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Isoelectric focusing is a way of separating proteins and peptides based on their isoelectric point. Read all about this method and its applications right here.
Pouring and running an agarose gel should be a simple and routine procedure, but there are a surprising number of ways to destroy your agarose gel.
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
It’s not always easy deciding whether to run electrophoresis at a constant voltage, current, or power. Here, we outline the differences to help you make an informed decision.
Enzymes are special among proteins. It is not enough to detect them. You need to know their activity level. If you have devoted a substantial part of your research to studying proteases, like I did, you’ll know how crucial it is to choose an appropriate enzyme assay. There’s a heap of lab techniques out there…
Running a pulsed field gel can be exciting. It isn’t often that you get to visualize such large pieces of DNA. However, it can also be extremely frustrating. It isn’t uncommon to wait 24 hours only to find out that your DNA was degraded before you started the run. Then, you have to start all…
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