Following closely on the heels of Cristy’s article “How to Clean a Waterbath”, I’d like to take a moment to rant about a few other hated (and carefully avoided) lab tasks. Here are my top ten LEAST favorite things to do in the lab:
Cleaning out the vacuum trap – truly gag-worthy…you never know what your colleagues have been sucking up in there.
Defrosting the -20°C freezer – it’s slow, it’s messy, and you’ll just have to do it all over again in a month or two.
Defrosting the -80°C freezer – even worse, due to the possibility of accidentally freezing body parts to the inside of the freezer, or losing a finger to frostbite.
Filling the liquid nitrogen tank – I always feel like I’m taking my life in my hands when I do this! Something that is so dangerous shouldn’t slosh so much, you know? To help you feel more comfortable handling liquid nitrogen, you should make sure you that you get appropriate liquid nitrogen safety training.
Updating the chemical inventory – this would be so much easier if you just updated it every time you ordered a chemical, but when has that ever happened?
Annotating plasmid maps – carefully piecing together the sequence for the clone you just made sometimes seems like it takes even longer than making the clone itself!
Making competent cells – the centrifugations, the aliquoting, stressing out over sterile technique…it doesn’t get any better than this!
Aliquoting – competent cells, antibodies, dNTPs…just about anything, really.
Making up 10M HCl or NaOH stocks for adjusting the pH of solutions – scary scary scary.
Racking pipette tips – have you ever been in a lab cheap enough to buy loose pipette tips that need to be individually inserted into the tip boxes before autoclaving? A sure-fire way to drive your summer student crazy.
Most of us hate cleaning and are often hard pressed to find time to clean our homes, never mind our laboratory space. However, an annual spring clean and maintenance of a regular cleaning rota/regime will contribute to an efficient, organized and harmonious lab environment. This is increasingly important in communal lab spaces where multiple staff…
I write regularly about topics related to electronic lab notebooks over on The electronic lab notebook blog. Nick thought it might be interesting for me to do an occasional post on electronic lab notebooks for Bitesize Bio, so here goes! I thought I’d start with a comparison of electronic lab notebooks with wikis and Google…
Have you got a plasmid that others are begging to use? Perhaps your cell line is in high demand? We’ll show how using a repository can take the hassle out of sharing your reagents.
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