Do you wonder if your favorite protein is entwined in a delicate dance with another protein? Do you wish that you could shine a spotlight on your protein to determine its dance partner? Well good news! While there is no such spotlight, you CAN use co-immunoprecipitation (Co-IP) to find your protein’s dance partner. You can then find out how they dance together by using surface plasmon resonance (SPR).
This article will get your ready for your first Co-IP and will touch upon how you can use Co-IP with SPR applications to gain a detailed insight into how your protein interacts with others.
The Basics of Co-IP
In essence, Co-IP is just a more delicate version of a traditional immunoprecipitation or IP. With both techniques, you use antigen/antibody interactions to isolate proteins. Your protein-of-interest is “pulled down” and out of solution by an antibody, which is in turn captured using beads.
However, a Co-IP requires greater care and more physiologically relevant conditions than traditional IP. When successful, Co-IP pulls down not only your protein-of-interest but also its interaction partners. Thus, Co-IPs are a great way to identify protein complexes. Furthermore, you can use Co-IPs to determine protein:protein interactions under varying conditions. You can then go on to study binding kinetics using SPR techniques.
The Nitty Gritty
Co-IP protocols are very similar to traditional IP protocols, with the difference that Co-IPs require more gentle assay conditions. The general steps are as follows:
1. Lyse your Cells
In this step you gently break open your cells to make your protein accessible to the antibody. The method of lysis is important in Co-IPs. Non-detergent, low-salt lysis buffers are a popular choice for Co-IP of soluble proteins. This kind of lysis is least likely to disturb any protein interactions. For less soluble protein complexes, however, lysis buffers may need to contain non-ionic detergents such as NP-40 or Triton X-100. Set time aside to test a number of lysis conditions to find the optimal lysis solution. Some things in science, like the best lysis buffer, just need to be determined empirically.
2. Add your Antibody
In this step, you add an antibody specific for your protein-of-interest. It can be difficult to choose the best antibody for a Co-IP but many antibody manufacturers provide helpful information in their product selection guides. If possible, choose an antibody that recognizes an epitope on your protein-of-interest that is not masked by any other protein:protein interactions.
3. Add the Protein A/G Beads
In general, beads are used to physically pull down and purify the antibody/protein complex from the rest of your mixture.
There are two main types of beads you can use: beads coated in protein A or beads coated in protein G. Protein A and G are specialized bacterial proteins that recognize and bind to antibodies. The specificity of your antibody will determine which of these two types of beads you should use. To determine which one is correct for you, consult your antibody’s manufacturer.
Traditionally, immunoprecipitation beads were agarose spheres (50-150uM in diameter) that allowed purification of the antibody/protein complexes via centrifugation. Centrifugation methods include the use of bead slurries or spin columns. Spin columns are generally fast and come as part of a kit with highly optimized protocols. However, they may be an expensive option. Bead slurries on the other hand, are great for larger complexes that require very gentle handling, without high centrifuge speeds.
More modern techniques use magnetic beads of 1-4uM in diameter instead of agarose beads. Magnetic beads allow you to isolate your antibody/protein complex by…You guessed it!…Magnets! Magnetic beads may be better for high-weight protein complexes and processing low volume samples to avoid excessive sample loss during centrifugation. Overall though, agarose beads often have a superior yield since they have a large surface area on which multiple A/G proteins are linked.
This is the time for the bead/antibody/protein interactions to occur. Incubation times vary from one hour to overnight depending on which bead you choose. Often, the incubation step includes gentle agitation, such as on a rocker plate at low speed. Agitation enhances the opportunity for interactions.
After incubation you will need to collect the antibody/protein complexes. Depending on the method you are using, you will either use magnets or centrifugation to separate the complexes from the rest of the cellular proteins.
6. Wash the Beads
Now that your proteins-of-interest are hopefully tethered to your bead via your antibody it is time to get rid of everything else in your lysate. Do this by washing your antibody/bead complex a few times to clear away cellular matter not specifically bound by your antibody. Washing also helps reduce non-specific binding of “sticky” cellular components to the beads. Generally, you wash using cold lysis buffer or cold PBS. You may need to optimize this step to find the right level of stringency that doesn’t disrupt the protein:protein interactions.
Expert Tip: Save all of your used wash buffer during this step. The wash buffers can show you if your protein-of-interest and any partner proteins were depleted from the lysate. This is critical knowledge for troubleshooting your Co-IP!
7. Elute your Protein(s)
Most SDS-PAGE loading buffers reduce and denature proteins. Therefore, it is often an effective way to separate the proteins from the beads. If you plan on using SDS-PAGE as your detection method (which most of us do), then you can add loading directly to the beads to harvest your protein(s).
If you want to detect your proteins using native PAGE protocol, or you plan on doing enzyme assays with your isolated proteins, then you may need to use something other than SDS-PAGE loading buffer to elute. A frequently used non-denaturing elution buffer is 0.1M glycine at a low pH (around 2.5-3). However, some proteins will still denature or loose enzymatic activity under these conditions, and some proteins may not dissociate with this treatment. So chalk this up to yet another step to experiment with.
8. Detect your Protein(s)
By now you have isolated your protein(s) and are ready to go. (Yay!) Now the exciting part…taking a peek at your isolated protein-of-interest and its dance partner(s). How you want to do this – SDS-PAGE, mass spec, enzymatic assays, etc. – is up to you.
Expert tip: Remember that the antibody you used to purify your protein is also in the eluent along with your protein-of-interest. And this can be a problem. A western blot may detect the antibodies in your eluent, thus masking the detection of your proteins-of-interest if they are close in molecular weight. (The molecular weight of an antibody’s heavy and light chain are about 50 and 25 kD, respectively). You might be able to overcome this issue by 1) using beads covalently bound to the antibody, or 2) by ensuring that the secondary antibody chosen for your western blot recognizes a different species than that of your Co-IP antibody.
9. Study the Interaction With Surface Plasmon Resonance Applications
A positive finding in a Co-IP is great news, but you should temper your excitement. Many scientists are led astray by sticky proteins. You should do additional studies to confirm any potential interactions and to ensure that they are specific. For example, you can use mutational analysis to map binding sites. Alternatively, for more specific information about an interaction, consider using an SPR application.
SPR applications not only confirm interactions between binding partners, but they also provide quantitative measurements of the affinity, thermodynamics and kinetics of these interactions in real time. A SPR biosensor that allows measurement of the interaction at different temperatures facilitates a thermodynamic analysis of the interaction of interest. With SPR, you can confidently compare affinities between your protein of interest and different binding partners. A major practical advantage of SPR is that you don’t need to label your protein of interest (so no more tag-cloning or radioactivity), saving you time and potential hassle with tricky cloning strategies.
If you want to detect and unravel interactions between immune receptors and their ligands, you might want to consider trying a biosensor assay. As explained in a previous Bitesize Bio article, biosensor assays allow you to measure the rate of association and dissociation of ligands to their specific antigen receptors. These assays follow SPR principles. Due to their advantages and vast capabilities, SPR and its related applications have become the gold standard for kinetic and affinity determination, and SPR principles underlines most color-based biosensor chip applications.
More Expert Tips
Of course, within these steps there are many variables that can make your Co-IP successful, or leave you with a sad face at the end. And there are a myriad of companies, products and kits from which you can choose. So prepare yourself for some trial and error!
Before we finish up, here are a few more expert tips to delicately sneak a peek at your dancing proteins without disturbing them too much.
Clear the Dance Floor First
“Pre-clear” your lysate by adding protein A/G beads to the lysate before introducing your antibody. Next, spin down the beads and discard them to pull out any cellular components that stick to the beads unspecifically. Then add your antibody/beads and proceed. Pre-clearing can help to reduce non-specific binding and reduce potential background. However, if you plan to use western blotting to detect your proteins at the end of your Co-IP, pre-clearing is probably not necessary unless you know that a contaminating protein is likely to interfere with your protein/band of interest.
Plan the Order of Dance Steps
Introduction of the bead to the antibody is an important variable that can affect yield. Typically, the antibody is immobilized to the bead by incubating them together before introducing the lysate. However, if the protein-of-interest is present in low concentrations, or if the antibody has a weak affinity for your protein-of-interest, you may need to incubate the protein and antibody first, then add the beads.
Go Gentle, Go Slow
Your goal is to maintain the protein:protein interactions. Your antibody/protein complexes will be physically pulled out of solution by protein A or G conjugated beads. And while the tether from bead to antibody is highly specific, it is tenuous. Therefore, treat your cell lysate with velvet gloves. This includes choosing the right lysis method, keeping your samples chilled and avoiding over handling. Vortexing, mixing with small pipette tips, or using high spins in the centrifuge can all break apart your delicate chain of protein/antibody/bead, and fling your proteins back into solution.
So, are you ready to find your favorite protein’s dance partner?
Originally published in 2014. Updated and republished in May 2017.