Quantcast

Cloning & Expression

Benefits of the GUS Gene Reporter System in Plants

Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS,  is short for glucuronidase, an enzyme in the bacterium E. coli. GUS is a good reporter for plants, as it does not occur naturally, and thus, has a low background. With…

Read More

Optimize Bacterial Protein Expression by Considering these 4 Variables

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first.  Now, it’s time to put your expression plasmid into E. coli and get…

Read More

2015 Staff Picks: Cloning and Expression

We’re in the week between the 25th and 1st—the holiday week. If you’re in a college town, traffic is easy and parking restrictions have been lifted. With the staff picks of articles from the Cloning and Expression channel, it really is the most wonderful time of the year. Jen Redig, Managing Editor 8 Basic Points…

Read More