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Sigma-Aldrich® Advanced Genomics

CRISPR Screening: from design and discovery to validation

CRISPR Screening: from design and discovery to validation Date: Wednesday 9th December 2020Broadcast 1: 9 am Moscow, 11.30 am Delhi, 2 pm Beijing, 3 pm TokyoBroadcast 2: 4 pm London, 5 pm Berlin, 8 am Los Angeles, 11 am New York Speaker Gillian Browne, PhD Global Head of Commercial Enablement, Genome Engineering & ModulationMilliporeSigma Gillian received…

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Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs

Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs On Demand Speaker Matthew Mackenzie Resource Support ManagerMRC Harwell Matthew Mackenzie earned a BSc in Genetics at the University of Liverpool, where he spent a year in industry with LGC Forensics (now Eurofins) gaining insights into high-throughput sample analysis and automation. After graduating in 2015 he…

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A guide to leveraging single cell CRISPR screens for deeper biological insights

A guide to leveraging single cell CRISPR screens for deeper biological insights On Demand Speakers Andrew Ravanelli, Ph.D. Senior R&D Scientist, Genome and Epigenome Editing, Merck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He…

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Markerless and Scarless Microbial Genome Engineering

Markerless and Scarless Microbial Genome Engineering On Demand Webinar Speaker Erik Eastlund Principal Scientist, Gene Editing and Novel Modalities, Merck KGaA, Darmstadt, Germany Erik is a principal scientist for Gene Editing and Novel Modalities at Merck KGaA, Darmstadt, Germany. His group is focused on developing microbial genome engineering tools while also engaging in a microbiome…

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Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA

Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA On Demand Webinar Dr. Peter Romanienko Managing Director, Genome Editing Shared Resource, Rutgers-Cancer Institute of New Jersey Dr. Peter Romanienko obtained his PhD training from Cornell Graduate School of Medical Sciences/Sloan Kettering Institute working on DNA repair in Dr. Maria Jasin’s laboratory and…

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Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines

Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines On Demand Webinar Dr. Benjamin Borgo Dr. Benjamin Borgo currently leads a team of forward-thinking product and service managers within Merck KGaA’s Genome Engineering and Modulation franchise. His first exposure to CRISPR-based genome editing was as a graduate student where he attempted to…

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CRISPR Nucleases: The Ultimate Guide To Selection

CRISPR nucleases continue to dominate the gene-editing landscape because they are powerful and easy-to-use gene-editing tools. Their rapid discovery and development, however, make choosing the right nuclease for your experiment difficult. This guide highlights the broad range of available CRISPR nucleases and distils the major factors you should consider when choosing one for your next…

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CRISPR Multiplexing Strategies: What’s The Choice?

Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…

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Get Prepped: Nanopore Library Preparation Optimization

Nanopore is a relatively new sequencing platform and researchers are still trying to optimize the protocol for their own specific applications. In our lab, we work primarily with metagenomic samples and use the 1D sequencing kits. Over the past year, we have optimized this technique. To check the quality of the Nanopore library preparation we…

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