Antibiotics Used in Molecular Biology

October 2, 2007 | By Nick In Tech Tips |


antibiotics.jpgAntibiotics are used in a wide range of techniques in molecular biology. My aim with this post is to provide an easy reference to some of the main antibiotics used in molecular biology, their mechanisms, range and working concentrations. I hope you will find it useful. If I have missed out an antibiotic that you use routinely in your work, please feel free to contact me or leave a comment and and I will add it to the table.

Ampicillin- Beta-lactam-transpepsidase-Amphotericin B-cholesterol-Carbenicillin-peptidyl transferase-translation-Chloramphenicol-acetyltransferase--Erythromycin-Macrolide-ermC methyltransferase-Kanamycin-Aminoglycoside-initiation complex frame-shift-Kanamycin phosphotransferase-Gentamycin-Nystatin-Inhibits DNA dependent RNA polymerase-Streptomycin-Prevents aminoacyl tRNA from binding to 30S sub-unit-tetracyline-TetR-TN10

*Abbreviations: Gm(+/-)=Gram positive/negative; My=mycoplasma; F=fungus; Y=yeast. Ampicillin

Photo: Hugovk


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3 Comments so far

  1. Biology In The Blogs: MoBio || Molecular Biology.. » Science Discoveries . net on October 2nd, 2007

    [...] Antibiotics Used in Molecular Biology Antibiotics are used in a wide range of techniques in molecular biology. My aim with this post is to provide an easy reference to some of the main antibiotics used in molecular biology, their mechanisms, range and working concentrations … [...]

  2. The Best of: Tech Tips | The Molecular Biology Blog on November 22nd, 2007

    [...] Antibiotics Used in Molecular Biology [...]

  3. Ariel on July 24th, 2008

    In my lab we generally use 7 ug/mL for gentamycin

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