Microscopy for Immunologists
…list goes on.). You may also think of a slew of assays that almost exclusively “belong” to immunologists, including ELISA, ELISpot, Flow Cytometry, chromium release assay, and so on. While…
Read MoreCrash Course in Microbial Identification
…diagnosis and the timely initiation of proper antimicrobial therapy. Microarray-based platforms typically use similar barcode regions as other PCR-based methods (above). 8. Immunological Identification ELISA-based methods can be set up…
Read MoreHow to Obtain Stellar Staining with Fluorescent IHC
…its specificity for your antigen of interest and its suitability for IHC or immunocytochemistry. Not all primary antibodies are created equal. Antibodies that are designed for western blot or ELISA…
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