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Tips for Successful Bone Marrow Isolation
…differentiation media and on certain cell culture plates. Enrichment media contain cytokines (i.e. M-CSF) that stimulate the growth of specific cells. Using specific cell culture plates (tissue-culture treated vs. non-tissue-culture…
Read MoreHow Do Buffers Work?
…media that can support the growth of a lot of cells. The cells will secrete various chemicals as they grow, live, and die. This is just part of their metabolism…
Read MoreCrash Course in Microbial Identification
…species. These tests are probably more reliable in combination with catalase/oxidase testing and microscopic examination. These tests overlap with the concept of selective growth media. A wide range of selective media exists…
Read MorePreparing Metaphase Spreads: The Breakdown on Broken-Down Cells
…harvesting! This almost works the same way as harvesting cell pellets, except you’ll retain the media with the colcemid, rather than pouring it away. Pour the colcemid-spiked media into a…
Read MoreKeeping Your Science Out of the (Junk) Headlines
…it is no doubt frustrating to see good science gone bad (or just bad science) perpetuated by the mainstream media catering to those with an interest in science at its…
Read MoreUsing Gage R&R Studies to Measure Precision in the Lab
…scientists are interested in supplementing media with an additive to determine whether it will significantly improve growth rates for your favorite cell line. To determine if the additive works, you…
Read MoreTissue Embedding and Sectioning: Something to Think About Whilst in the Bath.
In the same way that you should ‘Think Before You Fix’, the choice of embedding media should be dictated by your required end-point. The basic principle is that by processing…
Read MoreHomemade PCR Test for Mycoplasma Contamination
…when working with different cell lines, and aliquoting media) it is quite easy to test for mycoplasma contamination. While commercial test kits are quite easy and fast, they are often…
Read MoreIs Your Bacterial Culture Still Growing? A Primer on OD600 Measurements
…media pH as a result of cell metabolism, or an accumulation of toxic cellular waste. Figure 1. Phases of a bacterial growth curve. Using OD600 Measurements to Determine Your Bacterial Culture…
Read MoreAvoid These Pitfalls: Seven (Not So Deadly) Histology Sins
…not #1.5 coverslips are the most common. This is because the thickness of the mounting media must also be taken into account when calculating optimal ‘coverslip thickness’. Therefore a #1…
Read MoreHow To Fix Suspension Cells For Microscopy And Imaging
…which grow suspended in media and not on any surface – cannot be attached to your cover slips the same way. Read below to learn how to fix and ‘smear’…
Read More“Where the hell did my signal go?” AKA The Problems (and Uses) of ‘Photobleaching’ in Microscopy and Imaging
…light exposure and intensity you can use anti-photobleaching mounting media on your sample. These media (such as Vectashield by Vector, or Prolong by Invitrogen) can help slow fading and photobleaching…
Read MoreThe Basics of NMR Part 3: Multidimensional NMR
…coli (10s of milligrams per prep), the isotope labeling process is simple. Grow your bugs in M9 Minimal Media and switch out NH4Cl with 15NH4Cl and glucose with 13C-labeled glucose….
Read MoreMicroglial Cell Lines: The Ultimate Guide to Selection
…EOC 2 Mouse Spontaneous DMEM + 10% FBS + 20% LADMAC conditioned media BSL-1 ATCC CRL-2467 EOC 13.31 Mouse Spontaneous DMEM + 10% FBS + 20% LADMAC conditioned media BSL-1…
Read MoreDIY Electrocompetent E. coli
…mM \beta-mercaptoethanol to growth media and 0.03 mM in transformation media prior to electroporation has been shown to increase the transformation efficiency of competent cells. (5) Use a recovery medium—the…
Read MoreFinding a Needle in a Needlestack Using Phage Display
…it up. But, instead of seeing a bottle full of beautiful foggy amber, your media is as clear as when you inoculated it! And except for the look of wet…
Read MoreChoose the Statistical Package that Will Make Your Data Talk
…to do some quick hypothesis testing or determine the dispersion of the data. IBM SPSS Statistics Its name originates from Statistical Package for Social Sciences, which was the field it…
Read More10 Fun Hobbies for Scientists
…Being more supple will improve your overall lab fitness, protect you from poor posture and repetitive strain injuries, and make you feel better. It can be a social activity that…
Read MoreThe Easier Way to Write a PhD Thesis
…have to worry about losing your data. 12. Get Your Head Out of the Books Regularly Take time to think about your work, get a social life, and exercise daily….
Read MoreUseful Tips to Keep on Top of New Literature
…that you don’t miss any new content on a particular site, especially if you’re not a social media user. Definitely a tool to keep in your toolbox to help you…
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