Bis-Tris Gels: Their Key Advantages and How to Prepare Them
Fat and blurry bands on your SDS-PAGE gel can leave your experimental question unanswered. Learn how you can sharpen up your bands and get much greater resolution by using Bis-Tris gels.
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Fat and blurry bands on your SDS-PAGE gel can leave your experimental question unanswered. Learn how you can sharpen up your bands and get much greater resolution by using Bis-Tris gels.
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Discover ways to perform total RNA extraction from TRIzol to kit-based and even old-school methods.
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Want to know not only if your proteins bind but quantify the interaction? We walk you through how to perform a quantitative yeast two-hybrid assay.
The use of viral delivery systems to transduce cells for gene and protein investigations has become prominent over the last 20 years. In particular, the use of lentiviral vectors permits stable expression of your gene of interest. This is all possible with a little bit of nucleic acid magic. Lentiviruses (a genus of retrovirus) express reverse…
During my first year as a graduate student, one of the earliest pieces of advice that I received from a senior student in the lab was to keep detailed protocols. In fact, she had a folder of her own protocols, all of them extremely detailed and riddled with notes. When she showed me how to…
Machine learning methods for challenging cells such as hepatocytes