Cloning and Expression

Improving the Expression of Insoluble and Inactive Proteins Using Osmotic Shock

Published July 6, 2021

An image of a man punching liquid to depict improving protein expression using osmotic shock

Discover a better way to improve the expression of your insoluble and inactive proteins using osmotic shock.

Unnatural Amino Acids: Essential Tools in Synthetic Biology and Your Research

Published May 25, 2021

Image of chalkboard with the words amino acids surrounded by health foods to juxtapose the topic of unnatural amino acids

Discover what unnatural amino acids are, their applications, and how they can be used in your research in our beginner’s guide.

Selection Pressures: Alternatives to Antibiotics in Molecular Cloning

Published July 14, 2020

A hand selecting a wooden figure with a magent, representing selection in molecular cloning

Want to reduce the use of antibiotics in the lab? Start with switching to alternative cloning methods that use alternative selection pressures.

Optimize Bacterial Protein Expression by Considering these 4 Variables

Published November 30, 2016

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first. Now, it’s time to put your expression plasmid into E. coli and get…