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last updated: February 4, 2022
Jason has a BS in biochemistry, runs experiments in a behavioral neuroscience laboratory at Oregon Health & Science University, and creates custom tools to increase the speed and efficiency at which the building blocks of your scientific discoveries can be made.
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The study of geometric probability in stereology may seem like a large, overwhelming area of focus but, don’t panic, it’s not! In fact, it is a very specific field that is likely to come easy to those that excel in mathematics or science (yes that means you). However, the concept is not one that most…
We have to rely on artificial systems to test our hypotheses and often have to come up with original set-ups to investigate specific problems. One of these creative inventions is the use of supported lipid bilayers.
Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for Biologists)(Fig. 1). While the resultant images appear to be three dimensional (3D), they actually contain no depth information. However, there are several SEM techniques that can obtain…
So far in this series I have given you an overview of how STED works and how to design your STED experiments. In this final article, I will tell you how to do two color STED and go over some tips and tricks to acquire the best images from your sample. Dual Color STED Fluorophore…
If you’re thinking FRAP is short for frappuccino then you need to read this article. Discover the history, how it works, and why you’d want it in your confocal toolbox
You’ll give me an (enzymatic) complex! Following on from Part 1 of this article, let’s start by having a look at the two most popular enzymatic ‘sandwich’ methods; The Peroxidase anti Peroxidase method (PAP). The PAP method was the first sandwich method that I used and involves three main stages- application of primary antibody, secondary…
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