Techniques / Microscopy & Imaging

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Article

Microscope Cameras: From SLR to CMOS Devices

Photography has undergone great improvements in the last few decades. In times gone past, photographic film was used. Now most researchers use digital means to capture their images. But not all digital cameras...
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Article

Immunohistochemistry: Getting The Stain You Want

Immunohistochemistry (IHC) is a vital tool, not just at your wet bench but also in clinical labs the world over. IHC is used extensively in hospitals and veterinary practices in grading and studying cancers,...
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Article

How to Make Lipid Bilayers

Supported lipid bilayers are a very useful tool in many fields of cell and membrane biology. But how easy is it to make them? Bilayers can be made quite reproducibly, once you have found a reliable protocol!...
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Article

A Beginner’s Guide to Haematoxylin and Eosin Staining

Once a tissue specimen has been processed by a histology lab, and transferred onto a glass slide, it needs to be appropriately stained for microscopic evaluation. This is because unstained tissue lacks...
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Article

Free-Floating Versus Slide-Mounted Sections for Immunohistochemistry

After countless immunos with free-floating sections – troubleshooting, testing antibodies, and finally doing the actual experiments – I felt like an expert on immunohistochemistry. I knew everything there...
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Article

Spectral Unmixing in Fluorescence Microscopy

When we are doing a double labelling, it makes good sense to choose two fluorophores with spectra widely spaced apart to avoid mixing. Why combine GFP with Rhodamine, when you can combine it with Texas Red?
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Article

Multifocal Structured Illumination Microscopy: The Fast Food of Super-Resolution Techniques

While most of us have heard of super resolution microscopy, many of you may not have heard of MSIM, or Multifocal Structured Illumination Microscopy. This under-the-radar imaging technique is relatively quick,...
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Article

Resolving your Noise Complaints – the Basics of Signal/Noise Ratio in Microscopy

The resolution of any microscope is related to the numerical aperture of the lens and the wavelength of light used to form the image, and can be calculated using Abbe’s law (read more about Abbe’s law...
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Article

What Lipid Bilayers Can Do For You

We have to rely on artificial systems to test our hypotheses and often have to come up with original set-ups to investigate specific problems. One of these creative inventions is the use of supported lipid bilayers.
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Article

The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each...
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