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How to Interpret IC50 and Kd: What Each Measurement Really Tells You (and Why They’re Different)

IC₅₀ and Kd are widely used in drug discovery, but they answer different questions. This article explains how to interpret IC₅₀ and Kd correctly, why they’re not directly comparable, and how combining both metrics leads to better compound prioritization and decision-making.

Expansion Microscopy: Achieving Nanoscale Resolution Using Conventional Microscopes

For decades, the diffraction of light has placed a hard limit on optical microscopy. Conventional widefield and confocal microscopes can’t resolve features smaller than about 200 nm laterally, which means critical signaling compartments and the nanoscopic organization of synapse-associated molecules remain hidden. The 21st century brought revolutionary super-resolution technologies, such as stimulated emission depletion (STED),…

How To Optimize Bioluminescence Assays for High-sensitivity Detection

To generate reliable quantitative bioluminescent data, you need more than a strong luciferase signal. Optimize bioluminescence assays for high-sensitivity detection by controlling substrate handling, timing, plate-reader settings, and normalization so technical noise doesn’t mask real biology. From luciferase and substrate choice to reader optimisation and in vivo bioluminescent imaging workflows, these practical strategies help you produce reproducible, interpretable data you can trust.

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How to Detect Low-affinity Protein Binding Without Surface Plasmon Resonance

Find out how to detect low-affinity protein binding without using SPR. The Octet® R8e BLI system bridges the gap between speed, simplicity, and sensitivity, transforming drug discovery workflows.


Microscopy & Imaging

Expansion Microscopy: Achieving Nanoscale Resolution Using Conventional Microscopes
Expansion Microscopy: Achieving Nanoscale Resolution Using Conventional Microscopes

For decades, the diffraction of light has placed a hard limit on optical microscopy. Conventional widefield and confocal microscopes can’t resolve features smaller than about 200 nm laterally, which means critical signaling compartments and the nanoscopic organization of synapse-associated molecules remain hidden. The 21st century brought revolutionary super-resolution technologies, such as stimulated emission depletion (STED),…


DNA / RNA Manipulation & Analysis


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10 Things Every Molecular Biologist Should Know

The eBook with top tips from our Researcher community.