Techniques / DNA / RNA Manipulation and Analysis

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A Quick Guide to Screening by Restriction Digestion

PCR screening is great for identifying potential positive clones after cloning/subcloning. However, you’ll still want to confirm that the DNA sequence is correct for any positives identified. The easiest and...
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Article

Polysome Profiles: What You Need to Know

Has your supervisor asked you to do a polysome profile? Are you attempting them but running in to problems? This article will help explain what they are, the basics of the protocol and provide some helpful tips...
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Antibiotics Used in Molecular Biology

Antibiotics are used in a wide range of techniques in molecular biology. My aim with this post is to provide an easy reference to some of the main ones used in molecular biology, their mechanisms, range and...
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E.coli Electroporation vs Chemical Transformation

This is the first in a three part series on the transformation of E.coli. By the end of this you should be an expert on E.coli transformation and on which strains to choose for different applications. If you're...
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Article

A Guide to Solid Phase Reversible Immobilization

  Scientists today depend heavily on many molecular biology techniques to perform their research. For example, with the advent of next generation sequencing (NGS): scientists are able to look at very...
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The Basics: How Phenol Extraction of DNA Works

Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation. It's commonly used, but not commonly...
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5 Tips on Vector Preparation for Gene Cloning

One of the most crucial steps in any cloning procedure is the preparation of the vector. Get it wrong and your chances of success will be drastically reduced. The overall aim for a good vector preparation is to...
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5 DNA Ligation Tips

DNA ligations can be frustrating. Sometimes they just don't work, for no obvious reason. My top 5 DNA ligation tips will help to improve the efficiency of your ligation reactions and hopefully give a better...
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Get Ready, Get Set, Retro – How to Get Started With Retroviral Transduction

Retroviral transduction is becoming a popular choice for gene delivery into mammalian cells and has multiple advantages over other techniques. If you decide to start work on this useful technique, here is...
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Article

CRISPR Genome Editing – What You Need to Know to Get Started

I’m sure that anyone in the world of biology has heard of CRISPR-Cas genome editing by now, but I’m hoping to give you some ideas on what CRISPR-Cas can do for you and what using it involves.
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