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…done by transferring pieces into a centrifuge tube containing around 0.5 mL of incomplete medium. Gently mix by pipetting the medium 4 to 5 times, and allow the pieces to…
…material in them to create a barrier. This barrier prevents what you are pipetting from aerosolizing and contaminating your pipette. Although barrier pipette tips are pricier than your normal run-of-the-mill…
…that reduced concentration and our ability to do the job. “Rubbish!”, I thought, “Nothing helps you through a mindless task like splitting cells, pipetting or labelling like a bit of…
…pipetting errors, inefficient cDNA synthesis and inaccurate quantification will lead to a quantifiable difference. Thus, a good housekeeping gene provides a point of reference to normalize all values to and…
…and there through pipetting, so make sure to take that into account. Oligo Resuspension Calculator This is a helpful little tool to calculate the exact volume of water or TE…
…beautiful pipettes have been ruined by sheer incompetence… 2. The wrong guy for the job Make sure your tip is the right one for the volume you are pipetting. You…
…trying to do it all at once and making a mess of the experiment. Grand research ambitions and confidence can be scuppered by the first pipetting mistake at the bench…
…is simply an effect of poor mixing of the sample. Before giving up on the sample or moving it to a new concentrator, try gently pipetting the remaining solution over…
…resources (like reference guides for qPCR). Pipetting technique is really important to maintain accuracy. If training a student completely new to the lab, then getting them to practice will save…
…you will see a more viscous protein solution close to the membrane swirl up and diffuse into the less concentrated solution. When pipetting, take special care not to touch and…
…liquid exchange is done by pipetting, so it is easy to accidentally lose sample in the washes Diffusion is slow, so long incubation steps are required Long incubation and extensive…
…the innocent colleague who thinks it’s rude not to react, see if you can politely ask to have all your attention to pipetting. If experiments lead you to eat lunch…
…from mechanical shearing due to pipetting. However, it is important to note that DNA prepared with silica columns is typically in the range of 20-50 kb and this is large…
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