Techniques / Microscopy & Imaging

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Article

A Beginner’s Guide to Haematoxylin and Eosin Staining

Once a tissue specimen has been processed by a histology lab, and transferred onto a glass slide, it needs to be appropriately stained for microscopic evaluation. This is because unstained tissue lacks...
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Article

Free-Floating Versus Slide-Mounted Sections for Immunohistochemistry

After countless immunos with free-floating sections – troubleshooting, testing antibodies, and finally doing the actual experiments – I felt like an expert on immunohistochemistry. I knew everything there...
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Article

Spectral Unmixing in Fluorescence Microscopy

When we are doing a double labelling, it makes good sense to choose two fluorophores with spectra widely spaced apart to avoid mixing. Why combine GFP with Rhodamine, when you can combine it with Texas Red?
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Article

Multifocal Structured Illumination Microscopy: The Fast Food of Super-Resolution Techniques

While most of us have heard of super resolution microscopy, many of you may not have heard of MSIM, or Multifocal Structured Illumination Microscopy. This under-the-radar imaging technique is relatively quick,...
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Article

Resolving your Noise Complaints – the Basics of Signal/Noise Ratio in Microscopy

The resolution of any microscope is related to the numerical aperture of the lens and the wavelength of light used to form the image, and can be calculated using Abbe’s law (read more about Abbe’s law...
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Article

What Lipid Bilayers Can Do For You

We have to rely on artificial systems to test our hypotheses and often have to come up with original set-ups to investigate specific problems. One of these creative inventions is the use of supported lipid bilayers.
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Article

The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each...
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Article

Bright Minds: Overcoming Autofluorescence in Human Brain Samples

The human brain autofluoresces - a funny thought next time you see a cartoon character with a bright idea and a light bulb over his head - but not so funny if you are attempting immunofluorescence analysis. But...
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Article

Immunoscience or Immunoalchemy?

First of all let me say the technique of labeling tissues (immunohistochemistry, IHC), and cells (immunocytochemistry, ICC) is indeed immunoscience NOT alchemy, though at times it may certainly seem like...
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Article

Getting Started with Raman Spectroscopy: What You Need to Know

Are you an assiduous biologist who prefers label-free imaging methods for biological samples analysis? Raman spectroscopy offers you a wonderland of imaging technique with unlimited benefits. To start with,...
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