Techniques / Genomics & Epigenetics

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Article

Where Did It All Go Wrong?! Quality Control For Your NGS Data

You’ve carefully collected your samples, extracted nucleic acids and made your first set of next-generation sequencing libraries. How are you going to know if the data you get back is any good and whether it...
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Article

Breaking Down the Assembly of Nucleic Acid Sequences

Microbiome—a term that has become a hot topic in recent years—has scientists of all disciplines wanting to know more. Microbes are everywhere. On any type of surface you can think of. Our physical make up,...
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Article

Anchored Multiplex PCR for Next Generation Sequencing

Anchored multiplex PCR (AMP) is a powerful method for amplifying minuscule amount of nucleic acids. Combined with Next Generation sequencing, AMP just might be what you need to identify genetic mutations.
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Article

Kiss your samples goodbye: Outsourcing your Next-Gen experiment

Genomic Science has come a long way since the early days of Sanger sequencing in the 1970’s. Today, there are jazzy new sequencing technologies that include fragment analysis, epigenetic sequencing,...
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Article

Sanger Sequencing: How the Genome Was Won

I’ve never run a sequencing gel in my life, but people around me did, and they spent a lot of time on getting it just right. Although the principle described by Sanger in 1975 sounds straightforward (1),...
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Article

Navigating the Sea of Human Genetics, Part I

Ahoy mateys! At my workplace we've recently become EXTREMELY excited about a resource called the HuGE Navigator. It's so great that it's going to take me a couple of articles to highlight its features. Hop on...
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Article

Get Your Single Nucleotide Polymorphisms Straight From the Oven!

While it is true that there are some useful websites like SNPedia, or NCBI that can help you find rs codes for genetic variants, sometimes you need that info coming straight from the oven – particularly when...
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Article

Using a Genome Viewer to Scope your Sequencing Data

Now you've got great sequencing results, thanks to Nick's article on improving sequencing results. Now what? Well now you need some software (preferably free) to analyze your data. BioEdit is a good...
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Article

Get Started in Genome Editing with CRISPR

What was once a dream is now reality: You can now target and selectively edit the genome, thanks to the CRISPR/Cas9 technology. Changing forever how we approach biological questions. CRISPR/Cas9 is pretty...
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Article

Want NGS Success? Consider These Three Factors

NGS is still something a little scary for most operators. Mainly because of the price, which can make the pipette, in the hand of the best of us, shake a little with fear. And even though the protocols have...
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