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Yevgeniy Grigoryev

Yevgeniy has a PhD in Systems genomics, immunology, molecular biology from Scripps Research. He is currently a lecturer at the City College of New York and a freelance science writer specializing in communicating science to diverse audiences. My extensive background in scientific research, science writing and journalism allows me to translate various scientific topics into clear, concise, and accurate language that appeals to a general audience. As I science journalist with Nature Medicine, I honed my skills as an effective communicator and writer, interacting with many scientists across many different fields, including policymakers, clinicians, and technology experts. Moreover, my experience as a science writer with the BiteSizeBio online scientific community allows me to promote and encourage communication among different sectors of the scientific community and cultivate new relationships within the community.

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Articles by Yevgeniy Grigoryev

Image of blurred light to represent signals from DNA microarrays

Introduction to DNA Microarrays

By Yevgeniy Grigoryev | October 22, 2021

This is the first installment in the DNA microarray series where I will introduce the technology and explain the basics.

An image of two lengths of copper wire, one of which is being cut with wire cutters by an electrician, to represent alternative splicing

What is Alternative Splicing, and Why Is It Important?

By Yevgeniy Grigoryev | September 17, 2021

Need to brush up on your alternative splicing knowledge? We’re here to help with our guide to this splicing mechanism.

Image of a child counting using an abacus denoting the ease of counting cells using a hemocytometer

Cell Counting with a Hemocytometer: As easy as 1, 2, 3…

By Yevgeniy Grigoryev | June 29, 2021

Do hemocytometers look scary and complicated with their multiple tiny squares, boxes, and grids? Take a look at our article and see how easy it actually is to use a hemocytometer.

Interview Techniques: Interview like a STAR

Interview Techniques: Interview like a STAR

By Yevgeniy Grigoryev | October 14, 2013

During your time interviewing for different jobs, more likely than not you will encounter employers who conduct behavioral interviews. What is a behavioral-based interview, you may ask? Behavioral interviewing is supposed to uncover your past job-related behavior to predict how you will behave in the future. It is based on the assumption that your past…

Mycoplasma: The Hidden Anarchist of Cell Culture

By Yevgeniy Grigoryev | September 4, 2013

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until their numbers swell and then they attack in strength, overwhelming their victims before they can put up a fight! What is…

5 Ways to Really Screw Up Your RNA Prep

By Yevgeniy Grigoryev | July 29, 2013

Unlike DNA, which can last for eons, RNA is a fragile and degradation-prone cousin. After working with RNA for a while, one becomes quite paranoid about handling RNA because even a single sneeze or drop of saliva can potentially affect your results. The reason is that there are enzymes called RNases that specifically target and…

Crush Like an Elephant, Soak Like the Rain: Old-School DNA Gel Extraction

By Yevgeniy Grigoryev | May 7, 2013

In my previous article on DNA gel extraction, I explained how most commercially available DNA gel extraction kits work. However, there was a time before our society was blessed with these convenient marvels of technology and scientists had to summon the gods of “Crush and Soak”. This method has been proven for millennia, as people…

How DNA Gel Extraction Works

By Yevgeniy Grigoryev | May 1, 2013

Isolating pure DNA is key to many downstream applications for molecular biologists.  Isolating large quantities of pure DNA used to be a laborious task.  But thanks to commercially available kits, older methods have been streamlined to allow efficient recovery of pure DNA. In this article, I will talk about a method called DNA gel extraction,…

An image of cells to depict free PCR

The A-Z List of Things that go “Missing” in the Lab

By Yevgeniy Grigoryev | May 1, 2013

Here is a fun list of things that you are most likely to lose to light-fingered colleagues or nocturnal ghosts of academia. The emphasis here is on fun, so as disclaimers often go, if your experience proved somewhat different, this list “does not represent the actions of every individual or ghost who you might encounter…

An graphic of expanding circles to depict a method for cheaper bacterial transformation.

Blast your way to quicker, cheaper bacterial transformations

By Yevgeniy Grigoryev | April 8, 2013

If you want a more efficient, cheaper way to do bacterial transformation, you are definitely going to like this article.

Isolation of total RNA, including microRNA, from cells and tissues

By Yevgeniy Grigoryev | March 19, 2013

Whereas DNA can survive for millennia, RNA is short-lived, which is a bummer if you are trying to isolate it. The reason – is that RNA is prone to degradation by enzymes called RNases. Therefore, isolation of total RNA from cells and tissues requires a method that will efficiently isolate the RNA from the samples…

Outgrown the Roost: Passaging Suspension Cells

By Yevgeniy Grigoryev | February 6, 2013

Previously, you have learned about passaging adherent cells and read a quick protocol to make it happen. In this article, I will talk about passaging suspension cells. Some cells naturally live in suspension in body fluids and do not attach to surfaces, such as cells of hematopoietic origin found in our bloodstream. Culturing these suspension…

miRNAs from Plasma and Serum

Top Tricks for Isolation of miRNAs from Plasma and Serum

By Yevgeniy Grigoryev | February 5, 2013

MicroRNAs (miRNA) are short, non-coding RNAs involved in post-transcriptional silencing of gene expression. miRNAs can be associated with exosomes and can function as cancer-specific biomarkers. This, coupled with the fact that they are stable in plasma and serum makes them valuable diagnostic tools, as long as they can be reliably isolated from the serum and…

How Can A Single Mutation Affect Splicing Regulation?

How Can A Single Mutation Affect Splicing Regulation?

By Yevgeniy Grigoryev | January 25, 2013

Alternative splicing is a highly orchestrated process that uses a multitude of regulatory mechanisms. Splicing specificity involves a precise interaction between cis- and trans-acting regulatory elements, and factors that disrupt these interactions can result in aberrant splicing. There are multiple ways in which mutations can affect splicing fidelity: A point mutation in the cis-acting splice…

How to Detect Alternative Splicing Variants

How to Detect Alternative Splicing Variants

By Yevgeniy Grigoryev | January 11, 2013

Alternative splicing events often occur in a spatiotemporal manner, and some are regulated by alternative splicing regulators, with striking variation across tissue types and developmental stages. Alternative splicing events are often differentially regulated across tissues and during development, as well as among individuals and populations, suggesting that individual isoforms may serve specific spatial or temporal…

Principles and Mechanisms of Mammalian Cell Transfection

Principles and Mechanisms of Mammalian Cell Transfection

By Yevgeniy Grigoryev | September 5, 2012

Mammalian cell transfection is a technique commonly used to express exogenous DNA or RNA in a host cell line (for example, for generating RNAi probes). There are many different ways to transfect mammalian cells, depending on the cell line characteristics, desired effect, and downstream applications. In this article, I will review the different methods of…

An image of lab furniture to depict how not to wreck your autoclave.

Book Review: “What Color Is Your Parachute?”, by Richard Nelson Bolles

By Yevgeniy Grigoryev | August 20, 2012

“The clearer your vision of what you seek, the closer you are to finding it. For what you are seeking is also seeking you.” Finding a job or a career could feel very much like falling though a void. “What color is your parachute?” by Richard Nelson Bolles is as much a practical guide to…

How to Pursue a Non-Research Career While in Graduate School

How to Pursue a Non-Research Career While in Graduate School

By Yevgeniy Grigoryev | July 27, 2012

‘Would you tell me, please, which way I ought to go from here?’ ‘That depends a good deal on where you want to get to,’ said the Cat. ‘I don’t much care where,’ said Alice. ‘Then it doesn’t matter which way you go,’ said the Cat.                                    From Alice’s Adventures in Wonderland. According to a…

What Has Methylation Done For You Lately?

What Has Methylation Done For You Lately?

By Yevgeniy Grigoryev | April 18, 2012

Epigenetics is the study of heritable changes in the phenotype of a cell or an organism that are not encoded by the genome (hence epi which means ‘above’ in Greek, and genetikos which means ‘origin’). In this article, we’ll discuss DNA methylation, a common epigenetic modification: what it is, how to detect it, and how…

How To Use Your Department To The Fullest

How To Use Your Department To The Fullest

By Yevgeniy Grigoryev | March 21, 2012

“No one can whistle a symphony.  It takes a whole orchestra to play it.” – H.E. Luccock In my previous article on “Starting your PhD the right way”, I already mentioned the importance of using your department’s resources to your advantage. In this article, I will expand on how to use your department to the…

An image of colors to depict care for your pH meter.

How Much Information is Stored in the Human Genome?

By Yevgeniy Grigoryev | March 16, 2012

The other day I was having a conversation with a friend of mine who had some background in computer science. The conversation shifted towards my research and the following question came up: What is the amount of digital information stored in a human genome? I started searching in the deep dark corners of my brain,…

How To Start Your PhD The Right Way

How To Start Your PhD The Right Way

By Yevgeniy Grigoryev | February 24, 2012

You have just finished your undergraduate studies, or perhaps you have been working in the field for a while, you are full of energy and zest and you want to prove to the world that you are carved from the right stone to pursue PhD studies. Going to graduate school is a very important, potentially…

When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

By Yevgeniy Grigoryev | January 4, 2012

RNA interference (RNAi) may have originated as a defense mechanism to protect cells against foreign genes introduced by viruses. This concept has since been put to use to create a powerful experimental tool for investigating gene function in organisms. Small-interfering RNA (siRNA) libraries for investigating genome-wide function can be produced by chemical synthesis of probes…

Tango Therapy

Tango Therapy

By Yevgeniy Grigoryev | October 28, 2011

“While I dance I can not judge, I can not hate, I can not separate myself from life. I can only be joyful and whole. This is why I dance.” – Hans Bos While in graduate school, I tried really hard to find an activity that would allow me to disassociate my body from my…

How DNA Microarrays are Built

How DNA Microarrays are Built

By Yevgeniy Grigoryev | September 5, 2011

Previously I introduced the DNA microarray technology and described the principle behind it: hybridization between the nucleic acid sequence from the biological samples being examined and a synthetic probe immobilized and spatially arrayed on a solid surface, the microarray. In this article, I will explain how these probes are designed and positioned on the array. I…

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