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Plasmid archiving at Addgene

Addgene is a non-profit plasmid repository that stores and distributes plasmids for academic labs. It's great if you work in an academic lab and they happen to have your plasmid - drop them an order and get...
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An Attractive Genomic DNA Isolation Kit

I was becoming a bit bored with the tedium of column-based kits, so when I had to isolate genomic DNA from a range of micro-organisms for a recent project I decided to try something new. Invitrogen's...
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You Know You’ve Been In the Lab Too Long When…

This has been doing the rounds all over the web, so I thought I'd post my 10 favorites. You know you've worked in the lab too long when... You wash your hands before you go to the toilet You tell your...
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Ethidium Bromide: A Reality Check

The hysteria among molecular biologists about our old friend ethidium bromide has long been an irritation to me. Researchers are rightly wary of this potential carcinogen. More recently this wariness has been...
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Protein Expression with a Cherry on Top

If you do a lot of heterologous protein expression, take a look at Eurogentec's CherryExpress Kit. Based on the T7 expression system, the CherryExpress vector has a sequence encoding a small red polypeptide...
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Choosing a Competent E.coli Strain

Of all the of competent E. coli cell strains available, which one should you choose? The choice of strain to use in a given experiment is determined in large part by the nature of the experiment and the set of...
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The World’s Fastest Miniprep?

I love this great time-lapse video entitled "a day in the lab". If only minipreps were this fast and easy! [youtube:http://www.youtube.com/watch?v=XKF_bItwkjk]
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Competent E.coli: To buy or not to buy?

Buying competent cells from commercial suppliers is convenient, provides a guarantee of quality and gives access to strains with a variety of in-built traits that assist with things like maintenance of...
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15 Reasons to Be a Scientist

Just for fun, here my top 15 reasons for being a scientist. Add your own reasons in the comments below if you so wish. 1. Not being stuck behind a desk all day every day 2. Conferences... see the world...
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One Tube PCR Cloning Method

I love shortcuts, and this one is very good. Chun-Ming Liu of Plant Research International has a number of molecular biology protocols on his website,  but my favourite is his One Tube PCR Cloning Method. The...
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