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Need help choosing a luciferase assay? This guide walks you through the differences between Firefly, Renilla, and NanoLuc reporters, plus key tradeoffs in signal duration, brightness, and workflow compatibility. Learn how to match the correct assay to your experimental needs.
Discover a better way to improve the expression of your insoluble and inactive proteins using osmotic shock.
Explore the pros and cons of key DNA Cloning techniques such as TA, Golden Gate, and Gateway cloning, and find out how to manage DNA complexities in our guide to Molecular Cloning.
Follow our simple lentiviral transduction protocol and discover expert tips and tricks to make your transductions a success.
Discover what unnatural amino acids are, their applications, and how they can be used in your research in our beginner’s guide.
Want to reduce the use of antibiotics in the lab? Start with switching to alternative cloning methods that use alternative selection pressures.
So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first. Now, it’s time to put your expression plasmid into E. coli and get…
Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will reduce my transformation frequency. But I don’t believe her as the cells are cushioned by water during centrifugation. Can you tell…
Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but my protein is not induced; the Western blot is empty. I sequenced a part of…
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