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Basic Local Alignment Search Tool (BLAST) remains the cornerstone of biological sequence analysis. When users have one or few queries, the data are easy to interpret based on the graphical output that the search provides. However, when you have to analyze hundreds or a few thousand queries, running graphical outputs can be computationally intensive, difficult…
In part one, I discussed the ‘how to’ of simply freezing samples and the basics of vacuum evaporation, often referred to as speed vacing. Now, we’ll have a look at two more complex sample storage techniques (at least in terms of equipment) for drying samples (lyophilizaton and rotary evaporation) and the simpler method of blow…
Graduate students in the United States are privileged when it comes to picking their prospective labs: most programs have student rotate through several laboratories to help them choose their PhD lab. Here are some suggestions for questions to ask.
Pat yourself on the back, you saw a post with the word “coding” in the title and you didn’t freak out or glaze over. That’s the first step. Coding seems to have such a stigma attached to it; people tend to think that it’s incomprehensible nonsense that they could never learn, and that it has…
In the frenzy of today’s scientific publication landscape, it is essential to take a proactive stand at your online visibility and reputation. There are online tools that can help you make yourself spotted and translate your hard work and high-quality science into actual impact. So if you are interested in improving your chance of landing…
In Part 1 of this article, I introduced you to using code for basic image manipulation in ImageJ and working with the command recorder to expand your coding vocabulary. I covered how to make a simple macro, how to edit it and then save it to be run again another time. If you skipped the…
A number of studies have shown that work–life balance, as well as personal happiness, positively correlates with productivity, professional achievement and success. Looks like that happiness is not only a luxury but it might be a key ingredient for success.
Choosing a PhD topic can be very hard. There are a lot of things to consider from the subject to the supervisor. Here are some tips to help you choose. Find out what you really like This is the first topic because it is the most important. My first advice would be to get some…
Finishing my third year of grad school, I can’t help but notice what a balancing act it has been- research and relaxation, finances and fun, beer and…not beer? I wanted to write this article to advise incoming first years and veteran grad students – even those mysterious 7th year students- on graduate school and the…
Interested in whether your protein uses oxygen to mediate reactions? Wondering if oxygen is keeping your enzyme from its duty? Then what you need as an anaerobic tent! These tips provide some basic knowledge to help you perform experiments using an anaerobic tent. What is an anaerobic tent? Most biologists who work in oxygen-free environments…
A PhD, like any worthy endeavor in life, requires hard work, patience and perseverance. It also involves challenges that will make you doubt your choice to pursuit an advanced degree in the first place. It is inevitable that a series of failed experiments or that one paper rejection will bring you down and temporarily make…
Rust spots provide a good shelter for bugs, which will get there one day, and from the rust into your tissue culture. Here’s what you can do to deal with the problem as soon as you see it.
At a conference, a poster can be a great tool for drawing people into conversation. Not only do you have something to break the ice with, you can also get really useful feedback from a completely different perspective! To make sure you get the most out of your poster time it’s important to have a…
As a scientist myself, I have always been focused on performing experiments to achieve my sole goal of producing data for a publication. Recently I started paying attention to the waste I solely generate on a regular basis in the lab. Starting from disposable Eppendorf tubes and pipette tips, to labels and Petri plates, it…
Whether you’re just beginning, or nearing the end of your undergraduate career, getting involved in research can be overwhelming and stressful. There are many factors to consider, so keep it simple and focus on the most important ones: What area of research motivates you? What organisms do you find interesting? Are there any research professors…
Sometimes, you have to work with radioactive materials in the lab. If you happen to be one of those lucky individuals, it is important to take extra precautions in your work to ensure your safety, and good results! Here are my tips to make sure you radiate confidence doing your experiments. Know Your Element It…
Innovation is the essence of research and it is the fuel that keeps the research industry going. If it were not for inventions, the field of scientific research would have dropped dead long ago. However, simply innovating something is not sufficient. It is not the end of the road, but the beginning of it. Therefore,…
Let’s face it, at least once in your lab life you are going to need a favor. You may need to go on vacation, you may be sick or you may just be a little overwhelmed at the bench. At least once, you will need to ask for a little help and someone will surely…
Here are some ideas to make your liquid handling robot perform at its best.
Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other international scientists. The problem is that I don’t know how to address the situation. If they indeed have antibodies that…
You can’t put it off any longer – you need to write your first manuscript. This task can seem daunting at first, but with a little organization and planning, you’ll soon be publishing.
Have you read an article How To Start Your PhD The Right Way? If you have, then you may have already decided that you are going to do a PhD. The next step is to choose your supervisor – this is a very important decision to make. When I chose my supervisor, I had only…
Working in a lab can be stressful, there is so much to do, and so little time to do it that we often feel overwhelmed. Sometimes you come into the lab and there are 15 experiments that just need to be done, so you throw on some music and start doing a bunch of them…
Need tips for surviving being the newbie of the lab, and what you can do to handle all the possible personality types you may encounter. Read on.
So it’s time to write up your thesis. By now, you probably consider yourself an expert on your topic or you hate the sight of it (or both!). Either way, hundreds of typewritten pages are all that stands between you and graduation/freedom. One of your first requirements will be to review the current knowledge on…
In today’s times, fierce competition exists in the field of science and technology for positions like Ph.D. or research assistantships. Hence, gaining such a coveted place in a new lab is indeed a moment of exultation for any student. However, it is extremely important that before we embark upon this new journey, we make some…
If you find it very difficult to call your boss by her first name, feel obligated to wear business casual clothes to the lab, or reckon that it’s slightly weird that your fellow PhD candidates are inviting you to a casual night out instead of to the library to study, then like me, you just…
How do we decide whether our research is having an impact or not? Is there a way to quantify the effect or judge the quality of the articles? Read on to find out about the nuances behind “impact factor.”
A busy research lab can be a challenging place to work for a variety of reasons. Science is a high pressure environment, often with looming grant or research paper deadlines, troublesome reagents, and experimental failure. Combined, these factors can cause many kinds of strife in the workplace, here are a few examples and some of my top tips for dealing with them!
Read our top tips on how to write a scientific review, from gathering your information to getting it published.
“It didn’t WORK!” The panic was tangible. Anyone who performs PCR on a regular basis is used to the ups and downs involved, but it occurred to me that this might be the first time in the student’s academic life where he had actually failed at something.
It is amazing to get that “Eureka!” moment or finally get that assay to work, but the downside of those moments is that we do face a lot of rejection. Scientists push the envelope of human knowledge and that means we have to deal with a fair amount of negativity in our line of work….
Having just finished graduate school, I have been given the privilege of nearly unlimited time to reflect (Yay! Unpaid, Boo!). Graduate school was, for me, a juxtaposition of intellectual growth, real-world learning and great fun. An introduction to adulthood with training wheels—while simultaneously being a blur of anxiety, work, sleepless nights and existential crises. I…
Stuck in a rut? Here are some useful nuggets you could implement in your day-to-day science life.
If I had a barrel of apples for each time I’ve heard one of my classmates or friends say, “Oh, I want to work in a lab, but I don’t know how to find one” I could build a moon base out of apples. Working as an undergraduate will help you land sweet internships, look…
There are many kinds of supervisors out there, ranging from the amazingly laid back to the crazy micromanagers. There are various strategies for dealing with all of them, but what do you do when your boss is a bully?
As a dual-degree MD/PhD student I spent two years in the medical school doing classroom learning, now I’m in the lab trying to get a PhD, and then in a few years I’ll return for the last two years of medical school and work in the hospital. In this process, I’ve heard a lot about…
Deciding to start a family with your partner is one of the most exhilarating and frightening times you’ll likely ever experience, outside of welcoming the little F1 to the world that is. Your job should not be an inhibitory factor in making that decision. We all know that post-docs live in a purgatory of sorts:…
It’s official. You’ve signed on the dotted line and you are about to begin the most exciting and frustrating journey into the depths of the unknown: a journey otherwise known as a PhD. You’ve heard the horror stories from previous students; the cloning that wouldn’t work for reasons unknown to man, the data that indicates…
Read our top tips to help you get the most out of your scientific literature surveys.
The eBook with top tips from our Researcher community.