Scientific Illustrations Part I: Schematics and Cartoons

Scientific Illustrations Part I: Schematics and Cartoons

Biologists have a long tradition of drawing specimens as a form of data collection before the invention of the camera. The ability to present information in the form of illustrations is an important but often understated skill in a scientist’s toolkit. Scientific illustrations in publications run the gamut from schematics, 3D models, cartoons, and even…

A Simple Method for Measuring Intracellular Fluorescence

A Simple Method for Measuring Intracellular Fluorescence

Fortunately for microscopy users, measuring intracellular fluorescence has been made relatively simple through an ImageJ plugin called the Cell Magic Wand. For those of you unfamiliar with ImageJ, it’s a popular image processing program that runs on Mac, Windows, and Linux. How to use ImageJ for measuring intracellular fluorescence First of all, to begin measuring…

Get Your Single Nucleotide Polymorphisms Straight From the Oven!

Get Your Single Nucleotide Polymorphisms Straight From the Oven!

While it is true that there are some useful websites like SNPedia, or NCBI that can help you find rs codes for genetic variants, sometimes you need that info coming straight from the oven – particularly when you want to look at atypic SNPs or substitutions that have not been validated. So, in this post I…

How to Start Using Coding to Automate Image Analysis Part 1: The Simple Process

Pat yourself on the back, you saw a post with the word “coding” in the title and you didn’t freak out or glaze over. That’s the first step. Coding seems to have such a stigma attached to it; people tend to think that it’s incomprehensible nonsense that they could never learn, and that it has…

How to Start Using Coding to Automate Image Analysis Part 2: Batch Processing Multiple Images

How to Start Using Coding to Automate Image Analysis Part 2: Batch Processing Multiple Images

In Part 1 of this article, I introduced you to using code for basic image manipulation in ImageJ and working with the command recorder to expand your coding vocabulary. I covered how to make a simple macro, how to edit it and then save it to be run again another time. If you skipped the…

Being “Accuri-te” Through Cytometry: A Guide to Accuri C6 Software

A new lab toy to make it big in the last 5–10 years is the Accuri C6 cytometer (now under the BD umbrella), a low-cost instrument in comparison to the big boys. Lightweight, with a small footprint and straightforward maintenance, it’s often the cytometer of choice. It may be suitable for those labs that require…

A Guide to Flow Cytometry Software: Becton Dickinson’s ‘Diva’

A Guide to Flow Cytometry Software: Becton Dickinson’s ‘Diva’

If you use a Becton Dickinson (BD) cytometer in your lab, the chances are you are acquiring your data using ‘Diva’ software. Diva software is used to acquire your cytometry data on LSRII, LSRFortessa, CantoII and Aria cell sorters. As well as acquiring your data using Diva software, you can also analyse your data after…