Latest Events
Linking 2D and 3D Spatial Biology for Multiscale Mapping of Complex Tissues
Using Empower™ CDS Efficiently – 3 Key Optimization Areas
Illuminating Nanomaterial–Cell Interactions by Confocal Microscopy
Effortless ELISAs: Streamline Assays with Walkaway Automation
Innovations and Emerging Tools in Peptide Science
Empower™ CDS Onboarding: How to Train New Users Effectively
On Demand Events
Latest Articles
Overcoming Challenging Targets: What To Do When Midpoint CTE Returns Inconclusive Results
Midpoint CETSA is a common method for assessing target engagement by measuring protein stability at a single temperature, but it can obscure important mechanistic differences in complex proteins. This article explains how multi-temperature CETSA profiles reveal conformational state-specific binding, improving compound ranking and selectivity assessment. It highlights when to move beyond midpoint CETSA, especially for covalent mechanisms, mutant selectivity, and structurally diverse compounds, providing a practical decision framework for drug discovery.
Latest Podcasts
Choosing the right filter plate starts with understanding your assay biology. What must happen at the membrane determines everything that follows. Choose filter plates according to membrane chemistry (binding vs non-binding) and the correct pore size for your target (cells, proteins, or small molecules). Finally, ensure plate architecture and automation compatibility support consistent flow, minimal crosstalk, and reproducible results at scale.
qPCR Troubleshooting involves identifying and resolving common issues such as contamination, pipetting variability, reagent integrity, and reaction setup errors. This guide provides a clear framework to diagnose symptoms, optimize conditions systematically, and ensure reliable, reproducible qPCR results. It emphasizes the importance of proper primer handling, cycling conditions, and normalization for trustworthy data interpretation.
Choosing between qPCR or RNA-seq often means balancing speed and scope. qPCR is quick but limited to a small set of pre-selected genes, while RNA-seq offers a full transcriptome view but you typically wait weeks to get results. This article explores how 3′ RNA-seq bridges that gap, delivering genome-wide insights in days.
qPCR probes are fluorescently labeled oligonucleotides that provide sequence-specific detection during PCR, improving specificity over intercalating dyes. They use FRET-based quenching and signal release, enabling multiplexing and accurate target quantification. Advances in quencher chemistry have enhanced probe performance, while alternative methods like Plexor offer distinct detection mechanisms. Understanding these differences helps researchers select and optimize qPCR probes for reliable and precise molecular assays.
Microscopy & Imaging
Optical microscopy has always been restricted by the diffraction of light. Because conventional widefield and confocal microscopes cannot resolve structures substantially smaller than about 200nm laterally, they miss critical signaling compartments and nanoscopic organization. Newer super-resolution technologies, such as stimulated emission depletion (STED), structured illumination microscopy (SIM), and photoactivated localization microscopy (PALM), solve this problem…
DNA / RNA Manipulation & Analysis
Unsure which next-generation sequencing (NGS) method to choose for your cancer and precision medicine research? Learn about the latest tools and tailored next-generation sequencing solutions for cancer research in this short guide.
How-To Guides
Article Categories
- Analytical Chemistry & Chromatography Techniques
- Cells & Model Organisms
- Chemistry for Biologists
- Cloning & Expression
- DNA / RNA Manipulation & Analysis
- Flow Cytometry
- Genomics & Epigenetics
- Microscopy & Imaging
- PCR, qPCR & qRT-PCR
- Bioscience Mastery
- Basic Lab Skills & Know-how
- Career Development & Networking
- Dealing with Fellow Scientists
- Equipment Mastery & Hacks
- Getting Funded
- Lab Safety
- Lab Statistics & Math
- Organization & Productivity
