Techniques / DNA / RNA Manipulation and Analysis

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Eight Top Tips to Maximize Yield from Whole Blood DNA Isolation

When you perform genomic DNA extraction from whole blood, low yield or low quality DNA can result in many issues. No matter your intended downstream application—qPCR, next generation sequencing, Sanger...
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Article

Essential Factors for Successful Gateway Cloning

An efficient cloning system that is in vogue at present is the Gateway® Cloning Technology. Gateway cloning gives researchers the opportunity to easily transfer DNA fragments into plasmids using proprietary...
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Article

Demystifying REBASE and NEBcutter

Two years ago, all I knew was third BASE in my baseball field and the cutter ball from the pitcher. Now, I know a lot more about lab-based BASES and cutters: REBASE and NEBcutter. While they sound like baseball...
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Article

How to Make Your Own Chemically-Competent Cells

I once had the terrible experience of not being able to run an assay because I ran out of commercial stock of transformation-competent Escherichia coli (E.coli). From that day, I learned to make my own...
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Article

How to Improve Plasmid Yield Using Antibiotics

After the initial excitement of growing and isolating plasmid DNA, routinely preparing plasmid mini/midi/maxi preps gets boring. You definitely want a way to squeeze the maximum amount of plasmid DNA out of...
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A Quick Guide to Screening by Restriction Digestion

PCR screening is great for identifying potential positive clones after cloning/subcloning. However, you’ll still want to confirm that the DNA sequence is correct for any positives identified. The easiest and...
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Polysome Profiles: What You Need to Know

Has your supervisor asked you to do a polysome profile? Are you attempting them but running in to problems? This article will help explain what they are, the basics of the protocol and provide some helpful tips...
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Antibiotics Used in Molecular Biology

Antibiotics are used in a wide range of techniques in molecular biology. My aim with this post is to provide an easy reference to some of the main ones used in molecular biology, their mechanisms, range and...
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E.coli Electroporation vs Chemical Transformation

This is the first in a three part series on the transformation of E.coli. By the end of this you should be an expert on E.coli transformation and on which strains to choose for different applications. If you're...
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A Guide to Solid Phase Reversible Immobilization

  Scientists today depend heavily on many molecular biology techniques to perform their research. For example, with the advent of next generation sequencing (NGS): scientists are able to look at very...
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