Top 10 Most Hated Lab Tasks

by on 25th of April, 2012 in Organization & Productivity
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Following closely on the heels of Cristy’s article “How to Clean a Waterbath”, I’d like to take a moment to rant about a few other hated (and carefully avoided) lab tasks.  Here are my top ten LEAST favorite things to do in the lab:

  1. Cleaning out the vacuum trap – truly gag-worthy…you never know what your colleagues have been sucking up in there.
  2. Defrosting the -20°C freezer – it’s slow, it’s messy, and you’ll just have to do it all over again in a month or two.
  3. Defrosting the -80°C freezer – even worse, due to the possibility of accidentally freezing body parts to the inside of the freezer, or losing a finger to frostbite.
  4. Filling the liquid nitrogen tank – I always feel like I’m taking my life in my hands when I do this!  Something that is so dangerous shouldn’t slosh so much, you know?
  5. Updating the chemical inventory – this would be so much easier if you just updated it every time you ordered a chemical, but when has that ever happened?
  6. Annotating plasmid maps – carefully piecing together the sequence for the clone you just made sometimes seems like it takes even longer than making the clone itself!
  7. Making competent cells – the centrifugations, the aliquoting, stressing out over sterile technique…it doesn’t get any better than this!
  8. Aliquoting – competent cells, antibodies, dNTPs…just about anything, really.
  9. Making up 10M HCl or NaOH stocks for adjusting the pH of solutions – scary scary scary.
  10. Racking pipette tips – have you ever been in a lab cheap enough to buy loose pipette tips that need to be individually inserted into the tip boxes before autoclaving?  A sure-fire way to drive your summer student crazy.

What’s YOUR most hated lab task?

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13 thoughts on “Top 10 Most Hated Lab Tasks”

  1. Avatar of micronaut micronaut says:

    11. Calibrating the lab balances.

  2. Avatar of addinsell addinsell says:

    Boxing tips was the bane of my life during a summer studentship a few years ago. Between that and updating a chemical inventory of nearly 1000 compounds I almost lost the will to live. All of the other chores are a joy in comparison.

  3. Avatar of Martin Martin says:

    Top of the gag-worthiness was unblocking the U-bend in the lab sink. I worked in a lab which handled human blood, so there was always a lovely combination of pipette tips along with congealed Virkon/plasma mix. Black pudding anyone ?! Yuck.

  4. Avatar of Kurt Lager Kurt Lager says:

    Buffering phenol
    Picking 5000 colonies

  5. Avatar of coovadia coovadia says:

    12. Calibrating pipettes

  6. 13. Adjusting pH of solutions. One drop too many and you have to start all over again!

  7. Avatar of JJ512 JJ512 says:

    Discarding biohazard waste, YUCK! Always felt like burning my clothes and taking a shower afterward

  8. Avatar of saira saira says:

    I think preparing salt saturated phenol is the most hated work……….

  9. Avatar of Gina Riggio Gina Riggio says:

    Love this article! So funny and exactly what I needed right now. I’d have to say trying to make an RNAse / DNAse free concentrated salt solution for ethanol precipitations. And also making 0.5M EDTA. That shit never dissolves! NEVER!!

    1. Try adding NaOH pellets, while measuring pH. As pH going closer to 8.0, EDTA dissolved. Gently heating while steering helps as well, just don’t leave unattended, it’ll boil.

  10. top ones are boxing tips, adjusting pH and definitely dissolving EDTA.

  11. Avatar of JackBean JackBean says:

    I never realized our lab is cheap, just because we are refilling pipette tips.

    Concerning the chemicals, when I was in Berlin, they had (and still do of course), on-line list of all chemicals, including the private ones, so the list is always up-to-date.

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