Following closely on the heels of Cristy’s article “How to Clean a Waterbath”, I’d like to take a moment to rant about a few other hated (and carefully avoided) lab tasks. Here are my top ten LEAST favorite things to do in the lab:
Cleaning out the vacuum trap – truly gag-worthy…you never know what your colleagues have been sucking up in there.
Defrosting the -20°C freezer – it’s slow, it’s messy, and you’ll just have to do it all over again in a month or two.
Defrosting the -80°C freezer – even worse, due to the possibility of accidentally freezing body parts to the inside of the freezer, or losing a finger to frostbite.
Filling the liquid nitrogen tank – I always feel like I’m taking my life in my hands when I do this! Something that is so dangerous shouldn’t slosh so much, you know?
Updating the chemical inventory – this would be so much easier if you just updated it every time you ordered a chemical, but when has that ever happened?
Annotating plasmid maps – carefully piecing together the sequence for the clone you just made sometimes seems like it takes even longer than making the clone itself!
Making competent cells – the centrifugations, the aliquoting, stressing out over sterile technique…it doesn’t get any better than this!
Aliquoting – competent cells, antibodies, dNTPs…just about anything, really.
Making up 10M HCl or NaOH stocks for adjusting the pH of solutions – scary scary scary.
Racking pipette tips – have you ever been in a lab cheap enough to buy loose pipette tips that need to be individually inserted into the tip boxes before autoclaving? A sure-fire way to drive your summer student crazy.
A wide variety of enzymes are available for PCR and RT-PCR and the optimal choice depends on a range of factors specific to your experiment. Some of these factors will now be explored to help you to make the most suitable and cost effective choices when ordering. PCR Type and Other Factors to Consider First, […]
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