Cloning and Expression
Selection Pressures: Alternatives to Antibiotics in Molecular Cloning
Want to reduce the use of antibiotics in the lab? Start with switching to alternative cloning methods that use alternative selection pressures.
Read More Understanding and Designing Flanking Homology DNA Assembly Experiments
Understanding and Designing Flanking Homology DNA Assembly Experiments Join us in this webinar on demystifying DNA assembly.In this tutorial, you will find: How flanking homology DNA assembly methods work How to use web-based software to design experimental methods for flanking homology DNA assembly methods How synthetic DNA fragments fit in to the DNA assembly process…
Read More Benefits of the GUS Gene Reporter System in Plants
Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS, is short for glucuronidase, an enzyme in the bacterium E. coli. GUS is a good reporter for plants, as it does not occur naturally, and thus, has a low background. With…
Read MoreOptimize Bacterial Protein Expression by Considering these 4 Variables
So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first. Now, it’s time to put your expression plasmid into E. coli and get…
Read More Cut My Gene into Pieces– Introduction to Restriction Enzyme Cloning
At the heart of cloning are restriction enzymes. Restriction enzymes are a common tool in any molecular biology lab. Need to know how large your plasmid is? Cut it with a restriction enzyme. Need to chop your genomic DNA into smaller pieces for a southern hybridization or to prepare a library? Use a restriction enzyme.…
Read More 2015 Staff Picks: Cloning and Expression
We’re in the week between the 25th and 1st—the holiday week. If you’re in a college town, traffic is easy and parking restrictions have been lifted. With the staff picks of articles from the Cloning and Expression channel, it really is the most wonderful time of the year. Jen Redig, Managing Editor 8 Basic Points…
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