Antibiotics Used in Molecular Biology |
Antibiotics are used in a wide range of techniques in molecular biology. My aim with this post is to provide an easy reference to some of the main antibiotics used in molecular biology, their mechanisms, range and working concentrations. I hope you will find it useful. If I have missed out an antibiotic that you use routinely in your work, please feel free to contact me or leave a comment and and I will add it to the table.

*Abbreviations: Gm(+/-)=Gram positive/negative; My=mycoplasma; F=fungus; Y=yeast. Ampicillin
Photo: Hugovk
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Ariel
In my lab we generally use 7 ug/mL for gentamycin
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sallap
Thanks for the table!
Hygromycin B can be added.
and we’ve been using Kan at 50ug/ml without problems.