This laminate-ready bench card gives you a six-factor decision table covering target count, RNA quantity, throughput, inhibitor risk, priming strategy, and enzyme choice. When a run goes wrong, use the troubleshooting flowchart to work through no amplification, inconsistent Ct, multiple melt peaks, or low yield.

Journal club is an opportunity to present cutting-edge research and take inspiration from your colleagues. If you’re wondering how to turn that impressive paper into stunning talk or simply don’t know where to start when organizing it all—relax. With our checklist, you can’t go wrong.