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Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
IMS is a rapidly advancing technique that nicely blends the best bits of mass spectrometry with microscopic imaging. Learn more about it and how it could be applied to your work.
Predicting how proteins will fold in vivo is a Holy Grail of proteomics and theoretical chemistry. Current hopes are that this can be achieved by designing an in silico platform that can predict protein folding, either de novo (a.k.a. from scratch) or using known proteins as a guide. What would we need to do, why…
While most of us have heard of super resolution microscopy, many of you may not have heard of MSIM, or Multifocal Structured Illumination Microscopy. This under-the-radar imaging technique is relatively quick, cheap (by comparison) and will allow you to get a lot of data, fast. So What is MSIM Anyway? MSIM, as I mentioned earlier,…
In the frenzy of today’s scientific publication landscape, it is essential to take a proactive stand at your online visibility and reputation. There are online tools that can help you make yourself spotted and translate your hard work and high-quality science into actual impact. So if you are interested in improving your chance of landing…
Western blotting uses electrophoresis and antibody-epitope affinity to give a semi-quantitative and (theoretically) clear measure of protein abundance. It’s a long procedure, filled with many steps—and even more room for error. Learning to troubleshoot certain problems is incredibly important for continued success with this technique. So what do you do when your final imaged product…
After countless immunos with free-floating sections – troubleshooting, testing antibodies, and finally doing the actual experiments – I felt like an expert on immunohistochemistry. I knew everything there is to know, right? Well, of course not – it does not work like this in science! For my next project, I would need to perform immunohistochemistry…
Read our top tips on how to write a scientific review, from gathering your information to getting it published.
The human brain autofluoresces—a funny thought next time you see a cartoon character with a bright idea and a light bulb over his head—but not so funny if you are attempting immunofluorescence analysis. But there are some significant advantages to using fluorescence detection over chromogenic methods. In this article, I will cover the advantages of…
If you find it very difficult to call your boss by her first name, feel obligated to wear business casual clothes to the lab, or reckon that it’s slightly weird that your fellow PhD candidates are inviting you to a casual night out instead of to the library to study, then like me, you just…
Some tissues are tricky to work with. This truth was lost on me in the early years of grad school because I worked with liver samples. If you’re extracting RNA from liver samples, you’re likely not losing sleep over your massive RNA yields. But for the folks doing RNA extractions with less willing donors, such…
We’re already gone through the basics of how gel electrophoresis work, compared common gel types like agarose and polyacrylamide and even explored some alternatives. Now let’s look at the native versus denaturing gels. You’ll be a speGEList in no time! Denaturing Gels We’ll start with this one, as it’s very self-explanatory. Denaturing gels are exactly…
In real life, cells are instructed to commit suicide for the greater good of the organism. The programmed cell death (apoptosis) is important during development of a multi-cellular organism. A good example you will appreciate is the dis-appreance of the tail from a tadpole as it turns into a frog. On the reverse, the lack…
This article mentions of suicidal thoughts. If you are having thoughts of self-harm, I encourage finding someone to talk to. It can be a family member, friend, or professional counselor. Many countries also have suicide hotlines. Mental Health is often not a priority for institutions or individuals in academia. Making institutions friendlier will take time,…
Please note that SNPper appears to be a discontinued legacy resource rather than a currently usable database. While it is true that there are some useful websites like SNPedia, or NCBI that can help you find rs codes for genetic variants, sometimes you need that info coming straight from the oven – particularly when you want…
When you first start out using a microscope, you might only adjust the eye pieces, objectives, and the focus controls. However, you shouldn’t overlook the microscope condensers as they are an important part of the whole optical system of a microscope.
Normally you need two primers to amplify your segment of interest – one for the 3′ end of your segment of interest and one for your 5′ end. But if you don’t know the sequence of the regions you’re hoping to amplify this can be a problem! Rapid Amplification of cDNA Ends (RACE) is a…
Lab work, as we are all aware, comes with many pressures: one of which is productivity. You want to generate as much quality data as possible to meet publication deadlines or perhaps the elusive thesis. Sometimes it may feel like hours spent in the lab don’t match the amount of data produced: for some this…
So, you’ve extracted your precious RNA and want to check its quality on a gel. Conventionally, you would run a formaldehyde gel, which is messy and requires a lot of prep. Plus, it is a huge undertaking in terms of time (and money) if all you want to do is just check the quality of…
The ability for DNA polymerase to copy a long stretch of DNA is becoming increasingly important. Why? It has to do with the advances in our sequencing technologies. Our next generation sequencing (NGS) technology requires the DNA polymerase to copy a long stretch of DNA (sometimes up to 50kb) as NGS is churning out genetic…
Choosing a PhD topic can be very hard. There are a lot of things to consider from the subject to the supervisor. Here are some tips to help you choose. Find out what you really like This is the first topic because it is the most important. My first advice would be to get some…
Anyone working with laboratory animals has probably realized that simply putting two animals together does not always yield new offspring and reliable continuity of the animal line – unfortunately animal husbandry isn’t that simple! Of course, apart from making sure that the two animals put together are from different genders, there are a lot of…
Before I get into today’s topic, please allow me to digress a bit and start with a few sentences that sum up the polymerase chain reaction (PCR); the grand-daddy of molecular biology. PCR, a method that is at the heart of modern day molecular biology discoveries, is a process that amplifies genetic material through our…
Let’s face it, at least once in your lab life you are going to need a favor. You may need to go on vacation, you may be sick or you may just be a little overwhelmed at the bench. At least once, you will need to ask for a little help and someone will surely…
We can, however, use special dyes or engineered proteins to bind to calcium, indicating where in the cell it is. The trick is determining which probe is most suitable to your cells and application.
Having just finished graduate school, I have been given the privilege of nearly unlimited time to reflect (Yay! Unpaid, Boo!). Graduate school was, for me, a juxtaposition of intellectual growth, real-world learning and great fun. An introduction to adulthood with training wheels—while simultaneously being a blur of anxiety, work, sleepless nights and existential crises. I…
As a dual-degree MD/PhD student I spent two years in the medical school doing classroom learning, now I’m in the lab trying to get a PhD, and then in a few years I’ll return for the last two years of medical school and work in the hospital. In this process, I’ve heard a lot about…
After ten years of postdoctoral research there is one important piece of advice I would give to anyone embarking on a research career: Spend as much time managing your data as you do generating it Take time at the beginning of each project to organize how you will record what you are doing day-to-day. The…
PhDs have been known for their nightmarish effects on students’ psychological wellbeing, to the point that the acronym PhD has also been dubbed ‘Permanent Head Damage’, ‘Philosophically Disturbed’ or ‘Please Help. Desperate’. Doing a PhD is an emotionally exhausting experience rather than being physically challenging. Here are some tips on how to survive the PhD…
As a protein biochemist where bacteria were mere workhorses, imagine my surprise when I began work in a bonafide micro lab! I discovered that bacteria could be much fussier than my good ol’ cloning and expression friends E. coli DH5ɑ or BL21. One broth would not do for all, some even required blood! No, no,…
Few things can dash your hopes quite like phages. They can annihilate whole bacteria cultures in the blink of an eye, and make your next cloning or expression project impossible. But you can harness these evil-do-ers for good. And use phages to screen massive libraries of peptides. Learn how below. The Typical “Evil” Phage Experience…
Flow cytometry is a fluorescence-based technology, as is fluorescence microscopy and confocal microscopy. Fluorescence is fundamental to how a cytometer gathers data, but I am often surprised, as a core manager, at how little new users know about the process of fluorescence. So, this is where I always start the training process. Let’s get physical…
A busy research lab can be a challenging place to work for a variety of reasons. Science is a high pressure environment, often with looming grant or research paper deadlines, troublesome reagents, and experimental failure. Combined, these factors can cause many kinds of strife in the workplace, here are a few examples and some of my top tips for dealing with them!
If, like many people, you use fluorescent proteins to view your transfection efficiency or your CRISPR gene editing, you can also isolate cells with different expression levels of fluorescent proteins. Here are 5 ways to improve your sorting experiment.
Most eukaryotic proteins exist as several isoforms, differing in posttranslational modifications, which allows them to perform slightly different functions or the same function under slightly different conditions. A common posttranslational modification of proteins is glycosylation.
When it comes to registering the signal output of your Southern/Northern/Western/probe hybridization, you are spoilt for choice these days. You can go all retro and use X-ray film. You can go digital and use a phosphorimager. Finally, you can go fluorescent and use a fluorescence detector. So, what are the pros and cons of each…
Recently, I have witnessed the uprising of various next generation sequencing (NGS) platforms and it’s quite interesting because each platform uses a different method. Previously, I’ve written about the exciting possibility of nanopore sequencing—a new sequencing technology based on the “signature” electrical currents generated as a single strand of DNA passes through the nanopore. The…
So it’s time to write up your thesis. By now, you probably consider yourself an expert on your topic or you hate the sight of it (or both!). Either way, hundreds of typewritten pages are all that stands between you and graduation/freedom. One of your first requirements will be to review the current knowledge on…
Mammalian cell culture techniques are not simple, and culturing the cells requires a lot of maintenance as well as patience. In addition, doubling times compared to bacterial cells can take days instead of hours, which is most evident when contamination occurs. However, implementing small-scale hollow fiber bioreactors for culturing mammalian cells can save a lot…
When restrictions come in the form of paperwork and approvals, we detest them. Whereas, when the restrictions come in the form of enzymes, we love them, don’t we? Restriction enzymes play a key role in biotechnology research. Read ahead for six useful facts about restriction enzymes. 1. Restriction enzymes are helpful to bacteria Restriction enzymes…
Want to save money on one of the most expensive steps of your Western blots? Then, use less antibody for significant cost savings!
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