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You know the drill. To prove your theory, you must show the colocalization of X and Y in a cell. Here are 2 ways to reveal protein colocalization.
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.
Proteins in the cell are in a constant flux governed by events including synthesis and degradation. In an effort to make cells more efficient by reducing the unnecessary protein load, most proteins in the cell have a specifically defined half-life. Another reason why cells have evolved to degrade proteins is to ensure timely removal of…
As any good biologist knows, one of the easiest ways to determine if a cell is functionally active is the production and secretion of proteins in response to a stimulus. In many circumstances, the quantity of the secreted protein, and thus the level of cellular activation can be assessed by ELISA. However, if you are…
When I was being trained in microbiology as an undergrad, one of the first skills I acquired was the ability to quickly compare and visualize amino acid sequences using BLAST and ClustalW. 15 years later, those two programs have done nothing but improve by expanding the data contained in these databases and simplifying the user…
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