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Few technical breakthroughs have changed the face of their field like the Polymerase Chain Reaction (PCR). Gene cloning, sequencing of complex genomes, DNA fingerprinting and DNA-based diagnostics are just some of the techniques that were either inefficient, crude or plain impossible before PCR. The technique has revolutionized biological research and biotechnology to such an extent…
Talented, enthusiastic scientist required. Must have good organizational skills.Do you fit the bill? With the pace of molecular biology and biochemical research quickening year on year, the importance of good organization and planning skills for researchers is becoming increasingly important. Here are 5 ways to ensure that your data is organized and easily analyzed, samples…
When heterologous gene expression goes wrong it can be a real headache. Here’s my checklist for the steps to take when you encounter problems with this dark art. 1. Check the construct by sequencing the expression cassette to make sure that everything is as you expect. A lack of expression could result from a stray…
This has been doing the rounds all over the web, so I thought I’d post my 10 favorites. You know you’ve worked in the lab too long when… …any more?
Of all the competent E. coli cell strains available (including both chemically competent or electrocompetent E. coli), which one should you choose? The choice of strain to use in a given experiment is determined in large part by the nature of the experiment and the set of traits that best fit it. In this article…
Buying competent cells from commercial suppliers is convenient, provides a guarantee of quality, and gives access to strains with a variety of in-built traits that assist with things like maintenance of plasmid integrity (more on these traits later). However, this can be an expensive business. Alternatively, competent cells of any strain, including the specially-constructed commercial…
Just for fun, here my top 15 reasons for being a scientist. Add your own reasons in the comments below if you so wish. 1. Not being stuck behind a desk all day every day 2. Conferences… see the world for free 3. Understanding some of the fundamentals of life and the universe 4. Getting…
I have been at a conference today and don’t have too much time to write this, so this will be a quick article. After watching lots of speakers of varying competence, I thought that it would be good to outline some tips for great presentations. Speaking is an integral part of a scientist’s job, and…
I’ve put together this list of 10 pieces of free molecular biology software for Macs. I hope you will find at least some of it useful. If any of your favorite free programs are not included, please email me and I’ll add them or you can leave a comment with a link. If you are…
Isn’t it a pain digesting, purifying and dephosphorylating your cloning vector prep to eliminate prevent high background in your ligation/transformation? A new generation of positive selection cloning vectors promises to eliminate all of that hassle by killing off any vector that has not taken up the insert you are trying to clone. Positive selection cloning…
Artificial gene synthesis was first reported in 1972 when a group of researchers at Massachusetts Institute of Technology synthesized a complete yeast alanine tRNA gene. Synthesis of the first peptide- and protein-encoding genes ensued in the following decade. Since then, synthetic biology has advanced in leaps and bounds, and custom gene synthesis, a one-time expensive option for…
Get awesome pictures of your DNA gels with a standard digital camera and an orange filter. Here’s how.
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