Techniques / Genomics & Epigenetics

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Article

An Introduction to Shotgun Sequencing: Fire in the Hole

In the midst of all the cool new sequencing techniques and technologies out there today, you may have overlooked the tried and true method of Shotgun Sequencing. What is Shotgun Sequencing Anyway? Shotgun...
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Article

How to Choose The Appropriate Genomic NGS Data Simulator

Let’s say that you’ve just finished gathering your NGS reads and you’re going to simulate the introduction of random mutations at specific rates into the reads. Before you move on to the next step, you...
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Article

P19 to the Rescue: How to Increase Protein Expression in Agroinfiltration

Plants are just not green gods—they can be more. You can cost-effectively express your recombinant complex proteins in a plant system. More interestingly, plants are ideal systems for producing functional...
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Sponsored Education

DNA Sizing Tutorial: When to Use Manual Gels, Beads, and More

There are several methods for size-selecting DNA fragments prior to sequencing. How do you choose which is best? Here’s a look at various options, plus considerations to help you determine when to use each...
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Article

The Ins & Outs of Illumina Sequencing

The future of personalized medicine depends on affordable DNA sequencing. In the race for the $1,000 genome, several sequencer manufacturers are working on making equipment that can sequence DNA and RNA faster...
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Article

RNA-seq: The Challenges to Diving Right In

It’s the hot new technique. With a single procedure, you can get information about all RNA transcripts at once! It sounds like a dream. While RNA sequencing (RNA-seq) has opened the door to exciting new...
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Sponsored Education

For Better NGS Assemblies, Use More Precise DNA Size Selection

It may not be intuitive that a sample preparation step like DNA size selection would have a significant impact on downstream data analysis, but NGS users have proven that it does. Indeed, the precision of your...
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Article

Genome Viewers/Editors – Three of the Best

Sometimes the wide view is the best so a good genome viewer is a must for every molecular biologist. We review three leading genome viewer packages to get you started..
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Webinar

All about miRNAs: Practical Tips, Advice, and Applications

In this webinar you will learn the tips and tricks necessary for you to successfully work with miRNAs, and how you can use them to further your research. The main points we will cover are: Tips and...
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Article

Where Did It All Go Wrong?! Quality Control For Your NGS Data

You’ve carefully collected your samples, extracted nucleic acids and made your first set of next-generation sequencing libraries. How are you going to know if the data you get back is any good and whether it...
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